Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0298 and SMa0299 are separated by 2 nucleotides SMa0299 and SMa0300 overlap by 4 nucleotides SMa0300 and SMa0301 overlap by 4 nucleotides SMa0301 and SMa0302 are separated by 69 nucleotides
SMa0298: SMa0298 - ABC transporter ATP-binding protein, at 163,041 to 164,855
SMa0298
SMa0299: SMa0299 - ABC transporter permease, at 164,858 to 165,745
SMa0299
SMa0300: SMa0300 - ABC transporter permease, at 165,742 to 166,695
SMa0300
SMa0301: SMa0301 - hypothetical protein, at 166,692 to 167,360
SMa0301
SMa0302: SMa0302 - ABC transporter substrate-binding protein, at 167,430 to 168,956
SMa0302
Position (kb) 165
166
167 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 164.815 kb on + strand at 164.816 kb on - strand at 164.856 kb on - strand at 164.978 kb on + strand, within SMa0299 at 164.979 kb on - strand, within SMa0299 at 165.082 kb on - strand, within SMa0299 at 165.085 kb on + strand, within SMa0299 at 165.085 kb on + strand, within SMa0299 at 165.086 kb on - strand, within SMa0299 at 165.086 kb on - strand, within SMa0299 at 165.161 kb on - strand, within SMa0299 at 165.192 kb on + strand, within SMa0299 at 165.193 kb on - strand, within SMa0299 at 165.252 kb on + strand, within SMa0299 at 165.403 kb on + strand, within SMa0299 at 165.403 kb on + strand, within SMa0299 at 165.406 kb on - strand, within SMa0299 at 165.481 kb on + strand, within SMa0299 at 165.482 kb on - strand, within SMa0299 at 165.598 kb on - strand, within SMa0299 at 165.736 kb on + strand at 165.813 kb on - strand at 165.813 kb on - strand at 165.902 kb on + strand, within SMa0300 at 165.903 kb on - strand, within SMa0300 at 166.055 kb on + strand, within SMa0300 at 166.055 kb on + strand, within SMa0300 at 166.055 kb on + strand, within SMa0300 at 166.055 kb on + strand, within SMa0300 at 166.056 kb on - strand, within SMa0300 at 166.107 kb on - strand, within SMa0300 at 166.119 kb on + strand, within SMa0300 at 166.120 kb on - strand, within SMa0300 at 166.120 kb on - strand, within SMa0300 at 166.364 kb on + strand, within SMa0300 at 166.365 kb on - strand, within SMa0300 at 166.496 kb on + strand, within SMa0300 at 166.685 kb on - strand at 166.685 kb on - strand at 166.685 kb on - strand at 166.685 kb on - strand at 166.789 kb on + strand, within SMa0301 at 166.789 kb on + strand, within SMa0301 at 166.789 kb on + strand, within SMa0301 at 166.790 kb on - strand, within SMa0301 at 166.790 kb on - strand, within SMa0301 at 166.790 kb on - strand, within SMa0301 at 166.925 kb on - strand, within SMa0301 at 166.927 kb on + strand, within SMa0301 at 167.000 kb on + strand, within SMa0301 at 167.001 kb on - strand, within SMa0301 at 167.034 kb on - strand, within SMa0301 at 167.056 kb on + strand, within SMa0301 at 167.057 kb on - strand, within SMa0301 at 167.101 kb on + strand, within SMa0301 at 167.101 kb on + strand, within SMa0301 at 167.102 kb on - strand, within SMa0301 at 167.366 kb on + strand at 167.366 kb on + strand at 167.367 kb on - strand at 167.456 kb on - strand at 167.456 kb on - strand at 167.488 kb on + strand at 167.546 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 164,815 + +0.1 164,816 - -0.1 164,856 - +1.7 164,978 + SMa0299 0.14 -1.3 164,979 - SMa0299 0.14 -1.8 165,082 - SMa0299 0.25 -0.4 165,085 + SMa0299 0.26 +0.8 165,085 + SMa0299 0.26 +0.9 165,086 - SMa0299 0.26 -1.5 165,086 - SMa0299 0.26 -0.3 165,161 - SMa0299 0.34 -0.4 165,192 + SMa0299 0.38 -0.4 165,193 - SMa0299 0.38 +1.3 165,252 + SMa0299 0.44 -0.1 165,403 + SMa0299 0.61 +2.4 165,403 + SMa0299 0.61 -1.1 165,406 - SMa0299 0.62 +0.1 165,481 + SMa0299 0.70 -2.4 165,482 - SMa0299 0.70 -1.4 165,598 - SMa0299 0.83 +0.6 165,736 + +1.5 165,813 - -1.2 165,813 - +1.3 165,902 + SMa0300 0.17 +0.8 165,903 - SMa0300 0.17 -1.3 166,055 + SMa0300 0.33 +0.5 166,055 + SMa0300 0.33 +0.9 166,055 + SMa0300 0.33 +0.9 166,055 + SMa0300 0.33 +0.7 166,056 - SMa0300 0.33 +0.9 166,107 - SMa0300 0.38 -0.9 166,119 + SMa0300 0.40 +0.6 166,120 - SMa0300 0.40 +1.5 166,120 - SMa0300 0.40 -0.4 166,364 + SMa0300 0.65 -0.1 166,365 - SMa0300 0.65 -3.0 166,496 + SMa0300 0.79 -0.1 166,685 - -2.3 166,685 - +1.8 166,685 - -2.9 166,685 - +1.8 166,789 + SMa0301 0.14 +0.7 166,789 + SMa0301 0.14 -0.1 166,789 + SMa0301 0.14 -2.5 166,790 - SMa0301 0.15 +0.8 166,790 - SMa0301 0.15 -1.6 166,790 - SMa0301 0.15 -0.8 166,925 - SMa0301 0.35 -1.1 166,927 + SMa0301 0.35 -1.7 167,000 + SMa0301 0.46 +1.1 167,001 - SMa0301 0.46 -1.4 167,034 - SMa0301 0.51 +0.9 167,056 + SMa0301 0.54 +0.2 167,057 - SMa0301 0.55 -1.9 167,101 + SMa0301 0.61 +0.7 167,101 + SMa0301 0.61 -2.1 167,102 - SMa0301 0.61 +0.3 167,366 + +5.0 167,366 + +1.0 167,367 - -1.0 167,456 - -0.7 167,456 - -0.8 167,488 + +1.1 167,546 + +1.7
Or see this region's nucleotide sequence