Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1318 and SMa1319 are separated by 4 nucleotides SMa1319 and SMa1321 overlap by 4 nucleotides SMa1321 and SMa1322 are separated by 3 nucleotides SMa1322 and SMa1323 are separated by 96 nucleotides SMa1323 and SMa1325 are separated by 260 nucleotides
SMa1318: SMa1318 - VirB3 type IV secretion protein, at 719,504 to 719,845
SMa1318
SMa1319: SMa1319 - VirB2 type IV secretion protein, at 719,850 to 720,149
SMa1319
SMa1321: SMa1321 - VirB1 type IV secretion protein, at 720,146 to 720,817
SMa1321
SMa1322: SMa1322 - hypothetical protein, at 720,821 to 721,339
SMa1322
SMa1323: SMa1323 - Transcription regulator, at 721,436 to 721,804
SMa1323
SMa1325: SMa1325 - hypothetical protein, at 722,065 to 722,316
SMa1325
Position (kb)
720
721
722 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 719.941 kb on + strand, within SMa1319 at 720.005 kb on + strand, within SMa1319 at 720.429 kb on + strand, within SMa1321 at 720.429 kb on + strand, within SMa1321 at 720.430 kb on - strand, within SMa1321 at 720.432 kb on + strand, within SMa1321 at 720.432 kb on + strand, within SMa1321 at 720.499 kb on - strand, within SMa1321 at 720.499 kb on - strand, within SMa1321 at 720.773 kb on + strand at 720.773 kb on + strand at 720.774 kb on - strand at 720.774 kb on - strand at 720.775 kb on + strand at 720.775 kb on + strand at 720.870 kb on + strand at 720.871 kb on - strand at 720.910 kb on - strand, within SMa1322 at 720.910 kb on - strand, within SMa1322 at 721.024 kb on + strand, within SMa1322 at 721.024 kb on + strand, within SMa1322 at 721.080 kb on + strand, within SMa1322 at 721.081 kb on - strand, within SMa1322 at 721.081 kb on - strand, within SMa1322 at 721.083 kb on + strand, within SMa1322 at 721.093 kb on - strand, within SMa1322 at 721.234 kb on + strand, within SMa1322 at 721.268 kb on - strand, within SMa1322 at 721.284 kb on + strand, within SMa1322 at 721.285 kb on - strand, within SMa1322 at 721.295 kb on + strand at 721.296 kb on - strand at 721.474 kb on - strand, within SMa1323 at 721.572 kb on - strand, within SMa1323 at 721.687 kb on + strand, within SMa1323 at 721.687 kb on + strand, within SMa1323 at 721.768 kb on - strand at 721.823 kb on - strand at 721.918 kb on + strand at 721.918 kb on + strand at 721.918 kb on + strand at 721.919 kb on - strand at 721.922 kb on - strand at 722.206 kb on + strand, within SMa1325 at 722.253 kb on - strand, within SMa1325 at 722.299 kb on + strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.300 kb on - strand at 722.324 kb on + strand at 722.325 kb on - strand at 722.335 kb on + strand at 722.336 kb on - strand at 722.336 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 719,941 + SMa1319 0.30 +0.0 720,005 + SMa1319 0.52 -0.8 720,429 + SMa1321 0.42 -2.3 720,429 + SMa1321 0.42 -2.5 720,430 - SMa1321 0.42 -2.9 720,432 + SMa1321 0.43 +0.3 720,432 + SMa1321 0.43 +0.5 720,499 - SMa1321 0.53 +0.6 720,499 - SMa1321 0.53 -1.1 720,773 + -0.7 720,773 + -1.5 720,774 - +1.8 720,774 - +1.0 720,775 + +1.1 720,775 + +0.8 720,870 + -4.3 720,871 - -1.7 720,910 - SMa1322 0.17 -2.1 720,910 - SMa1322 0.17 -2.0 721,024 + SMa1322 0.39 +0.3 721,024 + SMa1322 0.39 -1.7 721,080 + SMa1322 0.50 +1.0 721,081 - SMa1322 0.50 -2.2 721,081 - SMa1322 0.50 -2.6 721,083 + SMa1322 0.50 -3.2 721,093 - SMa1322 0.52 +1.1 721,234 + SMa1322 0.80 +0.5 721,268 - SMa1322 0.86 -0.4 721,284 + SMa1322 0.89 -0.7 721,285 - SMa1322 0.89 -0.0 721,295 + +0.5 721,296 - -0.3 721,474 - SMa1323 0.10 -2.8 721,572 - SMa1323 0.37 +0.6 721,687 + SMa1323 0.68 -2.1 721,687 + SMa1323 0.68 -0.1 721,768 - -0.3 721,823 - +0.0 721,918 + +0.3 721,918 + +0.2 721,918 + +1.2 721,919 - +0.7 721,922 - -0.4 722,206 + SMa1325 0.56 +0.2 722,253 - SMa1325 0.75 +0.4 722,299 + -1.6 722,300 - -0.1 722,300 - -1.7 722,300 - -0.5 722,300 - -0.3 722,300 - -0.9 722,324 + -0.3 722,325 - +2.5 722,335 + +0.5 722,336 - -0.2 722,336 - +0.9
Or see this region's nucleotide sequence