Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0868 and SMa0869 overlap by 4 nucleotides SMa0869 and SMa0870 are separated by 265 nucleotides SMa0870 and SMa0872 are separated by 687 nucleotides
SMa0868: SMa0868 - NodB chitooligosaccharide deacetylase, at 480,304 to 480,957
SMa0868
SMa0869: SMa0869 - acyltransferase, at 480,954 to 481,544
SMa0869
SMa0870: SMa0870 - NodD1 nod-box dependent transcriptional activator, at 481,810 to 482,736
SMa0870
SMa0872: SMa0872 - hypothetical protein, at 483,424 to 483,756
SMa0872
Position (kb)
481
482
483 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 480.822 kb on - strand, within SMa0868 at 480.889 kb on + strand, within SMa0868 at 480.889 kb on + strand, within SMa0868 at 480.891 kb on + strand, within SMa0868 at 480.891 kb on + strand, within SMa0868 at 480.892 kb on - strand at 480.937 kb on + strand at 480.938 kb on - strand at 480.942 kb on - strand at 480.958 kb on - strand at 481.138 kb on + strand, within SMa0869 at 481.273 kb on + strand, within SMa0869 at 481.273 kb on + strand, within SMa0869 at 481.276 kb on - strand, within SMa0869 at 481.316 kb on + strand, within SMa0869 at 481.316 kb on + strand, within SMa0869 at 481.317 kb on - strand, within SMa0869 at 481.518 kb on + strand at 481.519 kb on - strand at 481.546 kb on + strand at 481.547 kb on - strand at 481.547 kb on - strand at 481.547 kb on - strand at 481.547 kb on - strand at 481.654 kb on - strand at 481.740 kb on + strand at 482.039 kb on + strand, within SMa0870 at 482.079 kb on - strand, within SMa0870 at 482.132 kb on + strand, within SMa0870 at 482.133 kb on - strand, within SMa0870 at 482.378 kb on - strand, within SMa0870 at 482.378 kb on - strand, within SMa0870 at 482.687 kb on + strand at 482.735 kb on - strand at 482.754 kb on - strand at 482.868 kb on - strand at 482.869 kb on + strand at 483.048 kb on + strand at 483.069 kb on + strand at 483.070 kb on - strand at 483.085 kb on - strand at 483.087 kb on - strand at 483.175 kb on - strand at 483.283 kb on + strand at 483.401 kb on - strand at 483.489 kb on + strand, within SMa0872 at 483.647 kb on + strand, within SMa0872 at 483.648 kb on - strand, within SMa0872 at 483.654 kb on - strand, within SMa0872 at 483.654 kb on - strand, within SMa0872
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 480,822 - SMa0868 0.79 +0.8 480,889 + SMa0868 0.89 -1.5 480,889 + SMa0868 0.89 +0.0 480,891 + SMa0868 0.90 -1.0 480,891 + SMa0868 0.90 +2.8 480,892 - +0.8 480,937 + -0.7 480,938 - +0.6 480,942 - -2.9 480,958 - +0.8 481,138 + SMa0869 0.31 +0.2 481,273 + SMa0869 0.54 -2.4 481,273 + SMa0869 0.54 -1.0 481,276 - SMa0869 0.54 -2.0 481,316 + SMa0869 0.61 +1.7 481,316 + SMa0869 0.61 +0.0 481,317 - SMa0869 0.61 -0.5 481,518 + -2.7 481,519 - +0.2 481,546 + -0.8 481,547 - +1.1 481,547 - +0.4 481,547 - -2.0 481,547 - -1.5 481,654 - -2.8 481,740 + +0.1 482,039 + SMa0870 0.25 -2.9 482,079 - SMa0870 0.29 +0.9 482,132 + SMa0870 0.35 -0.2 482,133 - SMa0870 0.35 +1.2 482,378 - SMa0870 0.61 +1.0 482,378 - SMa0870 0.61 +3.4 482,687 + -0.2 482,735 - +1.6 482,754 - +2.8 482,868 - -1.5 482,869 + +4.4 483,048 + -0.3 483,069 + +0.2 483,070 - +0.7 483,085 - -1.5 483,087 - +0.0 483,175 - -0.9 483,283 + -0.6 483,401 - -1.7 483,489 + SMa0872 0.20 -1.6 483,647 + SMa0872 0.67 -1.0 483,648 - SMa0872 0.67 +1.2 483,654 - SMa0872 0.69 +0.4 483,654 - SMa0872 0.69 -3.0
Or see this region's nucleotide sequence