Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1231 and SMa1232 are separated by 63 nucleotides SMa1232 and SMa1233 are separated by 3 nucleotides SMa1233 and SMa1236 overlap by 31 nucleotides
SMa1231: SMa1231 - hypothetical protein, at 675,132 to 675,956
SMa1231
SMa1232: SMa1232 - NapC membrane-bound tetraheme cytochrome c subunit, at 676,020 to 676,721
SMa1232
SMa1233: SMa1233 - NapB periplasmic nitrate reductase cytochrome c- type subunit, at 676,725 to 677,228
SMa1233
SMa1236: SMa1236 - nitrate reductase catalytic subunit, at 677,198 to 679,702
SMa1236
Position (kb) 676
677
678 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 675.741 kb on + strand, within SMa1231 at 675.741 kb on + strand, within SMa1231 at 675.741 kb on + strand, within SMa1231 at 675.742 kb on - strand, within SMa1231 at 675.812 kb on - strand, within SMa1231 at 675.865 kb on - strand, within SMa1231 at 675.950 kb on + strand at 675.950 kb on + strand at 675.951 kb on - strand at 675.951 kb on - strand at 675.951 kb on - strand at 675.954 kb on + strand at 676.021 kb on + strand at 676.213 kb on + strand, within SMa1232 at 676.374 kb on + strand, within SMa1232 at 676.375 kb on - strand, within SMa1232 at 676.506 kb on - strand, within SMa1232 at 676.726 kb on + strand at 676.779 kb on - strand, within SMa1233 at 676.879 kb on + strand, within SMa1233 at 676.879 kb on + strand, within SMa1233 at 676.880 kb on - strand, within SMa1233 at 677.312 kb on + strand at 677.446 kb on + strand at 677.476 kb on - strand, within SMa1236 at 677.554 kb on - strand, within SMa1236 at 677.586 kb on + strand, within SMa1236 at 677.628 kb on + strand, within SMa1236 at 677.659 kb on - strand, within SMa1236 at 677.739 kb on + strand, within SMa1236 at 677.749 kb on - strand, within SMa1236 at 677.790 kb on + strand, within SMa1236 at 677.791 kb on - strand, within SMa1236 at 677.791 kb on - strand, within SMa1236 at 677.817 kb on + strand, within SMa1236 at 677.850 kb on + strand, within SMa1236 at 677.850 kb on + strand, within SMa1236 at 677.913 kb on + strand, within SMa1236 at 677.914 kb on - strand, within SMa1236 at 678.064 kb on + strand, within SMa1236 at 678.064 kb on + strand, within SMa1236 at 678.065 kb on - strand, within SMa1236 at 678.156 kb on + strand, within SMa1236 at 678.204 kb on + strand, within SMa1236 at 678.204 kb on + strand, within SMa1236 at 678.205 kb on - strand, within SMa1236 at 678.205 kb on - strand, within SMa1236
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 675,741 + SMa1231 0.74 +0.2 675,741 + SMa1231 0.74 -3.0 675,741 + SMa1231 0.74 -0.1 675,742 - SMa1231 0.74 -2.0 675,812 - SMa1231 0.82 +0.2 675,865 - SMa1231 0.89 +0.5 675,950 + +0.4 675,950 + +0.9 675,951 - -1.3 675,951 - -3.0 675,951 - -0.6 675,954 + +1.0 676,021 + -1.2 676,213 + SMa1232 0.27 -0.6 676,374 + SMa1232 0.50 +0.4 676,375 - SMa1232 0.51 -0.7 676,506 - SMa1232 0.69 -0.9 676,726 + +0.6 676,779 - SMa1233 0.11 -2.6 676,879 + SMa1233 0.31 -1.1 676,879 + SMa1233 0.31 -2.7 676,880 - SMa1233 0.31 +1.5 677,312 + +1.2 677,446 + +1.0 677,476 - SMa1236 0.11 -0.5 677,554 - SMa1236 0.14 -3.5 677,586 + SMa1236 0.15 -0.4 677,628 + SMa1236 0.17 -0.2 677,659 - SMa1236 0.18 -1.0 677,739 + SMa1236 0.22 +0.1 677,749 - SMa1236 0.22 -0.3 677,790 + SMa1236 0.24 +0.2 677,791 - SMa1236 0.24 -2.5 677,791 - SMa1236 0.24 +5.4 677,817 + SMa1236 0.25 +2.1 677,850 + SMa1236 0.26 +2.6 677,850 + SMa1236 0.26 +0.3 677,913 + SMa1236 0.29 +4.6 677,914 - SMa1236 0.29 -0.2 678,064 + SMa1236 0.35 -0.3 678,064 + SMa1236 0.35 -1.1 678,065 - SMa1236 0.35 +2.2 678,156 + SMa1236 0.38 +0.9 678,204 + SMa1236 0.40 +0.7 678,204 + SMa1236 0.40 -1.0 678,205 - SMa1236 0.40 -1.7 678,205 - SMa1236 0.40 -2.0
Or see this region's nucleotide sequence