Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0873 and SMa0874 are separated by 87 nucleotides SMa0874 and SMa0875 are separated by 6 nucleotides SMa0875 and SMa0876 are separated by 3 nucleotides
SMa0873: SMa0873 - nitrogenase molybdenum-cofactor biosynthesis protein NifN, at 483,756 to 485,081
SMa0873
SMa0874: SMa0874 - NodN dehydratase, at 485,169 to 485,654
SMa0874
SMa0875: SMa0875 - NolG efflux transporter, at 485,661 to 488,858
SMa0875
SMa0876: SMa0876 - NolF secretion protein, at 488,862 to 489,965
SMa0876
Position (kb) 485
486
487
488
489 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 484.728 kb on + strand, within SMa0873 at 484.740 kb on - strand, within SMa0873 at 484.828 kb on + strand, within SMa0873 at 484.936 kb on + strand, within SMa0873 at 485.283 kb on + strand, within SMa0874 at 485.287 kb on - strand, within SMa0874 at 485.302 kb on - strand, within SMa0874 at 485.302 kb on - strand, within SMa0874 at 485.520 kb on + strand, within SMa0874 at 485.521 kb on - strand, within SMa0874 at 485.666 kb on + strand at 485.785 kb on - strand at 485.809 kb on + strand at 485.810 kb on - strand at 485.810 kb on - strand at 485.856 kb on + strand at 486.116 kb on + strand, within SMa0875 at 486.116 kb on + strand, within SMa0875 at 486.116 kb on + strand, within SMa0875 at 486.117 kb on - strand, within SMa0875 at 486.117 kb on - strand, within SMa0875 at 486.117 kb on - strand, within SMa0875 at 486.134 kb on + strand, within SMa0875 at 486.236 kb on - strand, within SMa0875 at 486.525 kb on - strand, within SMa0875 at 486.637 kb on - strand, within SMa0875 at 486.637 kb on - strand, within SMa0875 at 487.069 kb on + strand, within SMa0875 at 487.069 kb on + strand, within SMa0875 at 487.120 kb on + strand, within SMa0875 at 487.120 kb on + strand, within SMa0875 at 487.121 kb on - strand, within SMa0875 at 487.240 kb on - strand, within SMa0875 at 487.248 kb on - strand, within SMa0875 at 487.422 kb on - strand, within SMa0875 at 487.458 kb on + strand, within SMa0875 at 487.618 kb on - strand, within SMa0875 at 487.618 kb on - strand, within SMa0875 at 487.641 kb on + strand, within SMa0875 at 487.766 kb on + strand, within SMa0875 at 488.039 kb on - strand, within SMa0875 at 488.039 kb on - strand, within SMa0875 at 488.201 kb on + strand, within SMa0875 at 488.201 kb on + strand, within SMa0875 at 488.272 kb on + strand, within SMa0875 at 488.272 kb on + strand, within SMa0875 at 488.357 kb on + strand, within SMa0875 at 488.357 kb on + strand, within SMa0875 at 488.358 kb on - strand, within SMa0875 at 488.358 kb on - strand, within SMa0875 at 488.358 kb on - strand, within SMa0875 at 488.605 kb on + strand at 488.707 kb on + strand at 488.707 kb on + strand at 488.708 kb on - strand at 488.708 kb on - strand at 488.774 kb on - strand at 488.816 kb on + strand at 488.843 kb on + strand at 488.844 kb on - strand at 489.105 kb on + strand, within SMa0876 at 489.106 kb on - strand, within SMa0876 at 489.252 kb on + strand, within SMa0876 at 489.264 kb on + strand, within SMa0876 at 489.483 kb on + strand, within SMa0876 at 489.484 kb on - strand, within SMa0876 at 489.718 kb on - strand, within SMa0876 at 489.718 kb on - strand, within SMa0876 at 489.755 kb on + strand, within SMa0876 at 489.755 kb on + strand at 489.755 kb on + strand, within SMa0876 at 489.756 kb on - strand, within SMa0876 at 489.756 kb on - strand, within SMa0876 at 489.774 kb on + strand, within SMa0876 at 489.786 kb on + strand, within SMa0876 at 489.788 kb on + strand, within SMa0876 at 489.789 kb on - strand, within SMa0876 at 489.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 484,728 + SMa0873 0.73 +1.8 484,740 - SMa0873 0.74 -3.3 484,828 + SMa0873 0.81 +0.0 484,936 + SMa0873 0.89 +1.5 485,283 + SMa0874 0.23 +3.2 485,287 - SMa0874 0.24 -2.0 485,302 - SMa0874 0.27 -0.2 485,302 - SMa0874 0.27 +0.8 485,520 + SMa0874 0.72 +3.6 485,521 - SMa0874 0.72 +1.5 485,666 + -2.0 485,785 - +0.2 485,809 + +2.7 485,810 - -1.0 485,810 - -0.4 485,856 + +0.3 486,116 + SMa0875 0.14 +0.4 486,116 + SMa0875 0.14 +0.4 486,116 + SMa0875 0.14 -0.3 486,117 - SMa0875 0.14 -0.8 486,117 - SMa0875 0.14 +0.6 486,117 - SMa0875 0.14 +2.0 486,134 + SMa0875 0.15 -0.1 486,236 - SMa0875 0.18 -0.2 486,525 - SMa0875 0.27 -0.4 486,637 - SMa0875 0.31 +1.7 486,637 - SMa0875 0.31 -0.4 487,069 + SMa0875 0.44 +0.1 487,069 + SMa0875 0.44 +0.0 487,120 + SMa0875 0.46 +0.4 487,120 + SMa0875 0.46 -0.5 487,121 - SMa0875 0.46 +0.8 487,240 - SMa0875 0.49 -0.5 487,248 - SMa0875 0.50 +1.4 487,422 - SMa0875 0.55 -1.7 487,458 + SMa0875 0.56 +2.8 487,618 - SMa0875 0.61 +1.1 487,618 - SMa0875 0.61 -0.8 487,641 + SMa0875 0.62 -2.4 487,766 + SMa0875 0.66 -0.4 488,039 - SMa0875 0.74 -0.2 488,039 - SMa0875 0.74 +0.5 488,201 + SMa0875 0.79 +0.4 488,201 + SMa0875 0.79 -2.8 488,272 + SMa0875 0.82 +0.9 488,272 + SMa0875 0.82 +1.6 488,357 + SMa0875 0.84 +2.1 488,357 + SMa0875 0.84 +2.2 488,358 - SMa0875 0.84 +0.1 488,358 - SMa0875 0.84 -2.0 488,358 - SMa0875 0.84 +0.8 488,605 + -1.7 488,707 + +0.6 488,707 + +0.6 488,708 - +0.1 488,708 - +0.2 488,774 - +0.8 488,816 + -4.0 488,843 + +1.0 488,844 - -0.2 489,105 + SMa0876 0.22 +0.4 489,106 - SMa0876 0.22 -0.3 489,252 + SMa0876 0.35 +1.8 489,264 + SMa0876 0.36 -2.2 489,483 + SMa0876 0.56 +3.6 489,484 - SMa0876 0.56 +1.8 489,718 - SMa0876 0.78 +3.6 489,718 - SMa0876 0.78 +0.2 489,755 + SMa0876 0.81 +1.0 489,755 + +0.8 489,755 + SMa0876 0.81 -1.8 489,756 - SMa0876 0.81 +0.6 489,756 - SMa0876 0.81 -0.5 489,774 + SMa0876 0.83 -0.4 489,786 + SMa0876 0.84 +1.9 489,788 + SMa0876 0.84 -2.3 489,789 - SMa0876 0.84 +0.4 489,858 - +0.8
Or see this region's nucleotide sequence