Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1541 and SMa1544 are separated by 4 nucleotides SMa1544 and SMa1545 overlap by 1 nucleotides SMa1545 and SMa1547 are separated by 294 nucleotides
SMa1541: SMa1541 - monovalent cation/H+ antiporter subunit D, at 850,844 to 852,307
SMa1541
SMa1544: SMa1544 - NuoK2 NADH:quinone oxidoreductase subunit 11 (chain K), at 852,312 to 852,614
SMa1544
SMa1545: SMa1545 - NuoJ2 NADH:quinone oxidoreductase subunit 6 (chain J), at 852,614 to 853,114
SMa1545
SMa1547: SMa1547 - protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT), at 853,409 to 854,080
SMa1547
Position (kb) 852
853 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 851.327 kb on + strand, within SMa1541 at 851.333 kb on + strand, within SMa1541 at 851.334 kb on - strand, within SMa1541 at 851.334 kb on - strand, within SMa1541 at 851.352 kb on + strand, within SMa1541 at 851.352 kb on + strand, within SMa1541 at 851.353 kb on - strand, within SMa1541 at 851.353 kb on - strand, within SMa1541 at 851.376 kb on + strand, within SMa1541 at 851.376 kb on + strand, within SMa1541 at 851.451 kb on + strand, within SMa1541 at 851.451 kb on + strand, within SMa1541 at 851.451 kb on + strand, within SMa1541 at 851.451 kb on + strand, within SMa1541 at 851.452 kb on - strand, within SMa1541 at 851.746 kb on - strand, within SMa1541 at 851.746 kb on - strand, within SMa1541 at 851.805 kb on + strand, within SMa1541 at 851.806 kb on - strand, within SMa1541 at 851.847 kb on + strand, within SMa1541 at 851.847 kb on + strand, within SMa1541 at 851.848 kb on - strand, within SMa1541 at 851.860 kb on - strand, within SMa1541 at 851.883 kb on + strand, within SMa1541 at 851.883 kb on + strand, within SMa1541 at 851.883 kb on + strand, within SMa1541 at 851.890 kb on + strand, within SMa1541 at 851.891 kb on - strand, within SMa1541 at 851.999 kb on - strand, within SMa1541 at 851.999 kb on - strand, within SMa1541 at 852.000 kb on + strand, within SMa1541 at 852.013 kb on - strand, within SMa1541 at 852.056 kb on + strand, within SMa1541 at 852.056 kb on + strand, within SMa1541 at 852.314 kb on - strand at 852.457 kb on + strand, within SMa1544 at 852.458 kb on - strand, within SMa1544 at 852.460 kb on + strand, within SMa1544 at 852.461 kb on - strand, within SMa1544 at 852.461 kb on - strand, within SMa1544 at 852.936 kb on + strand, within SMa1545 at 852.936 kb on + strand, within SMa1545 at 852.936 kb on + strand, within SMa1545 at 852.937 kb on - strand, within SMa1545 at 852.937 kb on - strand, within SMa1545 at 852.980 kb on - strand, within SMa1545 at 853.168 kb on + strand at 853.194 kb on + strand at 853.194 kb on + strand at 853.194 kb on + strand at 853.217 kb on + strand at 853.217 kb on + strand at 853.277 kb on + strand at 853.278 kb on - strand at 853.278 kb on - strand at 853.337 kb on + strand at 853.338 kb on - strand at 853.338 kb on - strand at 853.359 kb on - strand at 853.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 851,327 + SMa1541 0.33 +2.2 851,333 + SMa1541 0.33 +0.8 851,334 - SMa1541 0.33 +1.5 851,334 - SMa1541 0.33 -0.7 851,352 + SMa1541 0.35 +0.3 851,352 + SMa1541 0.35 +2.2 851,353 - SMa1541 0.35 -0.8 851,353 - SMa1541 0.35 -0.1 851,376 + SMa1541 0.36 -0.4 851,376 + SMa1541 0.36 -0.7 851,451 + SMa1541 0.41 +1.2 851,451 + SMa1541 0.41 +0.8 851,451 + SMa1541 0.41 +0.6 851,451 + SMa1541 0.41 +1.0 851,452 - SMa1541 0.42 +0.9 851,746 - SMa1541 0.62 -0.7 851,746 - SMa1541 0.62 +2.3 851,805 + SMa1541 0.66 +1.3 851,806 - SMa1541 0.66 -0.3 851,847 + SMa1541 0.69 +0.6 851,847 + SMa1541 0.69 -0.2 851,848 - SMa1541 0.69 -1.1 851,860 - SMa1541 0.69 +0.9 851,883 + SMa1541 0.71 -0.0 851,883 + SMa1541 0.71 -1.2 851,883 + SMa1541 0.71 -2.6 851,890 + SMa1541 0.71 -1.4 851,891 - SMa1541 0.72 -2.7 851,999 - SMa1541 0.79 +0.6 851,999 - SMa1541 0.79 -0.4 852,000 + SMa1541 0.79 -0.6 852,013 - SMa1541 0.80 -1.5 852,056 + SMa1541 0.83 +2.9 852,056 + SMa1541 0.83 +0.3 852,314 - +1.1 852,457 + SMa1544 0.48 +1.0 852,458 - SMa1544 0.48 +0.2 852,460 + SMa1544 0.49 -0.3 852,461 - SMa1544 0.49 +0.6 852,461 - SMa1544 0.49 +1.3 852,936 + SMa1545 0.64 +0.7 852,936 + SMa1545 0.64 -0.5 852,936 + SMa1545 0.64 -0.1 852,937 - SMa1545 0.64 -2.1 852,937 - SMa1545 0.64 -0.1 852,980 - SMa1545 0.73 -1.0 853,168 + +0.1 853,194 + -1.3 853,194 + +0.9 853,194 + +0.2 853,217 + +0.8 853,217 + +0.2 853,277 + -1.1 853,278 - +1.0 853,278 - +0.9 853,337 + -4.1 853,338 - +0.2 853,338 - -2.6 853,359 - -0.7 853,476 - +1.5
Or see this region's nucleotide sequence