Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0341 and SMa0343 are separated by 184 nucleotides SMa0343 and SMa0346 are separated by 3 nucleotides SMa0346 and SMa0347 are separated by 47 nucleotides
SMa0341: SMa0341 - hypothetical protein, at 186,524 to 186,718
SMa0341
SMa0343: SMa0343 - hypothetical protein, at 186,903 to 187,337
SMa0343
SMa0346: SMa0346 - NAD(P)H dehydrogenase, at 187,341 to 187,958
SMa0346
SMa0347: SMa0347 - LysR family transcriptional regulator, at 188,006 to 188,893
SMa0347
Position (kb) 187
188 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 186.479 kb on + strand at 186.480 kb on - strand at 186.529 kb on + strand at 186.564 kb on - strand, within SMa0341 at 186.622 kb on + strand, within SMa0341 at 186.751 kb on + strand at 186.829 kb on + strand at 186.829 kb on + strand at 186.904 kb on + strand at 187.072 kb on + strand at 187.198 kb on - strand, within SMa0343 at 187.198 kb on - strand, within SMa0343 at 187.362 kb on + strand at 187.453 kb on + strand, within SMa0346 at 187.454 kb on - strand, within SMa0346 at 187.518 kb on + strand, within SMa0346 at 187.634 kb on - strand, within SMa0346 at 187.642 kb on + strand, within SMa0346 at 187.642 kb on + strand, within SMa0346 at 187.643 kb on - strand, within SMa0346 at 187.699 kb on + strand, within SMa0346 at 187.728 kb on - strand, within SMa0346 at 187.778 kb on + strand, within SMa0346 at 187.778 kb on + strand, within SMa0346 at 187.778 kb on + strand, within SMa0346 at 187.779 kb on - strand, within SMa0346 at 187.779 kb on - strand, within SMa0346 at 187.779 kb on - strand, within SMa0346 at 187.967 kb on + strand at 187.986 kb on + strand at 187.986 kb on + strand at 187.987 kb on - strand at 187.987 kb on - strand at 187.987 kb on - strand at 187.987 kb on - strand at 187.987 kb on - strand at 187.993 kb on + strand at 187.993 kb on + strand at 187.997 kb on - strand at 188.028 kb on + strand at 188.179 kb on + strand, within SMa0347 at 188.224 kb on + strand, within SMa0347 at 188.224 kb on + strand, within SMa0347 at 188.224 kb on + strand, within SMa0347 at 188.225 kb on - strand, within SMa0347 at 188.225 kb on - strand, within SMa0347 at 188.242 kb on + strand, within SMa0347 at 188.242 kb on + strand, within SMa0347 at 188.243 kb on - strand, within SMa0347 at 188.272 kb on - strand, within SMa0347 at 188.358 kb on + strand at 188.359 kb on - strand, within SMa0347 at 188.516 kb on + strand, within SMa0347 at 188.516 kb on + strand, within SMa0347 at 188.516 kb on - strand, within SMa0347 at 188.517 kb on - strand, within SMa0347 at 188.566 kb on + strand, within SMa0347 at 188.871 kb on + strand at 188.871 kb on + strand at 188.872 kb on - strand at 188.902 kb on + strand at 188.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 186,479 + +1.8 186,480 - +1.3 186,529 + -2.9 186,564 - SMa0341 0.21 -1.4 186,622 + SMa0341 0.50 +0.7 186,751 + -1.1 186,829 + +1.0 186,829 + -0.2 186,904 + +0.3 187,072 + -1.7 187,198 - SMa0343 0.68 -0.0 187,198 - SMa0343 0.68 -0.5 187,362 + +0.4 187,453 + SMa0346 0.18 -1.5 187,454 - SMa0346 0.18 -3.1 187,518 + SMa0346 0.29 -0.5 187,634 - SMa0346 0.47 -0.1 187,642 + SMa0346 0.49 -1.5 187,642 + SMa0346 0.49 +0.1 187,643 - SMa0346 0.49 +0.3 187,699 + SMa0346 0.58 -2.6 187,728 - SMa0346 0.63 +1.0 187,778 + SMa0346 0.71 -0.0 187,778 + SMa0346 0.71 -0.9 187,778 + SMa0346 0.71 -3.6 187,779 - SMa0346 0.71 -2.7 187,779 - SMa0346 0.71 +1.6 187,779 - SMa0346 0.71 -0.7 187,967 + +0.7 187,986 + -0.2 187,986 + -2.0 187,987 - -2.4 187,987 - +0.7 187,987 - +1.3 187,987 - +0.9 187,987 - -0.7 187,993 + -0.0 187,993 + +0.3 187,997 - +1.2 188,028 + -1.1 188,179 + SMa0347 0.19 +1.3 188,224 + SMa0347 0.25 +1.5 188,224 + SMa0347 0.25 +0.5 188,224 + SMa0347 0.25 +0.9 188,225 - SMa0347 0.25 +0.4 188,225 - SMa0347 0.25 +0.4 188,242 + SMa0347 0.27 -0.3 188,242 + SMa0347 0.27 -2.4 188,243 - SMa0347 0.27 -1.9 188,272 - SMa0347 0.30 +0.6 188,358 + +0.9 188,359 - SMa0347 0.40 -0.3 188,516 + SMa0347 0.57 +0.4 188,516 + SMa0347 0.57 -3.0 188,516 - SMa0347 0.57 -3.8 188,517 - SMa0347 0.58 -0.1 188,566 + SMa0347 0.63 -0.2 188,871 + -0.6 188,871 + +0.9 188,872 - -0.3 188,902 + -3.1 188,903 - +0.9
Or see this region's nucleotide sequence