Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21455 and SM_b21456 are separated by 283 nucleotides SM_b21456 and SM_b21458 are separated by 144 nucleotides SM_b21458 and SM_b21459 are separated by 11 nucleotides SM_b21459 and SM_b21460 overlap by 4 nucleotides
SM_b21455: SM_b21455 - hypothetical protein, at 1,382,140 to 1,383,507
_b21455
SM_b21456: SM_b21456 - hypothetical protein, at 1,383,791 to 1,384,228
_b21456
SM_b21458: SM_b21458 - sugar uptake ABC transporter permease, at 1,384,373 to 1,385,248
_b21458
SM_b21459: SM_b21459 - sugar uptake ABC transporter permease, at 1,385,260 to 1,386,153
_b21459
SM_b21460: SM_b21460 - sugar uptake ABC transporter ATP-binding protein, at 1,386,150 to 1,387,262
_b21460
Position (kb)
1384
1385
1386 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1383.374 kb on + strand at 1383.374 kb on + strand at 1383.374 kb on + strand at 1383.374 kb on + strand at 1383.375 kb on - strand at 1383.375 kb on - strand at 1383.375 kb on - strand at 1383.375 kb on - strand at 1383.375 kb on - strand at 1383.554 kb on + strand at 1383.554 kb on + strand at 1383.554 kb on + strand at 1383.560 kb on - strand at 1383.645 kb on - strand at 1383.645 kb on - strand at 1383.842 kb on + strand, within SM_b21456 at 1383.906 kb on + strand, within SM_b21456 at 1383.907 kb on - strand, within SM_b21456 at 1383.919 kb on + strand, within SM_b21456 at 1383.920 kb on - strand, within SM_b21456 at 1383.920 kb on - strand, within SM_b21456 at 1383.920 kb on - strand, within SM_b21456 at 1383.920 kb on - strand, within SM_b21456 at 1383.920 kb on - strand at 1383.923 kb on + strand, within SM_b21456 at 1383.923 kb on + strand, within SM_b21456 at 1383.924 kb on - strand, within SM_b21456 at 1383.924 kb on - strand, within SM_b21456 at 1383.966 kb on + strand, within SM_b21456 at 1383.966 kb on + strand, within SM_b21456 at 1383.966 kb on + strand, within SM_b21456 at 1384.071 kb on + strand, within SM_b21456 at 1384.071 kb on + strand, within SM_b21456 at 1384.072 kb on - strand, within SM_b21456 at 1384.072 kb on - strand, within SM_b21456 at 1384.203 kb on - strand at 1384.239 kb on + strand at 1384.250 kb on + strand at 1384.250 kb on + strand at 1384.276 kb on + strand at 1384.326 kb on + strand at 1384.327 kb on - strand at 1384.753 kb on + strand, within SM_b21458 at 1384.753 kb on + strand, within SM_b21458 at 1384.754 kb on - strand, within SM_b21458 at 1384.777 kb on - strand, within SM_b21458 at 1384.806 kb on + strand, within SM_b21458 at 1384.806 kb on + strand, within SM_b21458 at 1384.933 kb on - strand, within SM_b21458 at 1385.122 kb on - strand, within SM_b21458 at 1385.139 kb on + strand, within SM_b21458 at 1385.139 kb on + strand, within SM_b21458 at 1385.140 kb on - strand, within SM_b21458 at 1385.183 kb on + strand at 1385.183 kb on + strand at 1385.203 kb on - strand at 1385.394 kb on - strand, within SM_b21459 at 1385.438 kb on + strand, within SM_b21459 at 1385.439 kb on - strand, within SM_b21459 at 1385.521 kb on + strand, within SM_b21459 at 1385.906 kb on - strand, within SM_b21459 at 1386.103 kb on + strand at 1386.104 kb on - strand at 1386.104 kb on - strand at 1386.223 kb on + strand at 1386.223 kb on + strand at 1386.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,383,374 + -0.2 1,383,374 + +0.9 1,383,374 + +0.4 1,383,374 + -0.0 1,383,375 - +2.9 1,383,375 - -0.6 1,383,375 - +2.3 1,383,375 - -0.8 1,383,375 - -1.1 1,383,554 + +3.0 1,383,554 + -1.2 1,383,554 + -2.0 1,383,560 - +0.4 1,383,645 - -1.4 1,383,645 - -1.1 1,383,842 + SM_b21456 0.12 +0.1 1,383,906 + SM_b21456 0.26 -0.4 1,383,907 - SM_b21456 0.26 -1.9 1,383,919 + SM_b21456 0.29 -1.1 1,383,920 - SM_b21456 0.29 +0.5 1,383,920 - SM_b21456 0.29 -0.7 1,383,920 - SM_b21456 0.29 +0.5 1,383,920 - SM_b21456 0.29 -1.7 1,383,920 - -0.1 1,383,923 + SM_b21456 0.30 -1.1 1,383,923 + SM_b21456 0.30 -1.2 1,383,924 - SM_b21456 0.30 +1.5 1,383,924 - SM_b21456 0.30 -0.4 1,383,966 + SM_b21456 0.40 +2.2 1,383,966 + SM_b21456 0.40 -1.7 1,383,966 + SM_b21456 0.40 +0.0 1,384,071 + SM_b21456 0.64 -1.4 1,384,071 + SM_b21456 0.64 -3.1 1,384,072 - SM_b21456 0.64 +0.6 1,384,072 - SM_b21456 0.64 -1.0 1,384,203 - +0.2 1,384,239 + +4.4 1,384,250 + +0.7 1,384,250 + -1.1 1,384,276 + -1.4 1,384,326 + -0.7 1,384,327 - -2.7 1,384,753 + SM_b21458 0.43 -1.4 1,384,753 + SM_b21458 0.43 -0.7 1,384,754 - SM_b21458 0.43 +4.9 1,384,777 - SM_b21458 0.46 +0.4 1,384,806 + SM_b21458 0.49 +0.9 1,384,806 + SM_b21458 0.49 -1.1 1,384,933 - SM_b21458 0.64 -0.8 1,385,122 - SM_b21458 0.86 -0.6 1,385,139 + SM_b21458 0.87 +2.2 1,385,139 + SM_b21458 0.87 -0.0 1,385,140 - SM_b21458 0.88 -1.5 1,385,183 + +2.4 1,385,183 + +0.0 1,385,203 - +2.9 1,385,394 - SM_b21459 0.15 +0.6 1,385,438 + SM_b21459 0.20 -0.2 1,385,439 - SM_b21459 0.20 +0.8 1,385,521 + SM_b21459 0.29 -0.3 1,385,906 - SM_b21459 0.72 -3.0 1,386,103 + +1.2 1,386,104 - +0.8 1,386,104 - +0.6 1,386,223 + +0.6 1,386,223 + +1.4 1,386,224 - -2.2
Or see this region's nucleotide sequence