Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1509 and SMa1513 are separated by 25 nucleotides SMa1513 and SMa1514 overlap by 1 nucleotides SMa1514 and SMa1515 are separated by 45 nucleotides
SMa1509: SMa1509 - ATP transporter ATP-binding protein, at 832,401 to 833,936
SMa1509
SMa1513: SMa1513 - ABC transporter permease, at 833,962 to 834,984
SMa1513
SMa1514: SMa1514 - ABC transporter permease, at 834,984 to 835,904
SMa1514
SMa1515: SMa1515 - hypothetical protein, at 835,950 to 836,141
SMa1515
Position (kb) 833
834
835 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 832.971 kb on + strand, within SMa1509 at 832.972 kb on - strand, within SMa1509 at 832.972 kb on - strand, within SMa1509 at 832.973 kb on + strand, within SMa1509 at 832.973 kb on + strand, within SMa1509 at 833.196 kb on + strand, within SMa1509 at 833.196 kb on + strand, within SMa1509 at 833.197 kb on - strand, within SMa1509 at 833.197 kb on - strand, within SMa1509 at 833.218 kb on + strand, within SMa1509 at 833.218 kb on + strand, within SMa1509 at 833.218 kb on + strand, within SMa1509 at 833.218 kb on + strand, within SMa1509 at 833.218 kb on + strand, within SMa1509 at 833.543 kb on + strand, within SMa1509 at 834.240 kb on - strand, within SMa1513 at 834.383 kb on - strand, within SMa1513 at 834.383 kb on - strand, within SMa1513 at 834.383 kb on - strand, within SMa1513 at 834.448 kb on + strand, within SMa1513 at 834.499 kb on + strand, within SMa1513 at 834.499 kb on + strand, within SMa1513 at 834.586 kb on + strand, within SMa1513 at 834.587 kb on - strand, within SMa1513 at 834.587 kb on - strand, within SMa1513 at 834.628 kb on + strand, within SMa1513 at 834.628 kb on + strand, within SMa1513 at 834.952 kb on + strand at 834.952 kb on + strand at 834.955 kb on + strand at 834.956 kb on - strand at 835.039 kb on + strand at 835.039 kb on + strand at 835.248 kb on + strand, within SMa1514 at 835.249 kb on - strand, within SMa1514 at 835.249 kb on - strand, within SMa1514 at 835.594 kb on - strand, within SMa1514 at 835.594 kb on - strand, within SMa1514 at 835.608 kb on + strand, within SMa1514 at 835.608 kb on + strand, within SMa1514 at 835.608 kb on + strand, within SMa1514 at 835.800 kb on + strand, within SMa1514 at 835.816 kb on + strand at 835.816 kb on + strand at 835.816 kb on + strand at 835.816 kb on + strand at 835.816 kb on + strand at 835.817 kb on - strand at 835.817 kb on - strand at 835.817 kb on - strand at 835.817 kb on - strand at 835.817 kb on - strand at 835.817 kb on - strand at 835.817 kb on - strand at 835.902 kb on + strand at 835.962 kb on + strand at 835.962 kb on + strand at 835.963 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 832,971 + SMa1509 0.37 -2.8 832,972 - SMa1509 0.37 -0.7 832,972 - SMa1509 0.37 +1.0 832,973 + SMa1509 0.37 +0.4 832,973 + SMa1509 0.37 +0.9 833,196 + SMa1509 0.52 -2.4 833,196 + SMa1509 0.52 +0.6 833,197 - SMa1509 0.52 +1.4 833,197 - SMa1509 0.52 -3.4 833,218 + SMa1509 0.53 +0.9 833,218 + SMa1509 0.53 -0.7 833,218 + SMa1509 0.53 +0.9 833,218 + SMa1509 0.53 -0.1 833,218 + SMa1509 0.53 -1.4 833,543 + SMa1509 0.74 +0.0 834,240 - SMa1513 0.27 +0.9 834,383 - SMa1513 0.41 -0.7 834,383 - SMa1513 0.41 +0.5 834,383 - SMa1513 0.41 -3.4 834,448 + SMa1513 0.48 -1.4 834,499 + SMa1513 0.52 -0.7 834,499 + SMa1513 0.52 -1.9 834,586 + SMa1513 0.61 -0.2 834,587 - SMa1513 0.61 -0.9 834,587 - SMa1513 0.61 -1.3 834,628 + SMa1513 0.65 +0.8 834,628 + SMa1513 0.65 -1.1 834,952 + -0.8 834,952 + +2.3 834,955 + +1.1 834,956 - -1.4 835,039 + -3.1 835,039 + +1.2 835,248 + SMa1514 0.29 -1.7 835,249 - SMa1514 0.29 +0.5 835,249 - SMa1514 0.29 -1.2 835,594 - SMa1514 0.66 -3.9 835,594 - SMa1514 0.66 -0.6 835,608 + SMa1514 0.68 +1.2 835,608 + SMa1514 0.68 +1.3 835,608 + SMa1514 0.68 +1.4 835,800 + SMa1514 0.89 -0.7 835,816 + -0.1 835,816 + -2.8 835,816 + +1.7 835,816 + +1.2 835,816 + +0.2 835,817 - -3.4 835,817 - +0.1 835,817 - +1.5 835,817 - -1.7 835,817 - +4.7 835,817 - +1.8 835,817 - -1.4 835,902 + -0.9 835,962 + -0.4 835,962 + +1.2 835,963 - -0.1
Or see this region's nucleotide sequence