Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0559 and SMa0561 are separated by 143 nucleotides SMa0561 and SMa0563 are separated by 10 nucleotides SMa0563 and SMa0564 are separated by 65 nucleotides
SMa0559: SMa0559 - 4-carboxymuconolactone decarboxylase, at 297,048 to 297,791
SMa0559
SMa0561: SMa0561 - hypothetical protein, at 297,935 to 298,318
SMa0561
SMa0563: SMa0563 - aldehyde or keto oxidase, at 298,329 to 299,318
SMa0563
SMa0564: SMa0564 - dehydrogenase, at 299,384 to 300,487
SMa0564
Position (kb) 298
299
300 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 297.336 kb on + strand, within SMa0559 at 297.337 kb on - strand, within SMa0559 at 297.339 kb on + strand, within SMa0559 at 297.556 kb on + strand, within SMa0559 at 297.556 kb on + strand, within SMa0559 at 297.557 kb on - strand, within SMa0559 at 297.557 kb on - strand, within SMa0559 at 297.557 kb on - strand, within SMa0559 at 297.852 kb on + strand at 297.852 kb on + strand at 297.853 kb on - strand at 297.853 kb on - strand at 297.951 kb on + strand at 297.951 kb on + strand at 297.951 kb on + strand at 297.952 kb on - strand at 297.952 kb on - strand at 297.952 kb on - strand at 298.031 kb on + strand, within SMa0561 at 298.031 kb on + strand, within SMa0561 at 298.032 kb on - strand, within SMa0561 at 298.080 kb on - strand, within SMa0561 at 298.080 kb on - strand, within SMa0561 at 298.080 kb on - strand, within SMa0561 at 298.116 kb on - strand, within SMa0561 at 298.239 kb on - strand, within SMa0561 at 298.296 kb on + strand at 298.392 kb on + strand at 298.491 kb on + strand, within SMa0563 at 298.491 kb on + strand, within SMa0563 at 298.491 kb on + strand, within SMa0563 at 298.492 kb on - strand, within SMa0563 at 298.492 kb on - strand, within SMa0563 at 298.492 kb on - strand, within SMa0563 at 298.682 kb on + strand, within SMa0563 at 298.861 kb on - strand, within SMa0563 at 299.093 kb on + strand, within SMa0563 at 299.206 kb on + strand, within SMa0563 at 299.206 kb on + strand, within SMa0563 at 299.379 kb on + strand at 299.472 kb on + strand at 299.473 kb on - strand at 299.568 kb on - strand, within SMa0564 at 299.765 kb on + strand at 299.766 kb on - strand, within SMa0564 at 299.772 kb on - strand, within SMa0564 at 299.772 kb on - strand, within SMa0564 at 299.877 kb on + strand, within SMa0564 at 299.878 kb on - strand, within SMa0564 at 299.879 kb on + strand, within SMa0564 at 299.879 kb on + strand, within SMa0564 at 299.880 kb on - strand, within SMa0564 at 299.880 kb on - strand, within SMa0564 at 299.921 kb on + strand, within SMa0564 at 299.921 kb on + strand, within SMa0564 at 299.921 kb on + strand, within SMa0564 at 299.922 kb on - strand, within SMa0564 at 299.922 kb on - strand, within SMa0564 at 300.050 kb on + strand, within SMa0564 at 300.051 kb on - strand, within SMa0564 at 300.211 kb on + strand, within SMa0564 at 300.265 kb on - strand, within SMa0564
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 297,336 + SMa0559 0.39 +0.9 297,337 - SMa0559 0.39 -0.5 297,339 + SMa0559 0.39 -0.8 297,556 + SMa0559 0.68 -0.2 297,556 + SMa0559 0.68 -2.6 297,557 - SMa0559 0.68 -2.1 297,557 - SMa0559 0.68 -0.4 297,557 - SMa0559 0.68 +1.4 297,852 + -2.1 297,852 + +0.9 297,853 - -0.6 297,853 - -2.0 297,951 + -1.0 297,951 + +0.9 297,951 + +0.4 297,952 - -0.3 297,952 - +0.2 297,952 - -0.1 298,031 + SMa0561 0.25 +0.9 298,031 + SMa0561 0.25 -1.1 298,032 - SMa0561 0.25 -1.8 298,080 - SMa0561 0.38 -1.6 298,080 - SMa0561 0.38 +0.2 298,080 - SMa0561 0.38 +1.2 298,116 - SMa0561 0.47 -1.7 298,239 - SMa0561 0.79 +0.1 298,296 + +0.4 298,392 + +0.2 298,491 + SMa0563 0.16 +0.9 298,491 + SMa0563 0.16 -1.2 298,491 + SMa0563 0.16 +0.2 298,492 - SMa0563 0.16 -2.1 298,492 - SMa0563 0.16 -0.9 298,492 - SMa0563 0.16 -0.1 298,682 + SMa0563 0.36 +0.5 298,861 - SMa0563 0.54 -1.3 299,093 + SMa0563 0.77 -0.1 299,206 + SMa0563 0.89 +0.2 299,206 + SMa0563 0.89 +0.4 299,379 + +3.4 299,472 + -0.5 299,473 - +2.3 299,568 - SMa0564 0.17 -0.1 299,765 + +0.9 299,766 - SMa0564 0.35 +0.1 299,772 - SMa0564 0.35 +0.9 299,772 - SMa0564 0.35 +0.9 299,877 + SMa0564 0.45 +1.0 299,878 - SMa0564 0.45 -0.6 299,879 + SMa0564 0.45 +0.2 299,879 + SMa0564 0.45 +4.3 299,880 - SMa0564 0.45 -3.0 299,880 - SMa0564 0.45 +0.6 299,921 + SMa0564 0.49 +0.4 299,921 + SMa0564 0.49 +1.3 299,921 + SMa0564 0.49 +0.9 299,922 - SMa0564 0.49 +0.6 299,922 - SMa0564 0.49 +0.1 300,050 + SMa0564 0.60 +1.6 300,051 - SMa0564 0.60 -0.5 300,211 + SMa0564 0.75 +1.3 300,265 - SMa0564 0.80 -1.1
Or see this region's nucleotide sequence