Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1269 and SMa1272 are separated by 4 nucleotides SMa1272 and SMa1273 are separated by 59 nucleotides SMa1273 and SMa1276 are separated by 24 nucleotides SMa1276 and SMa1279 are separated by 458 nucleotides
SMa1269: SMa1269 - NorD accessory protein for nitric oxide reductase, at 693,908 to 695,803
SMa1269
SMa1272: SMa1272 - NorQ accessory protein for nitric oxide reductase, at 695,808 to 696,626
SMa1272
SMa1273: SMa1273 - NorB nitric oxide reductase, large subunit, at 696,686 to 698,032
SMa1273
SMa1276: SMa1276 - NorC nitric oxide reductase, small subunit, at 698,057 to 698,509
SMa1276
SMa1279: SMa1279 - NorE accessory protein for nitric oxide reductase, at 698,968 to 699,534
SMa1279
Position (kb) 696
697
698
699 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 695.741 kb on - strand at 695.805 kb on + strand at 695.806 kb on - strand at 695.810 kb on - strand at 695.945 kb on - strand, within SMa1272 at 695.945 kb on - strand, within SMa1272 at 696.000 kb on + strand, within SMa1272 at 696.310 kb on + strand, within SMa1272 at 696.464 kb on + strand, within SMa1272 at 696.574 kb on + strand at 696.626 kb on + strand at 696.632 kb on - strand at 696.725 kb on - strand at 696.779 kb on + strand at 696.779 kb on + strand at 696.780 kb on - strand at 696.967 kb on - strand, within SMa1273 at 696.967 kb on - strand, within SMa1273 at 696.967 kb on - strand, within SMa1273 at 697.000 kb on - strand, within SMa1273 at 697.029 kb on + strand, within SMa1273 at 697.030 kb on - strand, within SMa1273 at 697.030 kb on - strand, within SMa1273 at 697.030 kb on - strand, within SMa1273 at 697.086 kb on + strand, within SMa1273 at 697.086 kb on + strand, within SMa1273 at 697.089 kb on + strand, within SMa1273 at 697.089 kb on + strand, within SMa1273 at 697.090 kb on - strand, within SMa1273 at 697.090 kb on - strand, within SMa1273 at 697.090 kb on - strand, within SMa1273 at 697.090 kb on - strand, within SMa1273 at 697.090 kb on - strand, within SMa1273 at 697.108 kb on + strand, within SMa1273 at 697.315 kb on - strand, within SMa1273 at 697.315 kb on - strand, within SMa1273 at 697.317 kb on + strand, within SMa1273 at 697.371 kb on + strand, within SMa1273 at 697.371 kb on + strand, within SMa1273 at 697.371 kb on + strand, within SMa1273 at 697.371 kb on + strand, within SMa1273 at 697.372 kb on - strand, within SMa1273 at 697.372 kb on - strand, within SMa1273 at 697.372 kb on - strand, within SMa1273 at 697.372 kb on - strand, within SMa1273 at 697.485 kb on + strand, within SMa1273 at 697.486 kb on - strand, within SMa1273 at 697.486 kb on - strand, within SMa1273 at 697.494 kb on + strand, within SMa1273 at 697.494 kb on + strand, within SMa1273 at 697.495 kb on - strand, within SMa1273 at 697.527 kb on + strand, within SMa1273 at 697.527 kb on + strand, within SMa1273 at 697.611 kb on - strand, within SMa1273 at 697.789 kb on - strand, within SMa1273 at 697.789 kb on - strand, within SMa1273 at 697.821 kb on + strand, within SMa1273 at 697.821 kb on + strand, within SMa1273 at 697.824 kb on + strand, within SMa1273 at 697.824 kb on + strand, within SMa1273 at 697.824 kb on + strand, within SMa1273 at 697.824 kb on + strand, within SMa1273 at 697.825 kb on - strand, within SMa1273 at 697.825 kb on - strand, within SMa1273 at 697.932 kb on + strand at 697.932 kb on + strand at 697.933 kb on - strand at 697.998 kb on + strand at 698.290 kb on - strand, within SMa1276 at 698.311 kb on + strand, within SMa1276 at 698.406 kb on + strand, within SMa1276 at 698.407 kb on - strand, within SMa1276 at 698.407 kb on - strand, within SMa1276 at 698.409 kb on + strand, within SMa1276 at 698.409 kb on + strand, within SMa1276 at 698.616 kb on + strand at 698.617 kb on - strand at 698.617 kb on - strand at 698.921 kb on + strand at 698.978 kb on + strand at 698.979 kb on - strand at 698.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 695,741 - +0.2 695,805 + +0.9 695,806 - -3.6 695,810 - -0.6 695,945 - SMa1272 0.17 -0.6 695,945 - SMa1272 0.17 -0.3 696,000 + SMa1272 0.23 +0.6 696,310 + SMa1272 0.61 -1.0 696,464 + SMa1272 0.80 +0.1 696,574 + -2.0 696,626 + +0.6 696,632 - -0.2 696,725 - +0.6 696,779 + +2.4 696,779 + -0.5 696,780 - -0.4 696,967 - SMa1273 0.21 +0.1 696,967 - SMa1273 0.21 -0.4 696,967 - SMa1273 0.21 -1.5 697,000 - SMa1273 0.23 +0.3 697,029 + SMa1273 0.25 -1.0 697,030 - SMa1273 0.26 -3.2 697,030 - SMa1273 0.26 -1.6 697,030 - SMa1273 0.26 -3.2 697,086 + SMa1273 0.30 -1.3 697,086 + SMa1273 0.30 -0.0 697,089 + SMa1273 0.30 -1.0 697,089 + SMa1273 0.30 +0.5 697,090 - SMa1273 0.30 +1.2 697,090 - SMa1273 0.30 +0.7 697,090 - SMa1273 0.30 -0.6 697,090 - SMa1273 0.30 -0.9 697,090 - SMa1273 0.30 -0.1 697,108 + SMa1273 0.31 -1.4 697,315 - SMa1273 0.47 -1.2 697,315 - SMa1273 0.47 -2.3 697,317 + SMa1273 0.47 +1.5 697,371 + SMa1273 0.51 +0.2 697,371 + SMa1273 0.51 +0.4 697,371 + SMa1273 0.51 -0.3 697,371 + SMa1273 0.51 -0.1 697,372 - SMa1273 0.51 +1.2 697,372 - SMa1273 0.51 +3.4 697,372 - SMa1273 0.51 -2.0 697,372 - SMa1273 0.51 -1.1 697,485 + SMa1273 0.59 +0.9 697,486 - SMa1273 0.59 +0.9 697,486 - SMa1273 0.59 -0.6 697,494 + SMa1273 0.60 -1.4 697,494 + SMa1273 0.60 +0.7 697,495 - SMa1273 0.60 -2.9 697,527 + SMa1273 0.62 +0.1 697,527 + SMa1273 0.62 +0.9 697,611 - SMa1273 0.69 -3.0 697,789 - SMa1273 0.82 -3.0 697,789 - SMa1273 0.82 -0.6 697,821 + SMa1273 0.84 +0.5 697,821 + SMa1273 0.84 -2.6 697,824 + SMa1273 0.84 -2.6 697,824 + SMa1273 0.84 +0.5 697,824 + SMa1273 0.84 +3.7 697,824 + SMa1273 0.84 -0.2 697,825 - SMa1273 0.85 -0.0 697,825 - SMa1273 0.85 -0.4 697,932 + -0.4 697,932 + +1.1 697,933 - -0.4 697,998 + -2.3 698,290 - SMa1276 0.51 -0.7 698,311 + SMa1276 0.56 -0.7 698,406 + SMa1276 0.77 -0.3 698,407 - SMa1276 0.77 +0.3 698,407 - SMa1276 0.77 -0.9 698,409 + SMa1276 0.78 -2.5 698,409 + SMa1276 0.78 -4.1 698,616 + +2.0 698,617 - -1.7 698,617 - +0.4 698,921 + -0.9 698,978 + +0.5 698,979 - +0.8 698,979 - +1.3
Or see this region's nucleotide sequence