Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1087 and SMa1089 overlap by 4 nucleotides SMa1089 and SMa1091 overlap by 4 nucleotides SMa1091 and SMa1092 are separated by 223 nucleotides SMa1092 and SMa1093 are separated by 253 nucleotides SMa1093 and SMa1095 are separated by 187 nucleotides
SMa1087: SMa1087 - cation transport P-type ATPase, at 594,396 to 596,597
SMa1087
SMa1089: SMa1089 - hypothetical protein, at 596,594 to 596,797
SMa1089
SMa1091: SMa1091 - hypothetical protein, at 596,794 to 597,297
SMa1091
SMa1092: SMa1092 - hypothetical protein, at 597,521 to 598,030
SMa1092
SMa1093: SMa1093 - hypothetical protein, at 598,284 to 598,616
SMa1093
SMa1095: SMa1095 - hypothetical protein, at 598,804 to 599,112
SMa1095
Position (kb) 597
598
599 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 596.594 kb on - strand at 596.594 kb on - strand at 596.789 kb on - strand at 596.801 kb on - strand at 596.861 kb on - strand, within SMa1091 at 596.935 kb on + strand, within SMa1091 at 596.996 kb on + strand, within SMa1091 at 596.996 kb on + strand, within SMa1091 at 596.996 kb on + strand, within SMa1091 at 596.996 kb on + strand, within SMa1091 at 596.997 kb on - strand, within SMa1091 at 596.997 kb on - strand, within SMa1091 at 597.059 kb on + strand, within SMa1091 at 597.169 kb on + strand, within SMa1091 at 597.227 kb on - strand, within SMa1091 at 597.295 kb on + strand at 597.295 kb on + strand at 597.421 kb on + strand at 597.422 kb on - strand at 597.422 kb on - strand at 597.445 kb on - strand at 597.522 kb on + strand at 597.523 kb on - strand at 597.523 kb on - strand at 597.523 kb on - strand at 597.523 kb on - strand at 597.577 kb on + strand, within SMa1092 at 597.654 kb on + strand, within SMa1092 at 597.954 kb on - strand at 597.989 kb on - strand at 597.989 kb on - strand at 597.989 kb on - strand at 598.064 kb on + strand at 598.065 kb on - strand at 598.065 kb on - strand at 598.126 kb on - strand at 598.270 kb on + strand at 598.270 kb on + strand at 598.271 kb on - strand at 598.271 kb on - strand at 598.361 kb on + strand, within SMa1093 at 598.382 kb on - strand, within SMa1093 at 598.551 kb on + strand, within SMa1093 at 598.576 kb on - strand, within SMa1093 at 598.576 kb on - strand, within SMa1093 at 598.598 kb on + strand at 598.748 kb on + strand at 598.834 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 596,594 - -0.1 596,594 - -1.9 596,789 - +0.4 596,801 - -0.9 596,861 - SMa1091 0.13 +1.1 596,935 + SMa1091 0.28 -1.2 596,996 + SMa1091 0.40 -0.9 596,996 + SMa1091 0.40 -3.3 596,996 + SMa1091 0.40 +1.8 596,996 + SMa1091 0.40 -2.2 596,997 - SMa1091 0.40 -1.8 596,997 - SMa1091 0.40 +1.0 597,059 + SMa1091 0.53 -5.0 597,169 + SMa1091 0.74 +0.0 597,227 - SMa1091 0.86 -1.2 597,295 + -1.2 597,295 + -2.5 597,421 + +0.8 597,422 - -0.7 597,422 - +0.8 597,445 - -0.1 597,522 + -1.8 597,523 - -0.3 597,523 - +0.8 597,523 - +1.1 597,523 - +0.9 597,577 + SMa1092 0.11 -0.7 597,654 + SMa1092 0.26 -1.0 597,954 - +2.8 597,989 - -2.0 597,989 - +0.2 597,989 - +0.5 598,064 + -1.2 598,065 - -0.4 598,065 - +0.6 598,126 - +1.0 598,270 + -1.5 598,270 + +0.6 598,271 - +0.2 598,271 - +0.4 598,361 + SMa1093 0.23 +0.7 598,382 - SMa1093 0.29 +0.8 598,551 + SMa1093 0.80 -2.8 598,576 - SMa1093 0.88 +0.6 598,576 - SMa1093 0.88 +0.8 598,598 + +1.2 598,748 + -3.4 598,834 - -2.6
Or see this region's nucleotide sequence