Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0796 and SMa0797 are separated by 5 nucleotides SMa0797 and SMa0799 are separated by 37 nucleotides SMa0799 and SMa0800 are separated by 92 nucleotides SMa0800 and SMa0802 are separated by 1 nucleotides
SMa0796: SMa0796 - hypothetical protein, at 434,028 to 435,491
SMa0796
SMa0797: SMa0797 - hypothetical protein, at 435,497 to 435,823
SMa0797
SMa0799: SMa0799 - ABC transporter substrate-binding protein, at 435,861 to 436,871
SMa0799
SMa0800: SMa0800 - ABC transporter permease, at 436,964 to 437,839
SMa0800
SMa0802: SMa0802 - ABC transporter permease, at 437,841 to 438,641
SMa0802
Position (kb) 435
436
437 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 435.105 kb on + strand, within SMa0796 at 435.105 kb on + strand, within SMa0796 at 435.105 kb on + strand, within SMa0796 at 435.329 kb on + strand, within SMa0796 at 435.330 kb on - strand, within SMa0796 at 435.330 kb on - strand, within SMa0796 at 435.333 kb on + strand, within SMa0796 at 435.334 kb on - strand, within SMa0796 at 435.420 kb on + strand at 435.519 kb on - strand at 435.519 kb on - strand at 435.545 kb on - strand, within SMa0797 at 435.655 kb on + strand, within SMa0797 at 435.656 kb on - strand, within SMa0797 at 435.665 kb on + strand, within SMa0797 at 435.772 kb on + strand, within SMa0797 at 435.822 kb on - strand at 435.866 kb on + strand at 435.866 kb on + strand at 435.872 kb on - strand at 435.872 kb on - strand at 435.905 kb on + strand at 435.905 kb on + strand at 435.905 kb on + strand at 435.906 kb on - strand at 435.906 kb on - strand at 435.906 kb on - strand at 436.062 kb on - strand, within SMa0799 at 436.062 kb on - strand, within SMa0799 at 436.062 kb on - strand, within SMa0799 at 436.062 kb on - strand, within SMa0799 at 436.207 kb on - strand, within SMa0799 at 436.225 kb on - strand, within SMa0799 at 436.227 kb on + strand, within SMa0799 at 436.227 kb on + strand, within SMa0799 at 436.227 kb on + strand, within SMa0799 at 436.228 kb on - strand, within SMa0799 at 436.228 kb on - strand, within SMa0799 at 436.329 kb on + strand, within SMa0799 at 436.330 kb on - strand, within SMa0799 at 436.381 kb on + strand, within SMa0799 at 436.443 kb on - strand, within SMa0799 at 436.517 kb on + strand, within SMa0799 at 436.657 kb on + strand, within SMa0799 at 436.968 kb on + strand at 436.969 kb on - strand at 437.000 kb on + strand at 437.056 kb on - strand, within SMa0800 at 437.101 kb on - strand, within SMa0800 at 437.142 kb on - strand, within SMa0800 at 437.142 kb on - strand, within SMa0800 at 437.340 kb on - strand, within SMa0800 at 437.462 kb on + strand, within SMa0800 at 437.462 kb on + strand, within SMa0800 at 437.462 kb on + strand, within SMa0800 at 437.463 kb on - strand, within SMa0800 at 437.463 kb on - strand, within SMa0800 at 437.463 kb on - strand, within SMa0800 at 437.463 kb on - strand, within SMa0800 at 437.463 kb on - strand, within SMa0800 at 437.463 kb on - strand, within SMa0800 at 437.495 kb on + strand, within SMa0800 at 437.495 kb on + strand, within SMa0800 at 437.496 kb on - strand, within SMa0800 at 437.496 kb on - strand, within SMa0800 at 437.678 kb on - strand, within SMa0800 at 437.789 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 435,105 + SMa0796 0.74 -1.5 435,105 + SMa0796 0.74 +0.5 435,105 + SMa0796 0.74 +0.3 435,329 + SMa0796 0.89 +0.2 435,330 - SMa0796 0.89 -1.2 435,330 - SMa0796 0.89 -1.8 435,333 + SMa0796 0.89 +0.8 435,334 - SMa0796 0.89 -1.0 435,420 + -0.9 435,519 - +2.8 435,519 - -2.8 435,545 - SMa0797 0.15 -2.0 435,655 + SMa0797 0.48 +0.1 435,656 - SMa0797 0.49 -0.5 435,665 + SMa0797 0.51 -2.5 435,772 + SMa0797 0.84 +0.8 435,822 - -2.7 435,866 + +1.8 435,866 + -0.2 435,872 - -0.8 435,872 - -3.1 435,905 + -0.5 435,905 + -0.1 435,905 + -2.5 435,906 - +0.8 435,906 - +0.1 435,906 - +0.6 436,062 - SMa0799 0.20 +0.0 436,062 - SMa0799 0.20 +0.4 436,062 - SMa0799 0.20 +0.8 436,062 - SMa0799 0.20 +0.3 436,207 - SMa0799 0.34 -0.5 436,225 - SMa0799 0.36 -1.9 436,227 + SMa0799 0.36 -1.5 436,227 + SMa0799 0.36 -0.4 436,227 + SMa0799 0.36 -1.2 436,228 - SMa0799 0.36 -0.4 436,228 - SMa0799 0.36 +1.1 436,329 + SMa0799 0.46 -0.4 436,330 - SMa0799 0.46 -0.3 436,381 + SMa0799 0.51 +0.8 436,443 - SMa0799 0.58 -1.1 436,517 + SMa0799 0.65 +0.6 436,657 + SMa0799 0.79 -1.3 436,968 + +0.8 436,969 - -3.7 437,000 + +0.1 437,056 - SMa0800 0.11 +2.0 437,101 - SMa0800 0.16 +2.1 437,142 - SMa0800 0.20 +3.0 437,142 - SMa0800 0.20 +1.1 437,340 - SMa0800 0.43 +0.5 437,462 + SMa0800 0.57 -1.7 437,462 + SMa0800 0.57 -0.6 437,462 + SMa0800 0.57 -1.5 437,463 - SMa0800 0.57 +0.8 437,463 - SMa0800 0.57 -1.8 437,463 - SMa0800 0.57 -2.7 437,463 - SMa0800 0.57 -1.2 437,463 - SMa0800 0.57 -0.5 437,463 - SMa0800 0.57 -0.2 437,495 + SMa0800 0.61 +0.8 437,495 + SMa0800 0.61 -1.0 437,496 - SMa0800 0.61 +1.8 437,496 - SMa0800 0.61 +1.5 437,678 - SMa0800 0.82 -1.1 437,789 + -3.8
Or see this region's nucleotide sequence