Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1315 and SMa1318 overlap by 8 nucleotides SMa1318 and SMa1319 are separated by 4 nucleotides SMa1319 and SMa1321 overlap by 4 nucleotides SMa1321 and SMa1322 are separated by 3 nucleotides
SMa1315: SMa1315 - VirB4 type IV secretion protein, at 717,133 to 719,511
SMa1315
SMa1318: SMa1318 - VirB3 type IV secretion protein, at 719,504 to 719,845
SMa1318
SMa1319: SMa1319 - VirB2 type IV secretion protein, at 719,850 to 720,149
SMa1319
SMa1321: SMa1321 - VirB1 type IV secretion protein, at 720,146 to 720,817
SMa1321
SMa1322: SMa1322 - hypothetical protein, at 720,821 to 721,339
SMa1322
Position (kb) 719
720
721 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 718.856 kb on + strand, within SMa1315 at 718.857 kb on - strand, within SMa1315 at 719.153 kb on + strand, within SMa1315 at 719.153 kb on + strand, within SMa1315 at 719.154 kb on - strand, within SMa1315 at 719.199 kb on - strand, within SMa1315 at 719.202 kb on + strand, within SMa1315 at 719.202 kb on + strand, within SMa1315 at 719.202 kb on + strand, within SMa1315 at 719.202 kb on + strand, within SMa1315 at 719.202 kb on + strand, within SMa1315 at 719.202 kb on + strand, within SMa1315 at 719.203 kb on - strand, within SMa1315 at 719.203 kb on - strand, within SMa1315 at 719.359 kb on - strand at 719.454 kb on - strand at 719.506 kb on - strand at 719.506 kb on - strand at 719.532 kb on + strand at 719.558 kb on - strand, within SMa1318 at 719.721 kb on + strand, within SMa1318 at 719.721 kb on + strand, within SMa1318 at 719.722 kb on - strand, within SMa1318 at 719.769 kb on - strand, within SMa1318 at 719.941 kb on + strand, within SMa1319 at 720.005 kb on + strand, within SMa1319 at 720.429 kb on + strand, within SMa1321 at 720.429 kb on + strand, within SMa1321 at 720.430 kb on - strand, within SMa1321 at 720.432 kb on + strand, within SMa1321 at 720.432 kb on + strand, within SMa1321 at 720.499 kb on - strand, within SMa1321 at 720.499 kb on - strand, within SMa1321 at 720.773 kb on + strand at 720.773 kb on + strand at 720.774 kb on - strand at 720.774 kb on - strand at 720.775 kb on + strand at 720.775 kb on + strand at 720.870 kb on + strand at 720.871 kb on - strand at 720.910 kb on - strand, within SMa1322 at 720.910 kb on - strand, within SMa1322 at 721.024 kb on + strand, within SMa1322 at 721.024 kb on + strand, within SMa1322 at 721.080 kb on + strand, within SMa1322 at 721.081 kb on - strand, within SMa1322 at 721.081 kb on - strand, within SMa1322 at 721.083 kb on + strand, within SMa1322 at 721.093 kb on - strand, within SMa1322
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 718,856 + SMa1315 0.72 +1.8 718,857 - SMa1315 0.72 -0.2 719,153 + SMa1315 0.85 -0.3 719,153 + SMa1315 0.85 +2.0 719,154 - SMa1315 0.85 +0.4 719,199 - SMa1315 0.87 -0.3 719,202 + SMa1315 0.87 -0.5 719,202 + SMa1315 0.87 -0.3 719,202 + SMa1315 0.87 +0.9 719,202 + SMa1315 0.87 +2.0 719,202 + SMa1315 0.87 -0.7 719,202 + SMa1315 0.87 +1.2 719,203 - SMa1315 0.87 +1.4 719,203 - SMa1315 0.87 +1.3 719,359 - -3.6 719,454 - +1.0 719,506 - -0.7 719,506 - +0.3 719,532 + -1.5 719,558 - SMa1318 0.16 -0.2 719,721 + SMa1318 0.63 +2.1 719,721 + SMa1318 0.63 +1.1 719,722 - SMa1318 0.64 -1.2 719,769 - SMa1318 0.77 -2.4 719,941 + SMa1319 0.30 +0.0 720,005 + SMa1319 0.52 -0.8 720,429 + SMa1321 0.42 -2.3 720,429 + SMa1321 0.42 -2.5 720,430 - SMa1321 0.42 -2.9 720,432 + SMa1321 0.43 +0.3 720,432 + SMa1321 0.43 +0.5 720,499 - SMa1321 0.53 +0.6 720,499 - SMa1321 0.53 -1.1 720,773 + -0.7 720,773 + -1.5 720,774 - +1.8 720,774 - +1.0 720,775 + +1.1 720,775 + +0.8 720,870 + -4.3 720,871 - -1.7 720,910 - SMa1322 0.17 -2.1 720,910 - SMa1322 0.17 -2.0 721,024 + SMa1322 0.39 +0.3 721,024 + SMa1322 0.39 -1.7 721,080 + SMa1322 0.50 +1.0 721,081 - SMa1322 0.50 -2.2 721,081 - SMa1322 0.50 -2.6 721,083 + SMa1322 0.50 -3.2 721,093 - SMa1322 0.52 +1.1
Or see this region's nucleotide sequence