Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1362 and SMa1363 overlap by 4 nucleotides SMa1363 and SMa1364 are separated by 63 nucleotides
SMa1362: SMa1362 - inner-membrane permease, at 746,199 to 747,599
SMa1362
SMa1363: SMa1363 - ABC transporter permease, at 747,596 to 748,684
SMa1363
SMa1364: SMa1364 - ABC transporter substrate-binding protein, at 748,748 to 749,968
SMa1364
Position (kb) 747
748
749 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 746.607 kb on + strand, within SMa1362 at 746.607 kb on + strand, within SMa1362 at 746.654 kb on - strand, within SMa1362 at 746.813 kb on + strand, within SMa1362 at 746.814 kb on - strand, within SMa1362 at 746.871 kb on + strand, within SMa1362 at 746.884 kb on + strand, within SMa1362 at 747.024 kb on - strand, within SMa1362 at 747.044 kb on + strand, within SMa1362 at 747.045 kb on - strand, within SMa1362 at 747.045 kb on - strand, within SMa1362 at 747.061 kb on + strand, within SMa1362 at 747.062 kb on - strand, within SMa1362 at 747.182 kb on - strand, within SMa1362 at 747.257 kb on + strand, within SMa1362 at 747.257 kb on + strand, within SMa1362 at 747.258 kb on - strand, within SMa1362 at 747.278 kb on - strand, within SMa1362 at 747.397 kb on + strand, within SMa1362 at 747.442 kb on - strand, within SMa1362 at 747.444 kb on - strand, within SMa1362 at 747.444 kb on - strand, within SMa1362 at 747.499 kb on + strand at 747.499 kb on + strand at 747.500 kb on - strand at 747.501 kb on + strand at 747.652 kb on + strand at 747.653 kb on - strand at 747.653 kb on - strand at 747.730 kb on - strand, within SMa1363 at 747.735 kb on + strand, within SMa1363 at 747.736 kb on - strand, within SMa1363 at 747.738 kb on + strand, within SMa1363 at 747.779 kb on + strand, within SMa1363 at 747.806 kb on + strand, within SMa1363 at 747.807 kb on - strand, within SMa1363 at 747.807 kb on - strand, within SMa1363 at 747.869 kb on + strand, within SMa1363 at 747.869 kb on + strand, within SMa1363 at 747.869 kb on + strand, within SMa1363 at 747.869 kb on + strand, within SMa1363 at 747.869 kb on + strand, within SMa1363 at 747.870 kb on - strand, within SMa1363 at 747.921 kb on + strand, within SMa1363 at 747.922 kb on - strand, within SMa1363 at 747.924 kb on - strand, within SMa1363 at 747.998 kb on + strand, within SMa1363 at 748.052 kb on + strand, within SMa1363 at 748.072 kb on + strand, within SMa1363 at 748.072 kb on + strand, within SMa1363 at 748.072 kb on + strand, within SMa1363 at 748.072 kb on + strand, within SMa1363 at 748.072 kb on + strand, within SMa1363 at 748.183 kb on - strand, within SMa1363 at 748.183 kb on - strand, within SMa1363 at 748.522 kb on - strand, within SMa1363 at 748.733 kb on + strand at 748.891 kb on + strand, within SMa1364 at 748.891 kb on + strand, within SMa1364 at 748.892 kb on - strand, within SMa1364 at 748.981 kb on + strand, within SMa1364 at 748.982 kb on - strand, within SMa1364 at 749.038 kb on + strand, within SMa1364 at 749.038 kb on + strand, within SMa1364 at 749.039 kb on - strand, within SMa1364 at 749.039 kb on - strand, within SMa1364 at 749.042 kb on + strand, within SMa1364 at 749.043 kb on - strand, within SMa1364 at 749.072 kb on - strand, within SMa1364 at 749.228 kb on + strand, within SMa1364 at 749.322 kb on + strand, within SMa1364 at 749.322 kb on + strand, within SMa1364 at 749.350 kb on + strand, within SMa1364 at 749.357 kb on + strand, within SMa1364 at 749.424 kb on + strand, within SMa1364 at 749.424 kb on + strand, within SMa1364 at 749.575 kb on + strand, within SMa1364 at 749.575 kb on + strand, within SMa1364 at 749.598 kb on + strand, within SMa1364
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 746,607 + SMa1362 0.29 +2.9 746,607 + SMa1362 0.29 +1.9 746,654 - SMa1362 0.32 -0.4 746,813 + SMa1362 0.44 -3.1 746,814 - SMa1362 0.44 +0.6 746,871 + SMa1362 0.48 -1.7 746,884 + SMa1362 0.49 -1.7 747,024 - SMa1362 0.59 +1.4 747,044 + SMa1362 0.60 -0.1 747,045 - SMa1362 0.60 +0.8 747,045 - SMa1362 0.60 -0.2 747,061 + SMa1362 0.62 +0.4 747,062 - SMa1362 0.62 +0.5 747,182 - SMa1362 0.70 +0.3 747,257 + SMa1362 0.76 -1.0 747,257 + SMa1362 0.76 +0.2 747,258 - SMa1362 0.76 -0.6 747,278 - SMa1362 0.77 -0.1 747,397 + SMa1362 0.86 -1.9 747,442 - SMa1362 0.89 -0.7 747,444 - SMa1362 0.89 -1.7 747,444 - SMa1362 0.89 -0.4 747,499 + -2.7 747,499 + +0.9 747,500 - -1.8 747,501 + +4.7 747,652 + -0.6 747,653 - +2.1 747,653 - -2.1 747,730 - SMa1363 0.12 +0.5 747,735 + SMa1363 0.13 -0.7 747,736 - SMa1363 0.13 -0.1 747,738 + SMa1363 0.13 -0.7 747,779 + SMa1363 0.17 -1.3 747,806 + SMa1363 0.19 -1.4 747,807 - SMa1363 0.19 +0.2 747,807 - SMa1363 0.19 +0.8 747,869 + SMa1363 0.25 -2.4 747,869 + SMa1363 0.25 -0.9 747,869 + SMa1363 0.25 +2.7 747,869 + SMa1363 0.25 -0.7 747,869 + SMa1363 0.25 +0.2 747,870 - SMa1363 0.25 +0.3 747,921 + SMa1363 0.30 -0.1 747,922 - SMa1363 0.30 -1.7 747,924 - SMa1363 0.30 -2.6 747,998 + SMa1363 0.37 -3.3 748,052 + SMa1363 0.42 -1.4 748,072 + SMa1363 0.44 -2.1 748,072 + SMa1363 0.44 -0.8 748,072 + SMa1363 0.44 -0.6 748,072 + SMa1363 0.44 -0.9 748,072 + SMa1363 0.44 +0.8 748,183 - SMa1363 0.54 +0.1 748,183 - SMa1363 0.54 +1.2 748,522 - SMa1363 0.85 -1.2 748,733 + +2.0 748,891 + SMa1364 0.12 +1.4 748,891 + SMa1364 0.12 -2.3 748,892 - SMa1364 0.12 -1.4 748,981 + SMa1364 0.19 +1.9 748,982 - SMa1364 0.19 -2.7 749,038 + SMa1364 0.24 +0.9 749,038 + SMa1364 0.24 +0.3 749,039 - SMa1364 0.24 +0.1 749,039 - SMa1364 0.24 +0.6 749,042 + SMa1364 0.24 +0.4 749,043 - SMa1364 0.24 +0.9 749,072 - SMa1364 0.27 -1.1 749,228 + SMa1364 0.39 -3.3 749,322 + SMa1364 0.47 +0.6 749,322 + SMa1364 0.47 +0.2 749,350 + SMa1364 0.49 +3.5 749,357 + SMa1364 0.50 -0.1 749,424 + SMa1364 0.55 +1.6 749,424 + SMa1364 0.55 -0.1 749,575 + SMa1364 0.68 -0.4 749,575 + SMa1364 0.68 +0.1 749,598 + SMa1364 0.70 -0.0
Or see this region's nucleotide sequence