Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0106 and SMa0108 overlap by 4 nucleotides SMa0108 and SMa0110 overlap by 4 nucleotides SMa0110 and SMa5001 are separated by 41 nucleotides SMa5001 and SMa0112 are separated by 190 nucleotides
SMa0106: SMa0106 - ABC transporter permease, at 55,565 to 56,386
SMa0106
SMa0108: SMa0108 - ABC transporter ATP-binding protein, at 56,383 to 57,297
SMa0108
SMa0110: SMa0110 - ABC transporter ATP-binding protein, at 57,294 to 58,256
SMa0110
SMa5001: SMa5001 - hypothetical protein, at 58,298 to 58,612
SMa5001
SMa0112: SMa0112 - hypothetical protein, at 58,803 to 59,234
SMa0112
Position (kb) 57
58
59 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 56.497 kb on + strand, within SMa0108 at 56.497 kb on + strand, within SMa0108 at 56.580 kb on - strand, within SMa0108 at 56.840 kb on - strand, within SMa0108 at 56.876 kb on + strand, within SMa0108 at 56.969 kb on + strand, within SMa0108 at 56.970 kb on - strand, within SMa0108 at 57.073 kb on + strand, within SMa0108 at 57.073 kb on + strand, within SMa0108 at 57.136 kb on + strand, within SMa0108 at 57.136 kb on + strand, within SMa0108 at 57.136 kb on + strand, within SMa0108 at 57.137 kb on - strand, within SMa0108 at 57.137 kb on - strand, within SMa0108 at 57.139 kb on - strand, within SMa0108 at 57.603 kb on + strand, within SMa0110 at 57.603 kb on + strand, within SMa0110 at 57.959 kb on + strand, within SMa0110 at 57.959 kb on + strand, within SMa0110 at 57.959 kb on + strand, within SMa0110 at 57.959 kb on + strand, within SMa0110 at 57.960 kb on - strand, within SMa0110 at 57.960 kb on - strand, within SMa0110 at 58.050 kb on - strand, within SMa0110 at 58.233 kb on - strand at 58.440 kb on + strand, within SMa5001 at 58.472 kb on + strand, within SMa5001 at 58.473 kb on - strand, within SMa5001 at 58.534 kb on + strand, within SMa5001 at 58.534 kb on + strand, within SMa5001 at 58.534 kb on + strand, within SMa5001 at 58.535 kb on - strand, within SMa5001 at 58.536 kb on + strand, within SMa5001 at 58.537 kb on - strand, within SMa5001 at 58.537 kb on - strand, within SMa5001 at 58.537 kb on - strand, within SMa5001 at 58.537 kb on - strand, within SMa5001 at 58.566 kb on + strand, within SMa5001 at 58.567 kb on - strand, within SMa5001 at 58.602 kb on + strand at 58.624 kb on + strand at 58.796 kb on - strand at 58.839 kb on + strand at 58.929 kb on + strand, within SMa0112 at 58.936 kb on - strand, within SMa0112 at 59.033 kb on + strand, within SMa0112 at 59.049 kb on - strand, within SMa0112 at 59.083 kb on + strand, within SMa0112 at 59.168 kb on + strand, within SMa0112 at 59.217 kb on + strand at 59.249 kb on + strand at 59.249 kb on + strand at 59.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 56,497 + SMa0108 0.12 +0.1 56,497 + SMa0108 0.12 +1.4 56,580 - SMa0108 0.22 +0.3 56,840 - SMa0108 0.50 +0.4 56,876 + SMa0108 0.54 -1.7 56,969 + SMa0108 0.64 +0.5 56,970 - SMa0108 0.64 -0.0 57,073 + SMa0108 0.75 -0.0 57,073 + SMa0108 0.75 -1.8 57,136 + SMa0108 0.82 -0.7 57,136 + SMa0108 0.82 -0.6 57,136 + SMa0108 0.82 +0.9 57,137 - SMa0108 0.82 -1.0 57,137 - SMa0108 0.82 -1.0 57,139 - SMa0108 0.83 -0.2 57,603 + SMa0110 0.32 +0.9 57,603 + SMa0110 0.32 -1.5 57,959 + SMa0110 0.69 +0.7 57,959 + SMa0110 0.69 -1.4 57,959 + SMa0110 0.69 -0.3 57,959 + SMa0110 0.69 -0.2 57,960 - SMa0110 0.69 -1.9 57,960 - SMa0110 0.69 -0.1 58,050 - SMa0110 0.79 +1.9 58,233 - -1.7 58,440 + SMa5001 0.45 -1.3 58,472 + SMa5001 0.55 -2.2 58,473 - SMa5001 0.56 -0.3 58,534 + SMa5001 0.75 -0.2 58,534 + SMa5001 0.75 -3.8 58,534 + SMa5001 0.75 -2.3 58,535 - SMa5001 0.75 -2.5 58,536 + SMa5001 0.76 -0.1 58,537 - SMa5001 0.76 -0.9 58,537 - SMa5001 0.76 +2.6 58,537 - SMa5001 0.76 +0.9 58,537 - SMa5001 0.76 -1.2 58,566 + SMa5001 0.85 -2.3 58,567 - SMa5001 0.85 +1.1 58,602 + +0.9 58,624 + -1.5 58,796 - +0.2 58,839 + +2.2 58,929 + SMa0112 0.29 -1.7 58,936 - SMa0112 0.31 -1.7 59,033 + SMa0112 0.53 -0.3 59,049 - SMa0112 0.57 +1.3 59,083 + SMa0112 0.65 -0.8 59,168 + SMa0112 0.84 -1.7 59,217 + -1.1 59,249 + -2.3 59,249 + -0.7 59,250 - -0.4
Or see this region's nucleotide sequence