Strain Fitness in Sinorhizobium meliloti 1021 around SMa1315

Experiment: Polymyxin B sulfate 0.00005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSMa1311 and SMa1313 are separated by 229 nucleotidesSMa1313 and SMa1315 overlap by 1 nucleotidesSMa1315 and SMa1318 overlap by 8 nucleotidesSMa1318 and SMa1319 are separated by 4 nucleotidesSMa1319 and SMa1321 overlap by 4 nucleotides SMa1311: SMa1311 - VirB6 type IV secretion protein, at 715,261 to 716,202 SMa1311 SMa1313: SMa1313 - VirB5 type IV secretion protein, at 716,432 to 717,133 SMa1313 SMa1315: SMa1315 - VirB4 type IV secretion protein, at 717,133 to 719,511 SMa1315 SMa1318: SMa1318 - VirB3 type IV secretion protein, at 719,504 to 719,845 SMa1318 SMa1319: SMa1319 - VirB2 type IV secretion protein, at 719,850 to 720,149 SMa1319 SMa1321: SMa1321 - VirB1 type IV secretion protein, at 720,146 to 720,817 SMa1321 Position (kb) 717 718 719 720Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 716.192 kb on + strandat 716.192 kb on + strandat 716.192 kb on + strandat 716.192 kb on + strandat 716.193 kb on - strandat 716.193 kb on - strandat 716.193 kb on - strandat 716.365 kb on + strandat 716.366 kb on - strandat 716.366 kb on - strandat 716.402 kb on + strandat 716.402 kb on + strandat 716.547 kb on - strand, within SMa1313at 716.679 kb on + strand, within SMa1313at 716.745 kb on + strand, within SMa1313at 716.745 kb on + strand, within SMa1313at 716.773 kb on - strand, within SMa1313at 716.779 kb on + strand, within SMa1313at 716.779 kb on + strand, within SMa1313at 716.780 kb on - strand, within SMa1313at 717.116 kb on - strandat 717.116 kb on - strandat 717.298 kb on - strandat 717.392 kb on + strand, within SMa1315at 717.653 kb on + strand, within SMa1315at 717.653 kb on + strand, within SMa1315at 717.653 kb on + strand, within SMa1315at 717.653 kb on + strand, within SMa1315at 717.654 kb on - strand, within SMa1315at 717.708 kb on - strand, within SMa1315at 718.007 kb on - strand, within SMa1315at 718.064 kb on + strand, within SMa1315at 718.064 kb on + strand, within SMa1315at 718.065 kb on - strand, within SMa1315at 718.065 kb on - strand, within SMa1315at 718.065 kb on - strand, within SMa1315at 718.088 kb on + strand, within SMa1315at 718.111 kb on - strand, within SMa1315at 718.144 kb on + strand, within SMa1315at 718.241 kb on + strand, within SMa1315at 718.241 kb on + strand, within SMa1315at 718.381 kb on - strand, within SMa1315at 718.399 kb on + strand, within SMa1315at 718.793 kb on + strand, within SMa1315at 718.794 kb on - strand, within SMa1315at 718.856 kb on + strand, within SMa1315at 718.857 kb on - strand, within SMa1315at 719.153 kb on + strand, within SMa1315at 719.153 kb on + strand, within SMa1315at 719.154 kb on - strand, within SMa1315at 719.199 kb on - strand, within SMa1315at 719.202 kb on + strand, within SMa1315at 719.202 kb on + strand, within SMa1315at 719.202 kb on + strand, within SMa1315at 719.202 kb on + strand, within SMa1315at 719.202 kb on + strand, within SMa1315at 719.202 kb on + strand, within SMa1315at 719.203 kb on - strand, within SMa1315at 719.203 kb on - strand, within SMa1315at 719.359 kb on - strandat 719.454 kb on - strandat 719.506 kb on - strandat 719.506 kb on - strandat 719.532 kb on + strandat 719.558 kb on - strand, within SMa1318at 719.721 kb on + strand, within SMa1318at 719.721 kb on + strand, within SMa1318at 719.722 kb on - strand, within SMa1318at 719.769 kb on - strand, within SMa1318at 719.941 kb on + strand, within SMa1319at 720.005 kb on + strand, within SMa1319at 720.429 kb on + strand, within SMa1321at 720.429 kb on + strand, within SMa1321at 720.430 kb on - strand, within SMa1321at 720.432 kb on + strand, within SMa1321at 720.432 kb on + strand, within SMa1321at 720.499 kb on - strand, within SMa1321at 720.499 kb on - strand, within SMa1321

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml
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716,192 + +0.4
716,192 + +2.0
716,192 + +0.7
716,192 + +0.1
716,193 - +5.3
716,193 - -1.5
716,193 - +1.3
716,365 + -1.0
716,366 - +2.6
716,366 - +0.4
716,402 + -1.6
716,402 + +3.2
716,547 - SMa1313 0.16 -1.7
716,679 + SMa1313 0.35 -2.4
716,745 + SMa1313 0.45 +0.9
716,745 + SMa1313 0.45 -2.2
716,773 - SMa1313 0.49 -2.3
716,779 + SMa1313 0.49 +1.0
716,779 + SMa1313 0.49 +0.4
716,780 - SMa1313 0.50 -2.7
717,116 - -1.5
717,116 - -1.6
717,298 - -0.1
717,392 + SMa1315 0.11 -1.0
717,653 + SMa1315 0.22 +2.4
717,653 + SMa1315 0.22 +2.0
717,653 + SMa1315 0.22 -0.4
717,653 + SMa1315 0.22 -0.1
717,654 - SMa1315 0.22 +1.1
717,708 - SMa1315 0.24 -1.1
718,007 - SMa1315 0.37 +1.8
718,064 + SMa1315 0.39 -1.5
718,064 + SMa1315 0.39 +0.9
718,065 - SMa1315 0.39 -1.6
718,065 - SMa1315 0.39 +0.3
718,065 - SMa1315 0.39 +3.4
718,088 + SMa1315 0.40 +0.7
718,111 - SMa1315 0.41 +0.1
718,144 + SMa1315 0.42 -1.1
718,241 + SMa1315 0.47 -1.5
718,241 + SMa1315 0.47 -1.6
718,381 - SMa1315 0.52 +0.0
718,399 + SMa1315 0.53 -0.0
718,793 + SMa1315 0.70 +0.9
718,794 - SMa1315 0.70 -1.6
718,856 + SMa1315 0.72 +1.8
718,857 - SMa1315 0.72 -0.2
719,153 + SMa1315 0.85 -0.3
719,153 + SMa1315 0.85 +2.0
719,154 - SMa1315 0.85 +0.4
719,199 - SMa1315 0.87 -0.3
719,202 + SMa1315 0.87 -0.5
719,202 + SMa1315 0.87 -0.3
719,202 + SMa1315 0.87 +0.9
719,202 + SMa1315 0.87 +2.0
719,202 + SMa1315 0.87 -0.7
719,202 + SMa1315 0.87 +1.2
719,203 - SMa1315 0.87 +1.4
719,203 - SMa1315 0.87 +1.3
719,359 - -3.6
719,454 - +1.0
719,506 - -0.7
719,506 - +0.3
719,532 + -1.5
719,558 - SMa1318 0.16 -0.2
719,721 + SMa1318 0.63 +2.1
719,721 + SMa1318 0.63 +1.1
719,722 - SMa1318 0.64 -1.2
719,769 - SMa1318 0.77 -2.4
719,941 + SMa1319 0.30 +0.0
720,005 + SMa1319 0.52 -0.8
720,429 + SMa1321 0.42 -2.3
720,429 + SMa1321 0.42 -2.5
720,430 - SMa1321 0.42 -2.9
720,432 + SMa1321 0.43 +0.3
720,432 + SMa1321 0.43 +0.5
720,499 - SMa1321 0.53 +0.6
720,499 - SMa1321 0.53 -1.1

Or see this region's nucleotide sequence