Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1400 and SMa1403 are separated by 159 nucleotides SMa1403 and SMa1406 are separated by 114 nucleotides
SMa1400: SMa1400 - acyl-CoA dehydrogenase, at 769,732 to 770,874
SMa1400
SMa1403: SMa1403 - oxidoreductase, at 771,034 to 772,050
SMa1403
SMa1406: SMa1406 - TtuD3 hydroxypyruvate reductase, at 772,165 to 773,424
SMa1406
Position (kb) 771
772
773 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 770.485 kb on + strand, within SMa1400 at 770.520 kb on - strand, within SMa1400 at 770.676 kb on - strand, within SMa1400 at 770.676 kb on - strand, within SMa1400 at 771.208 kb on + strand, within SMa1403 at 771.208 kb on + strand, within SMa1403 at 771.209 kb on - strand, within SMa1403 at 771.271 kb on + strand, within SMa1403 at 771.272 kb on - strand, within SMa1403 at 771.272 kb on - strand, within SMa1403 at 771.376 kb on + strand, within SMa1403 at 771.382 kb on + strand, within SMa1403 at 771.382 kb on + strand, within SMa1403 at 771.382 kb on + strand, within SMa1403 at 771.382 kb on + strand, within SMa1403 at 771.501 kb on - strand, within SMa1403 at 771.656 kb on - strand, within SMa1403 at 771.657 kb on + strand, within SMa1403 at 771.657 kb on + strand, within SMa1403 at 771.658 kb on - strand at 771.658 kb on - strand, within SMa1403 at 771.790 kb on + strand, within SMa1403 at 771.790 kb on + strand, within SMa1403 at 771.791 kb on - strand, within SMa1403 at 771.791 kb on - strand, within SMa1403 at 771.791 kb on - strand, within SMa1403 at 771.813 kb on - strand, within SMa1403 at 771.813 kb on - strand, within SMa1403 at 772.151 kb on - strand at 772.437 kb on + strand, within SMa1406 at 772.674 kb on + strand, within SMa1406
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 770,485 + SMa1400 0.66 -0.9 770,520 - SMa1400 0.69 -1.9 770,676 - SMa1400 0.83 +1.1 770,676 - SMa1400 0.83 -0.9 771,208 + SMa1403 0.17 -0.1 771,208 + SMa1403 0.17 -3.4 771,209 - SMa1403 0.17 -1.7 771,271 + SMa1403 0.23 +2.1 771,272 - SMa1403 0.23 -2.2 771,272 - SMa1403 0.23 -0.4 771,376 + SMa1403 0.34 -0.2 771,382 + SMa1403 0.34 -1.3 771,382 + SMa1403 0.34 -0.6 771,382 + SMa1403 0.34 -3.9 771,382 + SMa1403 0.34 -0.8 771,501 - SMa1403 0.46 -0.9 771,656 - SMa1403 0.61 -0.3 771,657 + SMa1403 0.61 +0.9 771,657 + SMa1403 0.61 +0.9 771,658 - +0.9 771,658 - SMa1403 0.61 -0.1 771,790 + SMa1403 0.74 -1.4 771,790 + SMa1403 0.74 -2.1 771,791 - SMa1403 0.74 +1.9 771,791 - SMa1403 0.74 -2.1 771,791 - SMa1403 0.74 +0.9 771,813 - SMa1403 0.77 -1.1 771,813 - SMa1403 0.77 -0.5 772,151 - +1.5 772,437 + SMa1406 0.22 -1.4 772,674 + SMa1406 0.40 -0.3
Or see this region's nucleotide sequence