Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0235 and SMa0237 are separated by 8 nucleotides SMa0237 and SMa0241 overlap by 4 nucleotides SMa0241 and SMa0244 are separated by 194 nucleotides
SMa0235: SMa0235 - dihydroxy-acid dehydratase, at 131,106 to 132,884
SMa0235
SMa0237: SMa0237 - dehydrogenase, at 132,893 to 133,774
SMa0237
SMa0241: SMa0241 - epimerase, at 133,771 to 134,754
SMa0241
SMa0244: SMa0244 - Dehydrogenase, FAD-dependent, at 134,949 to 136,394
SMa0244
Position (kb) 133
134
135 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 132.805 kb on + strand at 132.806 kb on - strand at 132.825 kb on - strand at 132.825 kb on - strand at 133.375 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.504 kb on + strand, within SMa0237 at 133.505 kb on - strand, within SMa0237 at 133.505 kb on - strand, within SMa0237 at 133.519 kb on + strand, within SMa0237 at 133.589 kb on - strand, within SMa0237 at 133.685 kb on - strand, within SMa0237 at 134.261 kb on + strand, within SMa0241 at 134.262 kb on - strand, within SMa0241 at 134.303 kb on + strand, within SMa0241 at 134.304 kb on - strand, within SMa0241 at 134.319 kb on + strand, within SMa0241 at 134.320 kb on - strand, within SMa0241 at 134.320 kb on - strand, within SMa0241 at 134.327 kb on + strand, within SMa0241 at 134.328 kb on - strand, within SMa0241 at 134.402 kb on + strand, within SMa0241 at 134.402 kb on + strand, within SMa0241 at 134.638 kb on + strand, within SMa0241 at 134.639 kb on - strand, within SMa0241 at 134.639 kb on - strand, within SMa0241 at 134.749 kb on - strand at 134.840 kb on + strand at 134.864 kb on + strand at 134.865 kb on - strand at 134.968 kb on + strand at 135.030 kb on - strand at 135.643 kb on + strand, within SMa0244
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 132,805 + -0.6 132,806 - -1.0 132,825 - -1.2 132,825 - -0.9 133,375 + SMa0237 0.55 -1.4 133,504 + SMa0237 0.69 +1.3 133,504 + SMa0237 0.69 +0.5 133,504 + SMa0237 0.69 -0.3 133,504 + SMa0237 0.69 -1.5 133,505 - SMa0237 0.69 -0.9 133,505 - SMa0237 0.69 -3.5 133,519 + SMa0237 0.71 -2.7 133,589 - SMa0237 0.79 +1.4 133,685 - SMa0237 0.90 +0.8 134,261 + SMa0241 0.50 -1.8 134,262 - SMa0241 0.50 -1.2 134,303 + SMa0241 0.54 -1.1 134,304 - SMa0241 0.54 +0.9 134,319 + SMa0241 0.56 -0.4 134,320 - SMa0241 0.56 -0.9 134,320 - SMa0241 0.56 -1.2 134,327 + SMa0241 0.57 -1.4 134,328 - SMa0241 0.57 -0.3 134,402 + SMa0241 0.64 +0.0 134,402 + SMa0241 0.64 -1.5 134,638 + SMa0241 0.88 +0.2 134,639 - SMa0241 0.88 +0.6 134,639 - SMa0241 0.88 +1.0 134,749 - -1.2 134,840 + +0.9 134,864 + -1.1 134,865 - -0.4 134,968 + +0.6 135,030 - +1.2 135,643 + SMa0244 0.48 +2.5
Or see this region's nucleotide sequence