Strain Fitness in Sinorhizobium meliloti 1021 around SMa1322

Experiment: Polymyxin B sulfate 0.00005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSMa1318 and SMa1319 are separated by 4 nucleotidesSMa1319 and SMa1321 overlap by 4 nucleotidesSMa1321 and SMa1322 are separated by 3 nucleotidesSMa1322 and SMa1323 are separated by 96 nucleotidesSMa1323 and SMa1325 are separated by 260 nucleotides SMa1318: SMa1318 - VirB3 type IV secretion protein, at 719,504 to 719,845 SMa1318 SMa1319: SMa1319 - VirB2 type IV secretion protein, at 719,850 to 720,149 SMa1319 SMa1321: SMa1321 - VirB1 type IV secretion protein, at 720,146 to 720,817 SMa1321 SMa1322: SMa1322 - hypothetical protein, at 720,821 to 721,339 SMa1322 SMa1323: SMa1323 - Transcription regulator, at 721,436 to 721,804 SMa1323 SMa1325: SMa1325 - hypothetical protein, at 722,065 to 722,316 SMa1325 Position (kb) 720 721 722Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 719.941 kb on + strand, within SMa1319at 720.005 kb on + strand, within SMa1319at 720.429 kb on + strand, within SMa1321at 720.429 kb on + strand, within SMa1321at 720.430 kb on - strand, within SMa1321at 720.432 kb on + strand, within SMa1321at 720.432 kb on + strand, within SMa1321at 720.499 kb on - strand, within SMa1321at 720.499 kb on - strand, within SMa1321at 720.773 kb on + strandat 720.773 kb on + strandat 720.774 kb on - strandat 720.774 kb on - strandat 720.775 kb on + strandat 720.775 kb on + strandat 720.870 kb on + strandat 720.871 kb on - strandat 720.910 kb on - strand, within SMa1322at 720.910 kb on - strand, within SMa1322at 721.024 kb on + strand, within SMa1322at 721.024 kb on + strand, within SMa1322at 721.080 kb on + strand, within SMa1322at 721.081 kb on - strand, within SMa1322at 721.081 kb on - strand, within SMa1322at 721.083 kb on + strand, within SMa1322at 721.093 kb on - strand, within SMa1322at 721.234 kb on + strand, within SMa1322at 721.268 kb on - strand, within SMa1322at 721.284 kb on + strand, within SMa1322at 721.285 kb on - strand, within SMa1322at 721.295 kb on + strandat 721.296 kb on - strandat 721.474 kb on - strand, within SMa1323at 721.572 kb on - strand, within SMa1323at 721.687 kb on + strand, within SMa1323at 721.687 kb on + strand, within SMa1323at 721.768 kb on - strandat 721.823 kb on - strandat 721.918 kb on + strandat 721.918 kb on + strandat 721.918 kb on + strandat 721.919 kb on - strandat 721.922 kb on - strandat 722.206 kb on + strand, within SMa1325at 722.253 kb on - strand, within SMa1325at 722.299 kb on + strandat 722.300 kb on - strandat 722.300 kb on - strandat 722.300 kb on - strandat 722.300 kb on - strandat 722.300 kb on - strandat 722.324 kb on + strandat 722.325 kb on - strandat 722.335 kb on + strandat 722.336 kb on - strandat 722.336 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml
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719,941 + SMa1319 0.30 +0.0
720,005 + SMa1319 0.52 -0.8
720,429 + SMa1321 0.42 -2.3
720,429 + SMa1321 0.42 -2.5
720,430 - SMa1321 0.42 -2.9
720,432 + SMa1321 0.43 +0.3
720,432 + SMa1321 0.43 +0.5
720,499 - SMa1321 0.53 +0.6
720,499 - SMa1321 0.53 -1.1
720,773 + -0.7
720,773 + -1.5
720,774 - +1.8
720,774 - +1.0
720,775 + +1.1
720,775 + +0.8
720,870 + -4.3
720,871 - -1.7
720,910 - SMa1322 0.17 -2.1
720,910 - SMa1322 0.17 -2.0
721,024 + SMa1322 0.39 +0.3
721,024 + SMa1322 0.39 -1.7
721,080 + SMa1322 0.50 +1.0
721,081 - SMa1322 0.50 -2.2
721,081 - SMa1322 0.50 -2.6
721,083 + SMa1322 0.50 -3.2
721,093 - SMa1322 0.52 +1.1
721,234 + SMa1322 0.80 +0.5
721,268 - SMa1322 0.86 -0.4
721,284 + SMa1322 0.89 -0.7
721,285 - SMa1322 0.89 -0.0
721,295 + +0.5
721,296 - -0.3
721,474 - SMa1323 0.10 -2.8
721,572 - SMa1323 0.37 +0.6
721,687 + SMa1323 0.68 -2.1
721,687 + SMa1323 0.68 -0.1
721,768 - -0.3
721,823 - +0.0
721,918 + +0.3
721,918 + +0.2
721,918 + +1.2
721,919 - +0.7
721,922 - -0.4
722,206 + SMa1325 0.56 +0.2
722,253 - SMa1325 0.75 +0.4
722,299 + -1.6
722,300 - -0.1
722,300 - -1.7
722,300 - -0.5
722,300 - -0.3
722,300 - -0.9
722,324 + -0.3
722,325 - +2.5
722,335 + +0.5
722,336 - -0.2
722,336 - +0.9

Or see this region's nucleotide sequence