Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0824 and SMa0825 are separated by 230 nucleotides
SMa0824: SMa0824 - hypothetical protein, at 452,858 to 453,325
SMa0824
SMa0825: SMa0825 - nitrogenase reductase, at 453,556 to 454,449
SMa0825
Position (kb) 452
453
454 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 451.860 kb on - strand at 451.880 kb on + strand at 451.881 kb on - strand at 451.962 kb on + strand at 452.230 kb on + strand at 452.301 kb on + strand at 452.302 kb on - strand at 452.302 kb on - strand at 452.302 kb on - strand at 452.345 kb on + strand at 452.346 kb on - strand at 452.361 kb on + strand at 452.362 kb on - strand at 452.415 kb on + strand at 452.461 kb on - strand at 452.479 kb on + strand at 452.480 kb on - strand at 452.484 kb on - strand at 452.714 kb on + strand at 452.763 kb on - strand at 452.796 kb on + strand at 452.837 kb on + strand at 453.153 kb on + strand, within SMa0824 at 453.153 kb on + strand, within SMa0824 at 453.153 kb on + strand, within SMa0824 at 453.153 kb on + strand, within SMa0824 at 453.154 kb on - strand, within SMa0824 at 453.159 kb on + strand, within SMa0824 at 453.159 kb on + strand, within SMa0824 at 453.160 kb on - strand, within SMa0824 at 453.418 kb on + strand at 453.418 kb on + strand at 453.419 kb on - strand at 453.531 kb on + strand at 453.536 kb on + strand at 453.537 kb on - strand at 453.583 kb on + strand at 453.584 kb on - strand at 453.624 kb on + strand at 453.731 kb on + strand, within SMa0825 at 453.867 kb on + strand, within SMa0825 at 453.904 kb on + strand, within SMa0825 at 453.928 kb on + strand, within SMa0825 at 453.947 kb on + strand, within SMa0825 at 453.948 kb on - strand, within SMa0825 at 453.973 kb on - strand, within SMa0825 at 454.034 kb on - strand, within SMa0825 at 454.059 kb on + strand, within SMa0825 at 454.261 kb on - strand, within SMa0825 at 454.261 kb on - strand, within SMa0825
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 451,860 - +0.7 451,880 + -0.8 451,881 - +1.8 451,962 + +0.8 452,230 + -2.2 452,301 + -2.3 452,302 - -0.3 452,302 - +0.1 452,302 - -1.2 452,345 + -1.5 452,346 - +0.8 452,361 + -3.5 452,362 - +2.0 452,415 + +0.3 452,461 - -0.0 452,479 + +1.0 452,480 - -1.2 452,484 - -2.2 452,714 + -1.8 452,763 - +0.7 452,796 + +3.1 452,837 + -1.5 453,153 + SMa0824 0.63 -2.9 453,153 + SMa0824 0.63 -0.8 453,153 + SMa0824 0.63 -2.2 453,153 + SMa0824 0.63 -1.4 453,154 - SMa0824 0.63 -2.5 453,159 + SMa0824 0.64 -1.0 453,159 + SMa0824 0.64 -0.4 453,160 - SMa0824 0.65 +0.5 453,418 + +0.8 453,418 + +0.9 453,419 - +1.4 453,531 + +2.4 453,536 + -0.7 453,537 - +1.8 453,583 + +1.2 453,584 - -0.9 453,624 + +1.2 453,731 + SMa0825 0.20 -3.7 453,867 + SMa0825 0.35 -2.4 453,904 + SMa0825 0.39 -0.1 453,928 + SMa0825 0.42 +2.5 453,947 + SMa0825 0.44 -0.1 453,948 - SMa0825 0.44 +1.1 453,973 - SMa0825 0.47 -1.8 454,034 - SMa0825 0.53 +0.6 454,059 + SMa0825 0.56 -0.3 454,261 - SMa0825 0.79 +0.2 454,261 - SMa0825 0.79 -0.5
Or see this region's nucleotide sequence