Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1182 and SMa1183 overlap by 1 nucleotides SMa1183 and SMa1184 overlap by 4 nucleotides SMa1184 and SMa1185 overlap by 4 nucleotides
SMa1182: SMa1182 - nitrous-oxide reductase, at 647,969 to 649,888
SMa1182
SMa1183: SMa1183 - NosD nitrous oxidase accessory protein, at 649,888 to 651,249
SMa1183
SMa1184: SMa1184 - NosF ATPase, at 651,246 to 652,160
SMa1184
SMa1185: SMa1185 - NosY permease, at 652,157 to 652,984
SMa1185
Position (kb) 649
650
651
652 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 648.912 kb on - strand, within SMa1182 at 648.924 kb on + strand, within SMa1182 at 648.956 kb on - strand, within SMa1182 at 649.118 kb on + strand, within SMa1182 at 649.119 kb on - strand, within SMa1182 at 649.139 kb on + strand, within SMa1182 at 649.140 kb on - strand at 649.202 kb on + strand, within SMa1182 at 649.227 kb on + strand, within SMa1182 at 649.228 kb on - strand, within SMa1182 at 649.344 kb on - strand, within SMa1182 at 649.605 kb on - strand, within SMa1182 at 649.605 kb on - strand, within SMa1182 at 649.605 kb on - strand, within SMa1182 at 649.676 kb on + strand, within SMa1182 at 649.677 kb on - strand, within SMa1182 at 649.677 kb on - strand, within SMa1182 at 649.805 kb on + strand at 649.806 kb on - strand at 649.812 kb on - strand at 649.814 kb on + strand at 649.814 kb on + strand at 649.905 kb on - strand at 650.206 kb on + strand, within SMa1183 at 650.297 kb on - strand, within SMa1183 at 650.297 kb on - strand, within SMa1183 at 650.328 kb on - strand, within SMa1183 at 650.384 kb on + strand, within SMa1183 at 650.434 kb on + strand, within SMa1183 at 650.448 kb on + strand, within SMa1183 at 650.450 kb on + strand, within SMa1183 at 650.450 kb on + strand, within SMa1183 at 650.451 kb on - strand, within SMa1183 at 650.522 kb on - strand, within SMa1183 at 650.522 kb on - strand, within SMa1183 at 650.528 kb on - strand, within SMa1183 at 650.528 kb on - strand, within SMa1183 at 650.582 kb on - strand, within SMa1183 at 650.663 kb on - strand, within SMa1183 at 650.663 kb on - strand, within SMa1183 at 650.822 kb on + strand, within SMa1183 at 650.823 kb on - strand, within SMa1183 at 650.830 kb on + strand, within SMa1183 at 650.962 kb on + strand, within SMa1183 at 650.962 kb on + strand, within SMa1183 at 650.962 kb on + strand, within SMa1183 at 650.963 kb on - strand, within SMa1183 at 651.285 kb on + strand at 651.301 kb on + strand at 651.360 kb on - strand, within SMa1184 at 651.360 kb on - strand, within SMa1184 at 651.642 kb on + strand, within SMa1184 at 651.643 kb on - strand, within SMa1184 at 651.643 kb on - strand, within SMa1184 at 651.643 kb on - strand, within SMa1184 at 651.760 kb on - strand, within SMa1184 at 651.769 kb on + strand, within SMa1184 at 651.821 kb on + strand, within SMa1184 at 652.126 kb on - strand at 652.126 kb on - strand at 652.126 kb on - strand at 652.126 kb on - strand at 652.165 kb on + strand at 652.171 kb on + strand at 652.218 kb on + strand at 652.236 kb on + strand at 652.236 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 648,912 - SMa1182 0.49 +2.1 648,924 + SMa1182 0.50 +4.4 648,956 - SMa1182 0.51 -1.2 649,118 + SMa1182 0.60 -2.6 649,119 - SMa1182 0.60 +0.4 649,139 + SMa1182 0.61 +2.4 649,140 - +0.8 649,202 + SMa1182 0.64 +0.3 649,227 + SMa1182 0.66 -1.4 649,228 - SMa1182 0.66 +0.8 649,344 - SMa1182 0.72 -0.0 649,605 - SMa1182 0.85 -1.2 649,605 - SMa1182 0.85 -0.5 649,605 - SMa1182 0.85 +2.2 649,676 + SMa1182 0.89 +0.7 649,677 - SMa1182 0.89 -0.3 649,677 - SMa1182 0.89 -2.1 649,805 + +0.1 649,806 - +1.5 649,812 - -0.7 649,814 + -0.2 649,814 + +0.4 649,905 - -0.2 650,206 + SMa1183 0.23 +0.3 650,297 - SMa1183 0.30 +0.4 650,297 - SMa1183 0.30 +0.2 650,328 - SMa1183 0.32 -1.5 650,384 + SMa1183 0.36 +1.3 650,434 + SMa1183 0.40 +0.9 650,448 + SMa1183 0.41 -0.7 650,450 + SMa1183 0.41 +1.3 650,450 + SMa1183 0.41 -3.6 650,451 - SMa1183 0.41 +1.5 650,522 - SMa1183 0.47 +1.2 650,522 - SMa1183 0.47 +0.5 650,528 - SMa1183 0.47 +0.6 650,528 - SMa1183 0.47 -0.6 650,582 - SMa1183 0.51 -0.5 650,663 - SMa1183 0.57 +1.6 650,663 - SMa1183 0.57 +0.4 650,822 + SMa1183 0.69 +0.3 650,823 - SMa1183 0.69 +0.7 650,830 + SMa1183 0.69 +0.6 650,962 + SMa1183 0.79 +0.3 650,962 + SMa1183 0.79 +0.8 650,962 + SMa1183 0.79 -1.7 650,963 - SMa1183 0.79 -0.2 651,285 + -2.3 651,301 + -0.2 651,360 - SMa1184 0.12 -3.2 651,360 - SMa1184 0.12 +0.5 651,642 + SMa1184 0.43 -0.5 651,643 - SMa1184 0.43 +1.3 651,643 - SMa1184 0.43 -0.4 651,643 - SMa1184 0.43 +3.0 651,760 - SMa1184 0.56 +1.3 651,769 + SMa1184 0.57 -0.3 651,821 + SMa1184 0.63 -0.7 652,126 - +0.6 652,126 - +0.3 652,126 - -1.5 652,126 - -0.7 652,165 + -2.0 652,171 + -2.1 652,218 + -0.6 652,236 + +5.1 652,236 + -0.1
Or see this region's nucleotide sequence