Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0501 and SMa0503 are separated by 1 nucleotides SMa0503 and SMa0506 are separated by 57 nucleotides SMa0506 and SMa0508 are separated by 49 nucleotides SMa0508 and SMa0510 are separated by 72 nucleotides
SMa0501: SMa0501 - ABC transporter permease, at 272,734 to 273,420
SMa0501
SMa0503: SMa0503 - ABC transporter permease, at 273,422 to 274,078
SMa0503
SMa0506: SMa0506 - ABC transporter periplasmic substrate-binding protein, at 274,136 to 274,933
SMa0506
SMa0508: SMa0508 - ABC transporter ATP-binding protein, at 274,983 to 275,714
SMa0508
SMa0510: SMa0510 - D-isomer specific 2-hydroxyacid dehydrogenase, at 275,787 to 276,734
SMa0510
Position (kb) 274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 273.142 kb on + strand, within SMa0501 at 273.143 kb on - strand, within SMa0501 at 273.325 kb on + strand, within SMa0501 at 273.325 kb on + strand, within SMa0501 at 273.325 kb on + strand, within SMa0501 at 273.326 kb on - strand, within SMa0501 at 273.326 kb on - strand, within SMa0501 at 273.462 kb on - strand at 273.462 kb on - strand at 273.462 kb on - strand at 273.584 kb on + strand, within SMa0503 at 273.584 kb on + strand, within SMa0503 at 273.584 kb on + strand, within SMa0503 at 273.585 kb on - strand, within SMa0503 at 273.585 kb on - strand, within SMa0503 at 273.585 kb on - strand, within SMa0503 at 273.635 kb on + strand, within SMa0503 at 273.635 kb on + strand, within SMa0503 at 273.638 kb on + strand, within SMa0503 at 273.639 kb on - strand, within SMa0503 at 273.800 kb on + strand, within SMa0503 at 273.905 kb on - strand, within SMa0503 at 273.998 kb on + strand, within SMa0503 at 273.998 kb on + strand, within SMa0503 at 274.234 kb on + strand, within SMa0506 at 274.454 kb on + strand, within SMa0506 at 274.493 kb on + strand, within SMa0506 at 274.494 kb on - strand, within SMa0506 at 274.716 kb on - strand, within SMa0506 at 274.716 kb on - strand, within SMa0506 at 274.716 kb on - strand, within SMa0506 at 274.776 kb on - strand, within SMa0506 at 274.789 kb on - strand, within SMa0506 at 274.790 kb on + strand, within SMa0506 at 274.790 kb on + strand, within SMa0506 at 275.047 kb on + strand at 275.244 kb on + strand, within SMa0508 at 275.245 kb on - strand, within SMa0508 at 275.245 kb on - strand, within SMa0508 at 275.386 kb on - strand, within SMa0508 at 275.708 kb on - strand at 275.803 kb on + strand at 275.803 kb on + strand at 275.803 kb on + strand at 275.804 kb on - strand at 275.804 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 273,142 + SMa0501 0.59 -0.4 273,143 - SMa0501 0.60 +0.9 273,325 + SMa0501 0.86 +0.1 273,325 + SMa0501 0.86 -0.6 273,325 + SMa0501 0.86 -1.1 273,326 - SMa0501 0.86 -1.5 273,326 - SMa0501 0.86 +0.9 273,462 - -1.1 273,462 - -1.0 273,462 - -0.2 273,584 + SMa0503 0.25 +3.9 273,584 + SMa0503 0.25 +4.3 273,584 + SMa0503 0.25 -0.5 273,585 - SMa0503 0.25 -2.1 273,585 - SMa0503 0.25 -0.4 273,585 - SMa0503 0.25 -1.7 273,635 + SMa0503 0.32 -3.3 273,635 + SMa0503 0.32 -0.8 273,638 + SMa0503 0.33 -0.6 273,639 - SMa0503 0.33 -0.7 273,800 + SMa0503 0.58 -0.7 273,905 - SMa0503 0.74 -2.3 273,998 + SMa0503 0.88 -1.7 273,998 + SMa0503 0.88 +3.3 274,234 + SMa0506 0.12 +2.0 274,454 + SMa0506 0.40 +1.1 274,493 + SMa0506 0.45 +0.8 274,494 - SMa0506 0.45 +0.7 274,716 - SMa0506 0.73 -0.1 274,716 - SMa0506 0.73 +0.4 274,716 - SMa0506 0.73 -1.2 274,776 - SMa0506 0.80 -0.9 274,789 - SMa0506 0.82 +1.1 274,790 + SMa0506 0.82 +2.1 274,790 + SMa0506 0.82 +0.3 275,047 + -0.4 275,244 + SMa0508 0.36 +0.7 275,245 - SMa0508 0.36 +4.3 275,245 - SMa0508 0.36 +0.9 275,386 - SMa0508 0.55 +0.6 275,708 - -0.4 275,803 + -0.7 275,803 + +0.1 275,803 + +0.1 275,804 - +0.4 275,804 - +0.6
Or see this region's nucleotide sequence