Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0293 and SMa0298 are separated by 372 nucleotides SMa0298 and SMa0299 are separated by 2 nucleotides SMa0299 and SMa0300 overlap by 4 nucleotides
SMa0293: SMa0293 - hypothetical protein, at 162,273 to 162,668
SMa0293
SMa0298: SMa0298 - ABC transporter ATP-binding protein, at 163,041 to 164,855
SMa0298
SMa0299: SMa0299 - ABC transporter permease, at 164,858 to 165,745
SMa0299
SMa0300: SMa0300 - ABC transporter permease, at 165,742 to 166,695
SMa0300
Position (kb) 163
164
165 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 162.085 kb on + strand at 162.262 kb on - strand at 162.274 kb on + strand at 162.369 kb on - strand, within SMa0293 at 162.374 kb on + strand, within SMa0293 at 162.421 kb on + strand, within SMa0293 at 162.514 kb on - strand, within SMa0293 at 162.599 kb on + strand, within SMa0293 at 162.651 kb on + strand at 162.651 kb on + strand at 162.686 kb on + strand at 162.687 kb on - strand at 162.687 kb on - strand at 162.820 kb on - strand at 162.839 kb on - strand at 162.976 kb on + strand at 162.983 kb on + strand at 163.039 kb on + strand at 163.039 kb on + strand at 163.039 kb on + strand at 163.040 kb on - strand at 163.040 kb on - strand at 163.184 kb on - strand at 163.204 kb on - strand at 163.263 kb on + strand, within SMa0298 at 163.263 kb on + strand, within SMa0298 at 163.264 kb on - strand at 163.264 kb on - strand, within SMa0298 at 163.264 kb on - strand, within SMa0298 at 163.355 kb on + strand, within SMa0298 at 163.356 kb on - strand, within SMa0298 at 163.356 kb on - strand, within SMa0298 at 163.552 kb on + strand, within SMa0298 at 163.553 kb on - strand, within SMa0298 at 163.778 kb on + strand, within SMa0298 at 163.779 kb on - strand, within SMa0298 at 163.812 kb on + strand, within SMa0298 at 163.812 kb on + strand, within SMa0298 at 163.813 kb on - strand, within SMa0298 at 163.813 kb on - strand, within SMa0298 at 163.813 kb on - strand, within SMa0298 at 163.845 kb on - strand, within SMa0298 at 164.113 kb on + strand, within SMa0298 at 164.113 kb on + strand, within SMa0298 at 164.141 kb on + strand, within SMa0298 at 164.176 kb on + strand, within SMa0298 at 164.177 kb on - strand, within SMa0298 at 164.177 kb on - strand, within SMa0298 at 164.177 kb on - strand, within SMa0298 at 164.177 kb on - strand, within SMa0298 at 164.177 kb on - strand, within SMa0298 at 164.297 kb on + strand, within SMa0298 at 164.298 kb on - strand, within SMa0298 at 164.457 kb on + strand, within SMa0298 at 164.815 kb on + strand at 164.816 kb on - strand at 164.856 kb on - strand at 164.978 kb on + strand, within SMa0299 at 164.979 kb on - strand, within SMa0299 at 165.082 kb on - strand, within SMa0299 at 165.085 kb on + strand, within SMa0299 at 165.085 kb on + strand, within SMa0299 at 165.086 kb on - strand, within SMa0299 at 165.086 kb on - strand, within SMa0299 at 165.161 kb on - strand, within SMa0299 at 165.192 kb on + strand, within SMa0299 at 165.193 kb on - strand, within SMa0299 at 165.252 kb on + strand, within SMa0299 at 165.403 kb on + strand, within SMa0299 at 165.403 kb on + strand, within SMa0299 at 165.406 kb on - strand, within SMa0299 at 165.481 kb on + strand, within SMa0299 at 165.482 kb on - strand, within SMa0299 at 165.598 kb on - strand, within SMa0299 at 165.736 kb on + strand at 165.813 kb on - strand at 165.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 162,085 + -0.1 162,262 - -2.5 162,274 + -1.5 162,369 - SMa0293 0.24 -3.3 162,374 + SMa0293 0.26 +0.2 162,421 + SMa0293 0.37 -1.5 162,514 - SMa0293 0.61 +1.2 162,599 + SMa0293 0.82 +0.2 162,651 + -2.8 162,651 + -2.3 162,686 + -0.1 162,687 - -1.3 162,687 - +0.4 162,820 - -2.7 162,839 - +0.6 162,976 + +0.5 162,983 + +0.2 163,039 + +1.1 163,039 + +1.5 163,039 + -1.8 163,040 - +0.8 163,040 - -0.3 163,184 - +0.4 163,204 - +1.9 163,263 + SMa0298 0.12 +0.6 163,263 + SMa0298 0.12 -0.5 163,264 - +0.9 163,264 - SMa0298 0.12 -1.1 163,264 - SMa0298 0.12 +3.2 163,355 + SMa0298 0.17 +0.9 163,356 - SMa0298 0.17 +0.1 163,356 - SMa0298 0.17 -0.8 163,552 + SMa0298 0.28 +0.6 163,553 - SMa0298 0.28 -1.2 163,778 + SMa0298 0.41 +0.4 163,779 - SMa0298 0.41 -2.1 163,812 + SMa0298 0.42 -0.0 163,812 + SMa0298 0.42 +1.3 163,813 - SMa0298 0.43 +0.1 163,813 - SMa0298 0.43 -1.5 163,813 - SMa0298 0.43 -1.1 163,845 - SMa0298 0.44 -1.4 164,113 + SMa0298 0.59 -0.6 164,113 + SMa0298 0.59 -0.6 164,141 + SMa0298 0.61 -1.9 164,176 + SMa0298 0.63 -0.5 164,177 - SMa0298 0.63 -3.1 164,177 - SMa0298 0.63 -0.5 164,177 - SMa0298 0.63 -2.9 164,177 - SMa0298 0.63 +0.1 164,177 - SMa0298 0.63 +0.5 164,297 + SMa0298 0.69 +0.4 164,298 - SMa0298 0.69 -0.4 164,457 + SMa0298 0.78 -1.7 164,815 + +0.1 164,816 - -0.1 164,856 - +1.7 164,978 + SMa0299 0.14 -1.3 164,979 - SMa0299 0.14 -1.8 165,082 - SMa0299 0.25 -0.4 165,085 + SMa0299 0.26 +0.8 165,085 + SMa0299 0.26 +0.9 165,086 - SMa0299 0.26 -1.5 165,086 - SMa0299 0.26 -0.3 165,161 - SMa0299 0.34 -0.4 165,192 + SMa0299 0.38 -0.4 165,193 - SMa0299 0.38 +1.3 165,252 + SMa0299 0.44 -0.1 165,403 + SMa0299 0.61 +2.4 165,403 + SMa0299 0.61 -1.1 165,406 - SMa0299 0.62 +0.1 165,481 + SMa0299 0.70 -2.4 165,482 - SMa0299 0.70 -1.4 165,598 - SMa0299 0.83 +0.6 165,736 + +1.5 165,813 - -1.2 165,813 - +1.3
Or see this region's nucleotide sequence