Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21505 and SM_b21506 are separated by 12 nucleotides SM_b21506 and SM_b21507 are separated by 202 nucleotides SM_b21507 and SM_b21508 are separated by 40 nucleotides
SM_b21505: SM_b21505 - membrane protein, Wzy-like polysaccharide polymerase ExoQ, at 1,429,976 to 1,431,307
_b21505
SM_b21506: SM_b21506 - cell surface polysaccharides export protein, at 1,431,320 to 1,432,936
_b21506
SM_b21507: SM_b21507 - amino acid transporter, exporter protein, at 1,433,139 to 1,433,795
_b21507
SM_b21508: SM_b21508 - dihydrodipicolinate synthetase, at 1,433,836 to 1,434,750
_b21508
Position (kb)
1431
1432
1433 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1430.341 kb on - strand, within SM_b21505 at 1430.380 kb on - strand, within SM_b21505 at 1430.380 kb on - strand, within SM_b21505 at 1430.706 kb on + strand, within SM_b21505 at 1430.707 kb on - strand, within SM_b21505 at 1430.707 kb on - strand, within SM_b21505 at 1430.850 kb on + strand, within SM_b21505 at 1430.851 kb on - strand, within SM_b21505 at 1430.853 kb on + strand, within SM_b21505 at 1430.854 kb on - strand, within SM_b21505 at 1430.931 kb on + strand, within SM_b21505 at 1430.932 kb on - strand, within SM_b21505 at 1430.932 kb on - strand, within SM_b21505 at 1431.434 kb on - strand at 1431.550 kb on - strand, within SM_b21506 at 1431.793 kb on - strand, within SM_b21506 at 1431.793 kb on - strand, within SM_b21506 at 1431.796 kb on + strand, within SM_b21506 at 1431.796 kb on + strand, within SM_b21506 at 1431.832 kb on + strand, within SM_b21506 at 1431.834 kb on + strand, within SM_b21506 at 1431.834 kb on + strand, within SM_b21506 at 1431.835 kb on - strand, within SM_b21506 at 1431.835 kb on - strand, within SM_b21506 at 1432.295 kb on + strand, within SM_b21506 at 1432.295 kb on + strand, within SM_b21506 at 1432.489 kb on + strand, within SM_b21506 at 1432.490 kb on - strand, within SM_b21506 at 1432.546 kb on - strand, within SM_b21506 at 1432.546 kb on - strand, within SM_b21506 at 1432.578 kb on + strand, within SM_b21506 at 1432.579 kb on - strand, within SM_b21506 at 1432.579 kb on - strand, within SM_b21506 at 1432.762 kb on - strand, within SM_b21506 at 1432.766 kb on + strand, within SM_b21506 at 1432.901 kb on + strand at 1432.901 kb on + strand at 1432.902 kb on - strand at 1432.932 kb on + strand at 1432.933 kb on - strand at 1432.973 kb on + strand at 1432.974 kb on - strand at 1433.006 kb on - strand at 1433.006 kb on - strand at 1433.091 kb on + strand at 1433.092 kb on - strand at 1433.157 kb on + strand at 1433.158 kb on - strand at 1433.158 kb on - strand at 1433.158 kb on - strand at 1433.158 kb on - strand at 1433.158 kb on - strand at 1433.188 kb on - strand at 1433.273 kb on + strand, within SM_b21507 at 1433.306 kb on - strand, within SM_b21507 at 1433.359 kb on - strand, within SM_b21507 at 1433.359 kb on - strand, within SM_b21507 at 1433.397 kb on + strand, within SM_b21507 at 1433.398 kb on - strand, within SM_b21507 at 1433.429 kb on + strand, within SM_b21507 at 1433.624 kb on + strand, within SM_b21507 at 1433.752 kb on + strand at 1433.753 kb on - strand at 1433.753 kb on - strand at 1433.753 kb on - strand at 1433.822 kb on + strand at 1433.822 kb on + strand at 1433.830 kb on - strand at 1433.830 kb on - strand at 1433.887 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,430,341 - SM_b21505 0.27 -1.1 1,430,380 - SM_b21505 0.30 +1.0 1,430,380 - SM_b21505 0.30 -2.8 1,430,706 + SM_b21505 0.55 +0.1 1,430,707 - SM_b21505 0.55 -0.4 1,430,707 - SM_b21505 0.55 -0.2 1,430,850 + SM_b21505 0.66 +0.9 1,430,851 - SM_b21505 0.66 +0.3 1,430,853 + SM_b21505 0.66 -0.6 1,430,854 - SM_b21505 0.66 -0.6 1,430,931 + SM_b21505 0.72 +1.7 1,430,932 - SM_b21505 0.72 +1.1 1,430,932 - SM_b21505 0.72 +1.1 1,431,434 - -1.3 1,431,550 - SM_b21506 0.14 -0.8 1,431,793 - SM_b21506 0.29 +0.5 1,431,793 - SM_b21506 0.29 -1.6 1,431,796 + SM_b21506 0.29 -0.4 1,431,796 + SM_b21506 0.29 -1.8 1,431,832 + SM_b21506 0.32 +3.3 1,431,834 + SM_b21506 0.32 -2.7 1,431,834 + SM_b21506 0.32 -2.9 1,431,835 - SM_b21506 0.32 +0.3 1,431,835 - SM_b21506 0.32 +3.5 1,432,295 + SM_b21506 0.60 +0.1 1,432,295 + SM_b21506 0.60 -1.0 1,432,489 + SM_b21506 0.72 -1.8 1,432,490 - SM_b21506 0.72 -0.1 1,432,546 - SM_b21506 0.76 -2.5 1,432,546 - SM_b21506 0.76 +0.5 1,432,578 + SM_b21506 0.78 +0.0 1,432,579 - SM_b21506 0.78 +0.1 1,432,579 - SM_b21506 0.78 -0.1 1,432,762 - SM_b21506 0.89 -3.5 1,432,766 + SM_b21506 0.89 -1.6 1,432,901 + -1.4 1,432,901 + -0.6 1,432,902 - -0.1 1,432,932 + -0.3 1,432,933 - -0.1 1,432,973 + -2.4 1,432,974 - -0.5 1,433,006 - +0.1 1,433,006 - +0.4 1,433,091 + +0.1 1,433,092 - +1.0 1,433,157 + +2.0 1,433,158 - -0.1 1,433,158 - -4.3 1,433,158 - -2.5 1,433,158 - -0.9 1,433,158 - +0.4 1,433,188 - -1.4 1,433,273 + SM_b21507 0.20 -3.2 1,433,306 - SM_b21507 0.25 +0.7 1,433,359 - SM_b21507 0.33 +0.2 1,433,359 - SM_b21507 0.33 +0.1 1,433,397 + SM_b21507 0.39 -3.3 1,433,398 - SM_b21507 0.39 +0.0 1,433,429 + SM_b21507 0.44 -0.3 1,433,624 + SM_b21507 0.74 +0.9 1,433,752 + +0.3 1,433,753 - -0.1 1,433,753 - -1.1 1,433,753 - +2.9 1,433,822 + -0.5 1,433,822 + -0.1 1,433,830 - +1.4 1,433,830 - -2.1 1,433,887 + -3.9
Or see this region's nucleotide sequence