Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21502 and SM_b21503 are separated by 231 nucleotides SM_b21503 and SM_b21504 are separated by 10 nucleotides SM_b21504 and SM_b21505 overlap by 8 nucleotides
SM_b21502: SM_b21502 - glycosyltransferase, at 1,426,473 to 1,427,642
_b21502
SM_b21503: SM_b21503 - glycosyltransferase, at 1,427,874 to 1,429,217
_b21503
SM_b21504: SM_b21504 - hypothetical protein, at 1,429,228 to 1,429,983
_b21504
SM_b21505: SM_b21505 - membrane protein, Wzy-like polysaccharide polymerase ExoQ, at 1,429,976 to 1,431,307
_b21505
Position (kb)
1427
1428
1429
1430 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1426.936 kb on + strand, within SM_b21502 at 1426.937 kb on - strand, within SM_b21502 at 1426.937 kb on - strand, within SM_b21502 at 1426.996 kb on + strand, within SM_b21502 at 1426.996 kb on + strand, within SM_b21502 at 1426.997 kb on - strand, within SM_b21502 at 1426.997 kb on - strand, within SM_b21502 at 1427.016 kb on + strand, within SM_b21502 at 1427.095 kb on + strand, within SM_b21502 at 1427.095 kb on + strand, within SM_b21502 at 1427.221 kb on + strand, within SM_b21502 at 1427.221 kb on + strand, within SM_b21502 at 1427.278 kb on - strand, within SM_b21502 at 1427.338 kb on + strand, within SM_b21502 at 1427.338 kb on + strand, within SM_b21502 at 1427.339 kb on - strand, within SM_b21502 at 1427.343 kb on + strand, within SM_b21502 at 1427.343 kb on + strand, within SM_b21502 at 1427.343 kb on + strand, within SM_b21502 at 1427.343 kb on + strand, within SM_b21502 at 1427.343 kb on + strand, within SM_b21502 at 1427.343 kb on + strand, within SM_b21502 at 1427.344 kb on - strand, within SM_b21502 at 1427.344 kb on - strand, within SM_b21502 at 1427.344 kb on - strand, within SM_b21502 at 1427.357 kb on + strand, within SM_b21502 at 1427.358 kb on - strand, within SM_b21502 at 1427.358 kb on - strand, within SM_b21502 at 1427.384 kb on + strand, within SM_b21502 at 1427.384 kb on + strand, within SM_b21502 at 1427.384 kb on + strand, within SM_b21502 at 1427.384 kb on + strand, within SM_b21502 at 1427.385 kb on - strand, within SM_b21502 at 1427.385 kb on - strand, within SM_b21502 at 1427.385 kb on - strand, within SM_b21502 at 1427.385 kb on - strand, within SM_b21502 at 1427.385 kb on - strand, within SM_b21502 at 1427.385 kb on - strand, within SM_b21502 at 1427.742 kb on - strand at 1428.011 kb on + strand, within SM_b21503 at 1428.278 kb on - strand, within SM_b21503 at 1428.511 kb on + strand, within SM_b21503 at 1428.616 kb on + strand, within SM_b21503 at 1428.752 kb on - strand, within SM_b21503 at 1428.781 kb on - strand, within SM_b21503 at 1428.901 kb on + strand, within SM_b21503 at 1428.901 kb on + strand, within SM_b21503 at 1429.034 kb on - strand, within SM_b21503 at 1429.034 kb on - strand, within SM_b21503 at 1429.139 kb on + strand at 1429.139 kb on + strand at 1429.139 kb on + strand at 1429.140 kb on - strand at 1429.183 kb on + strand at 1429.183 kb on + strand at 1429.183 kb on + strand at 1429.184 kb on - strand at 1429.229 kb on + strand at 1429.292 kb on + strand at 1429.293 kb on - strand at 1429.331 kb on + strand, within SM_b21504 at 1429.332 kb on - strand, within SM_b21504 at 1429.332 kb on - strand, within SM_b21504 at 1429.566 kb on - strand, within SM_b21504 at 1429.784 kb on - strand, within SM_b21504 at 1429.784 kb on - strand, within SM_b21504 at 1429.784 kb on - strand, within SM_b21504 at 1429.785 kb on + strand, within SM_b21504 at 1429.787 kb on + strand, within SM_b21504 at 1429.787 kb on + strand, within SM_b21504 at 1429.787 kb on + strand, within SM_b21504 at 1429.787 kb on + strand, within SM_b21504 at 1429.787 kb on + strand, within SM_b21504 at 1429.788 kb on - strand, within SM_b21504 at 1429.788 kb on - strand, within SM_b21504 at 1429.788 kb on - strand, within SM_b21504 at 1429.788 kb on - strand, within SM_b21504 at 1429.788 kb on - strand, within SM_b21504 at 1429.788 kb on - strand, within SM_b21504 at 1429.808 kb on + strand, within SM_b21504 at 1429.808 kb on + strand, within SM_b21504 at 1429.809 kb on - strand, within SM_b21504 at 1429.809 kb on - strand, within SM_b21504 at 1429.809 kb on - strand, within SM_b21504 at 1429.988 kb on + strand at 1430.055 kb on + strand at 1430.056 kb on - strand at 1430.056 kb on - strand at 1430.137 kb on - strand, within SM_b21505 at 1430.145 kb on - strand, within SM_b21505 at 1430.150 kb on + strand, within SM_b21505 at 1430.151 kb on - strand, within SM_b21505
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,426,936 + SM_b21502 0.40 +0.2 1,426,937 - SM_b21502 0.40 -2.7 1,426,937 - SM_b21502 0.40 +0.2 1,426,996 + SM_b21502 0.45 +0.7 1,426,996 + SM_b21502 0.45 +3.5 1,426,997 - SM_b21502 0.45 +0.4 1,426,997 - SM_b21502 0.45 +1.6 1,427,016 + SM_b21502 0.46 +1.3 1,427,095 + SM_b21502 0.53 +0.6 1,427,095 + SM_b21502 0.53 +1.3 1,427,221 + SM_b21502 0.64 -1.6 1,427,221 + SM_b21502 0.64 +0.1 1,427,278 - SM_b21502 0.69 +1.4 1,427,338 + SM_b21502 0.74 -1.4 1,427,338 + SM_b21502 0.74 -1.7 1,427,339 - SM_b21502 0.74 +0.2 1,427,343 + SM_b21502 0.74 -0.3 1,427,343 + SM_b21502 0.74 +1.3 1,427,343 + SM_b21502 0.74 -0.7 1,427,343 + SM_b21502 0.74 +0.3 1,427,343 + SM_b21502 0.74 -2.5 1,427,343 + SM_b21502 0.74 -0.0 1,427,344 - SM_b21502 0.74 -0.9 1,427,344 - SM_b21502 0.74 +1.0 1,427,344 - SM_b21502 0.74 +1.2 1,427,357 + SM_b21502 0.76 +2.8 1,427,358 - SM_b21502 0.76 -0.9 1,427,358 - SM_b21502 0.76 -1.4 1,427,384 + SM_b21502 0.78 +1.4 1,427,384 + SM_b21502 0.78 -3.8 1,427,384 + SM_b21502 0.78 +1.0 1,427,384 + SM_b21502 0.78 +1.6 1,427,385 - SM_b21502 0.78 +0.4 1,427,385 - SM_b21502 0.78 +0.7 1,427,385 - SM_b21502 0.78 +0.7 1,427,385 - SM_b21502 0.78 -1.9 1,427,385 - SM_b21502 0.78 -0.3 1,427,385 - SM_b21502 0.78 -1.2 1,427,742 - -0.5 1,428,011 + SM_b21503 0.10 +1.6 1,428,278 - SM_b21503 0.30 -1.9 1,428,511 + SM_b21503 0.47 -2.0 1,428,616 + SM_b21503 0.55 +2.5 1,428,752 - SM_b21503 0.65 +0.7 1,428,781 - SM_b21503 0.67 -1.9 1,428,901 + SM_b21503 0.76 +1.5 1,428,901 + SM_b21503 0.76 +0.2 1,429,034 - SM_b21503 0.86 +1.7 1,429,034 - SM_b21503 0.86 +0.0 1,429,139 + -0.9 1,429,139 + -0.7 1,429,139 + +0.6 1,429,140 - +1.5 1,429,183 + -0.4 1,429,183 + +0.7 1,429,183 + +1.5 1,429,184 - -0.1 1,429,229 + -0.9 1,429,292 + -0.4 1,429,293 - +0.9 1,429,331 + SM_b21504 0.14 +1.5 1,429,332 - SM_b21504 0.14 +0.2 1,429,332 - SM_b21504 0.14 +0.9 1,429,566 - SM_b21504 0.45 -0.6 1,429,784 - SM_b21504 0.74 -1.0 1,429,784 - SM_b21504 0.74 -2.6 1,429,784 - SM_b21504 0.74 -1.9 1,429,785 + SM_b21504 0.74 +0.7 1,429,787 + SM_b21504 0.74 +0.3 1,429,787 + SM_b21504 0.74 +0.9 1,429,787 + SM_b21504 0.74 +0.9 1,429,787 + SM_b21504 0.74 -0.4 1,429,787 + SM_b21504 0.74 -1.6 1,429,788 - SM_b21504 0.74 -2.1 1,429,788 - SM_b21504 0.74 -1.7 1,429,788 - SM_b21504 0.74 +0.7 1,429,788 - SM_b21504 0.74 -0.5 1,429,788 - SM_b21504 0.74 -2.8 1,429,788 - SM_b21504 0.74 -1.1 1,429,808 + SM_b21504 0.77 +0.1 1,429,808 + SM_b21504 0.77 -0.9 1,429,809 - SM_b21504 0.77 -0.3 1,429,809 - SM_b21504 0.77 -1.4 1,429,809 - SM_b21504 0.77 +1.8 1,429,988 + -1.1 1,430,055 + -0.7 1,430,056 - -3.7 1,430,056 - -0.3 1,430,137 - SM_b21505 0.12 -0.6 1,430,145 - SM_b21505 0.13 +0.2 1,430,150 + SM_b21505 0.13 -1.1 1,430,151 - SM_b21505 0.13 +0.8
Or see this region's nucleotide sequence