Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0612 and SMa0615 are separated by 4 nucleotides SMa0615 and SMa0616 are separated by 22 nucleotides SMa0616 and SMa0617 overlap by 10 nucleotides SMa0617 and SMa0620 are separated by 218 nucleotides
SMa0612: SMa0612 - cbb3-type cytochrome c oxidase subunit I, at 326,972 to 328,627
SMa0612
SMa0615: SMa0615 - cbb3-type cytochrome c oxidase subunit II, at 328,632 to 329,384
SMa0615
SMa0616: SMa0616 - FixQ3 nitrogen fixation protein, at 329,407 to 329,577
SMa0616
SMa0617: SMa0617 - FixP3 Diheme c-type cytochrome, at 329,568 to 330,449
SMa0617
SMa0620: SMa0620 - hypothetical protein, at 330,668 to 331,474
SMa0620
Position (kb) 329
330
331 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 328.815 kb on + strand, within SMa0615 at 328.816 kb on - strand, within SMa0615 at 328.833 kb on + strand, within SMa0615 at 328.834 kb on - strand, within SMa0615 at 328.917 kb on + strand, within SMa0615 at 328.972 kb on + strand, within SMa0615 at 328.973 kb on - strand, within SMa0615 at 328.984 kb on - strand, within SMa0615 at 328.984 kb on - strand, within SMa0615 at 329.002 kb on + strand, within SMa0615 at 329.002 kb on + strand, within SMa0615 at 329.055 kb on + strand, within SMa0615 at 329.136 kb on + strand, within SMa0615 at 329.337 kb on + strand at 329.338 kb on - strand at 329.338 kb on - strand at 329.338 kb on - strand at 329.338 kb on - strand at 329.745 kb on + strand, within SMa0617 at 329.745 kb on + strand, within SMa0617 at 329.745 kb on + strand, within SMa0617 at 329.746 kb on - strand, within SMa0617 at 329.747 kb on + strand, within SMa0617 at 329.747 kb on + strand, within SMa0617 at 329.843 kb on + strand, within SMa0617 at 329.844 kb on - strand, within SMa0617 at 329.863 kb on + strand, within SMa0617 at 329.863 kb on + strand, within SMa0617 at 329.863 kb on + strand, within SMa0617 at 329.864 kb on - strand, within SMa0617 at 329.970 kb on + strand, within SMa0617 at 329.970 kb on + strand, within SMa0617 at 329.970 kb on + strand, within SMa0617 at 330.141 kb on + strand, within SMa0617 at 330.142 kb on - strand, within SMa0617 at 330.142 kb on - strand, within SMa0617 at 330.168 kb on + strand, within SMa0617 at 330.307 kb on - strand, within SMa0617 at 330.327 kb on - strand, within SMa0617 at 330.362 kb on + strand at 330.362 kb on + strand at 330.363 kb on - strand at 330.363 kb on - strand at 330.417 kb on + strand at 330.417 kb on + strand at 330.546 kb on - strand at 330.546 kb on - strand at 330.667 kb on + strand at 330.667 kb on + strand at 330.667 kb on + strand at 330.668 kb on - strand at 330.717 kb on + strand at 330.717 kb on + strand at 330.717 kb on + strand at 330.810 kb on - strand, within SMa0620 at 330.831 kb on - strand, within SMa0620 at 330.831 kb on - strand, within SMa0620 at 330.925 kb on + strand, within SMa0620 at 330.925 kb on + strand, within SMa0620 at 330.960 kb on - strand, within SMa0620 at 331.109 kb on + strand, within SMa0620 at 331.110 kb on - strand, within SMa0620 at 331.153 kb on + strand, within SMa0620 at 331.371 kb on + strand, within SMa0620
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 328,815 + SMa0615 0.24 +1.0 328,816 - SMa0615 0.24 +4.0 328,833 + SMa0615 0.27 -0.5 328,834 - SMa0615 0.27 -1.8 328,917 + SMa0615 0.38 +2.4 328,972 + SMa0615 0.45 -1.7 328,973 - SMa0615 0.45 -0.5 328,984 - SMa0615 0.47 -0.1 328,984 - SMa0615 0.47 -0.1 329,002 + SMa0615 0.49 +1.0 329,002 + SMa0615 0.49 +0.0 329,055 + SMa0615 0.56 +0.2 329,136 + SMa0615 0.67 +0.0 329,337 + +0.2 329,338 - -2.0 329,338 - -2.2 329,338 - -0.5 329,338 - +0.1 329,745 + SMa0617 0.20 +1.5 329,745 + SMa0617 0.20 +0.4 329,745 + SMa0617 0.20 -1.3 329,746 - SMa0617 0.20 +0.9 329,747 + SMa0617 0.20 +1.8 329,747 + SMa0617 0.20 +0.8 329,843 + SMa0617 0.31 +0.5 329,844 - SMa0617 0.31 +2.4 329,863 + SMa0617 0.33 -0.1 329,863 + SMa0617 0.33 -1.0 329,863 + SMa0617 0.33 -0.6 329,864 - SMa0617 0.34 +0.4 329,970 + SMa0617 0.46 -0.7 329,970 + SMa0617 0.46 -2.3 329,970 + SMa0617 0.46 +1.7 330,141 + SMa0617 0.65 -2.0 330,142 - SMa0617 0.65 +3.2 330,142 - SMa0617 0.65 -0.0 330,168 + SMa0617 0.68 +1.4 330,307 - SMa0617 0.84 -1.8 330,327 - SMa0617 0.86 +1.4 330,362 + +0.2 330,362 + +2.8 330,363 - +1.0 330,363 - +1.8 330,417 + -0.5 330,417 + +0.8 330,546 - -2.3 330,546 - +3.4 330,667 + -1.7 330,667 + +0.7 330,667 + +0.3 330,668 - +2.8 330,717 + -0.2 330,717 + +1.5 330,717 + -0.7 330,810 - SMa0620 0.18 -0.7 330,831 - SMa0620 0.20 -1.0 330,831 - SMa0620 0.20 +0.3 330,925 + SMa0620 0.32 +0.6 330,925 + SMa0620 0.32 +0.5 330,960 - SMa0620 0.36 -0.2 331,109 + SMa0620 0.55 -0.7 331,110 - SMa0620 0.55 +0.8 331,153 + SMa0620 0.60 +1.6 331,371 + SMa0620 0.87 -4.3
Or see this region's nucleotide sequence