Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1239 and SMa1240 overlap by 8 nucleotides SMa1240 and SMa1241 are separated by 7 nucleotides SMa1241 and SMa1243 are separated by 259 nucleotides SMa1243 and SMa1245 are separated by 110 nucleotides
SMa1239: SMa1239 - NapD component of periplasmic nitrate reductase, at 679,677 to 679,964
SMa1239
SMa1240: SMa1240 - NapF ferredoxin component of periplasmic nitrate reductase, at 679,957 to 680,457
SMa1240
SMa1241: SMa1241 - NapE component of periplasmic nitrate reductase, at 680,465 to 680,650
SMa1241
SMa1243: SMa1243 - Azu1 pseudoazurin, at 680,910 to 681,353
SMa1243
SMa1245: SMa1245 - Crp/fnr-like transcriptional regulator, at 681,464 to 682,111
SMa1245
Position (kb) 680
681
682 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 679.937 kb on + strand at 679.942 kb on + strand at 679.942 kb on + strand at 679.942 kb on + strand at 680.055 kb on + strand, within SMa1240 at 680.731 kb on - strand at 680.911 kb on + strand at 681.092 kb on + strand at 681.094 kb on + strand, within SMa1243 at 681.094 kb on + strand, within SMa1243 at 681.095 kb on - strand, within SMa1243 at 681.164 kb on + strand, within SMa1243 at 681.164 kb on + strand, within SMa1243 at 681.165 kb on - strand, within SMa1243 at 681.165 kb on - strand, within SMa1243 at 681.406 kb on - strand at 681.411 kb on - strand at 681.465 kb on + strand at 681.466 kb on - strand at 681.644 kb on + strand, within SMa1245 at 681.645 kb on - strand, within SMa1245 at 681.784 kb on + strand, within SMa1245 at 681.784 kb on + strand, within SMa1245 at 681.784 kb on + strand, within SMa1245 at 681.785 kb on - strand, within SMa1245 at 682.171 kb on + strand at 682.217 kb on + strand at 682.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 679,937 + +0.7 679,942 + -1.9 679,942 + +1.7 679,942 + -0.5 680,055 + SMa1240 0.20 -0.6 680,731 - +4.6 680,911 + -1.5 681,092 + +0.9 681,094 + SMa1243 0.41 -0.1 681,094 + SMa1243 0.41 -1.0 681,095 - SMa1243 0.42 -3.6 681,164 + SMa1243 0.57 +0.3 681,164 + SMa1243 0.57 -3.9 681,165 - SMa1243 0.57 -2.9 681,165 - SMa1243 0.57 -1.1 681,406 - +0.4 681,411 - -1.3 681,465 + +0.3 681,466 - -1.1 681,644 + SMa1245 0.28 +0.7 681,645 - SMa1245 0.28 -0.4 681,784 + SMa1245 0.49 -0.4 681,784 + SMa1245 0.49 -3.0 681,784 + SMa1245 0.49 +4.2 681,785 - SMa1245 0.50 -2.1 682,171 + -1.5 682,217 + +3.1 682,218 - -1.7
Or see this region's nucleotide sequence