Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21418 and SM_b21419 are separated by 22 nucleotides SM_b21419 and SM_b21684 are separated by 21 nucleotides SM_b21684 and SM_b21420 overlap by 4 nucleotides
SM_b21418: SM_b21418 - NDP-hexose 3-C-methyltransferase, at 1,347,789 to 1,349,021
_b21418
SM_b21419: SM_b21419 - AraC family transcriptional regulator, at 1,349,044 to 1,349,931
_b21419
SM_b21684: SM_b21684 - hypothetical protein, at 1,349,953 to 1,350,180
_b21684
SM_b21420: SM_b21420 - L-arabinose isomerase, at 1,350,177 to 1,351,604
_b21420
Position (kb)
1349
1350 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1348.050 kb on + strand, within SM_b21418 at 1348.138 kb on - strand, within SM_b21418 at 1348.380 kb on - strand, within SM_b21418 at 1348.380 kb on - strand, within SM_b21418 at 1348.437 kb on + strand, within SM_b21418 at 1348.449 kb on + strand, within SM_b21418 at 1348.450 kb on - strand, within SM_b21418 at 1348.450 kb on - strand, within SM_b21418 at 1348.450 kb on - strand, within SM_b21418 at 1348.450 kb on - strand, within SM_b21418 at 1348.455 kb on + strand, within SM_b21418 at 1348.456 kb on - strand, within SM_b21418 at 1348.456 kb on - strand, within SM_b21418 at 1348.481 kb on + strand, within SM_b21418 at 1348.482 kb on - strand, within SM_b21418 at 1348.650 kb on + strand, within SM_b21418 at 1348.650 kb on + strand, within SM_b21418 at 1348.872 kb on + strand, within SM_b21418 at 1348.872 kb on + strand, within SM_b21418 at 1348.873 kb on - strand, within SM_b21418 at 1348.908 kb on + strand at 1348.908 kb on + strand at 1348.909 kb on - strand at 1348.909 kb on - strand at 1348.909 kb on - strand at 1348.962 kb on + strand at 1348.962 kb on + strand at 1348.963 kb on - strand at 1348.963 kb on - strand at 1348.963 kb on - strand at 1348.964 kb on + strand at 1349.076 kb on - strand at 1349.166 kb on + strand, within SM_b21419 at 1349.166 kb on + strand, within SM_b21419 at 1349.167 kb on - strand, within SM_b21419 at 1349.167 kb on - strand, within SM_b21419 at 1349.172 kb on + strand, within SM_b21419 at 1349.172 kb on + strand, within SM_b21419 at 1349.178 kb on + strand, within SM_b21419 at 1349.178 kb on + strand, within SM_b21419 at 1349.260 kb on + strand, within SM_b21419 at 1349.261 kb on - strand, within SM_b21419 at 1349.274 kb on - strand, within SM_b21419 at 1349.279 kb on + strand, within SM_b21419 at 1349.279 kb on + strand, within SM_b21419 at 1349.280 kb on - strand, within SM_b21419 at 1349.280 kb on - strand, within SM_b21419 at 1349.413 kb on + strand, within SM_b21419 at 1349.413 kb on + strand, within SM_b21419 at 1349.443 kb on + strand, within SM_b21419 at 1349.444 kb on - strand, within SM_b21419 at 1349.444 kb on - strand, within SM_b21419 at 1349.444 kb on - strand, within SM_b21419 at 1349.551 kb on + strand, within SM_b21419 at 1349.552 kb on - strand, within SM_b21419 at 1349.552 kb on - strand, within SM_b21419 at 1349.597 kb on - strand, within SM_b21419 at 1349.836 kb on + strand, within SM_b21419 at 1349.903 kb on + strand at 1350.010 kb on - strand, within SM_b21684 at 1350.093 kb on + strand, within SM_b21684 at 1350.093 kb on + strand, within SM_b21684 at 1350.093 kb on + strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.094 kb on - strand, within SM_b21684 at 1350.228 kb on + strand at 1350.229 kb on - strand at 1350.229 kb on - strand at 1350.270 kb on + strand at 1350.270 kb on + strand at 1350.271 kb on - strand at 1350.271 kb on - strand at 1350.271 kb on - strand at 1350.354 kb on + strand, within SM_b21420 at 1350.354 kb on + strand, within SM_b21420 at 1350.355 kb on - strand, within SM_b21420 at 1350.355 kb on - strand, within SM_b21420 at 1350.392 kb on + strand, within SM_b21420 at 1350.393 kb on - strand, within SM_b21420 at 1350.397 kb on + strand, within SM_b21420 at 1350.397 kb on + strand, within SM_b21420 at 1350.398 kb on - strand, within SM_b21420 at 1350.398 kb on - strand, within SM_b21420 at 1350.398 kb on - strand, within SM_b21420 at 1350.413 kb on + strand, within SM_b21420 at 1350.417 kb on + strand, within SM_b21420 at 1350.417 kb on + strand, within SM_b21420 at 1350.417 kb on + strand, within SM_b21420 at 1350.417 kb on + strand, within SM_b21420 at 1350.417 kb on + strand, within SM_b21420 at 1350.417 kb on + strand, within SM_b21420 at 1350.417 kb on + strand, within SM_b21420 at 1350.417 kb on + strand, within SM_b21420 at 1350.417 kb on + strand, within SM_b21420 at 1350.418 kb on - strand, within SM_b21420 at 1350.418 kb on - strand, within SM_b21420 at 1350.418 kb on - strand, within SM_b21420 at 1350.418 kb on - strand, within SM_b21420 at 1350.418 kb on - strand, within SM_b21420 at 1350.418 kb on - strand, within SM_b21420 at 1350.418 kb on - strand, within SM_b21420 at 1350.723 kb on - strand, within SM_b21420 at 1350.750 kb on + strand, within SM_b21420 at 1350.751 kb on - strand, within SM_b21420 at 1350.751 kb on - strand, within SM_b21420 at 1350.751 kb on - strand, within SM_b21420 at 1350.751 kb on - strand, within SM_b21420 at 1350.751 kb on - strand, within SM_b21420 at 1350.751 kb on - strand, within SM_b21420 at 1350.752 kb on + strand, within SM_b21420 at 1350.771 kb on + strand, within SM_b21420 at 1350.771 kb on + strand, within SM_b21420 at 1350.771 kb on + strand, within SM_b21420 at 1350.772 kb on - strand, within SM_b21420
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,348,050 + SM_b21418 0.21 +0.6 1,348,138 - SM_b21418 0.28 -2.1 1,348,380 - SM_b21418 0.48 -0.6 1,348,380 - SM_b21418 0.48 -3.8 1,348,437 + SM_b21418 0.53 -1.6 1,348,449 + SM_b21418 0.54 -2.3 1,348,450 - SM_b21418 0.54 -2.0 1,348,450 - SM_b21418 0.54 -1.4 1,348,450 - SM_b21418 0.54 -3.0 1,348,450 - SM_b21418 0.54 -4.0 1,348,455 + SM_b21418 0.54 +0.9 1,348,456 - SM_b21418 0.54 -1.4 1,348,456 - SM_b21418 0.54 -4.4 1,348,481 + SM_b21418 0.56 -0.5 1,348,482 - SM_b21418 0.56 -2.8 1,348,650 + SM_b21418 0.70 -2.6 1,348,650 + SM_b21418 0.70 -2.3 1,348,872 + SM_b21418 0.88 -1.4 1,348,872 + SM_b21418 0.88 -3.8 1,348,873 - SM_b21418 0.88 +0.7 1,348,908 + -1.5 1,348,908 + -1.9 1,348,909 - -4.9 1,348,909 - -3.1 1,348,909 - -4.0 1,348,962 + -2.3 1,348,962 + -0.7 1,348,963 - +0.9 1,348,963 - -1.9 1,348,963 - -3.6 1,348,964 + -1.5 1,349,076 - -1.7 1,349,166 + SM_b21419 0.14 +0.4 1,349,166 + SM_b21419 0.14 +0.3 1,349,167 - SM_b21419 0.14 -1.7 1,349,167 - SM_b21419 0.14 -1.4 1,349,172 + SM_b21419 0.14 -0.4 1,349,172 + SM_b21419 0.14 -1.9 1,349,178 + SM_b21419 0.15 -3.0 1,349,178 + SM_b21419 0.15 +1.5 1,349,260 + SM_b21419 0.24 +1.9 1,349,261 - SM_b21419 0.24 -0.2 1,349,274 - SM_b21419 0.26 +0.2 1,349,279 + SM_b21419 0.26 -1.4 1,349,279 + SM_b21419 0.26 -0.2 1,349,280 - SM_b21419 0.27 +0.3 1,349,280 - SM_b21419 0.27 -1.5 1,349,413 + SM_b21419 0.42 -1.4 1,349,413 + SM_b21419 0.42 -4.0 1,349,443 + SM_b21419 0.45 +0.7 1,349,444 - SM_b21419 0.45 -1.1 1,349,444 - SM_b21419 0.45 +0.2 1,349,444 - SM_b21419 0.45 +2.3 1,349,551 + SM_b21419 0.57 -1.1 1,349,552 - SM_b21419 0.57 -1.9 1,349,552 - SM_b21419 0.57 -0.3 1,349,597 - SM_b21419 0.62 +0.2 1,349,836 + SM_b21419 0.89 -0.3 1,349,903 + +0.2 1,350,010 - SM_b21684 0.25 +0.5 1,350,093 + SM_b21684 0.61 +0.0 1,350,093 + SM_b21684 0.61 +1.4 1,350,093 + SM_b21684 0.61 -1.3 1,350,094 - SM_b21684 0.62 +0.9 1,350,094 - SM_b21684 0.62 +1.2 1,350,094 - SM_b21684 0.62 +1.0 1,350,094 - SM_b21684 0.62 -0.8 1,350,094 - SM_b21684 0.62 +1.2 1,350,094 - SM_b21684 0.62 +1.6 1,350,094 - SM_b21684 0.62 +0.6 1,350,094 - SM_b21684 0.62 +0.4 1,350,094 - SM_b21684 0.62 -1.8 1,350,094 - SM_b21684 0.62 -0.1 1,350,228 + +0.4 1,350,229 - +1.2 1,350,229 - +1.5 1,350,270 + +0.1 1,350,270 + -0.3 1,350,271 - +1.5 1,350,271 - +0.8 1,350,271 - +0.3 1,350,354 + SM_b21420 0.12 -0.0 1,350,354 + SM_b21420 0.12 +0.2 1,350,355 - SM_b21420 0.12 -2.8 1,350,355 - SM_b21420 0.12 +1.0 1,350,392 + SM_b21420 0.15 +1.5 1,350,393 - SM_b21420 0.15 +0.9 1,350,397 + SM_b21420 0.15 -0.1 1,350,397 + SM_b21420 0.15 +0.4 1,350,398 - SM_b21420 0.15 -0.2 1,350,398 - SM_b21420 0.15 +0.4 1,350,398 - SM_b21420 0.15 -0.1 1,350,413 + SM_b21420 0.17 +2.2 1,350,417 + SM_b21420 0.17 +0.8 1,350,417 + SM_b21420 0.17 +0.5 1,350,417 + SM_b21420 0.17 +1.4 1,350,417 + SM_b21420 0.17 -0.5 1,350,417 + SM_b21420 0.17 +0.7 1,350,417 + SM_b21420 0.17 -2.1 1,350,417 + SM_b21420 0.17 +1.0 1,350,417 + SM_b21420 0.17 +2.6 1,350,417 + SM_b21420 0.17 +0.5 1,350,418 - SM_b21420 0.17 -0.4 1,350,418 - SM_b21420 0.17 -1.9 1,350,418 - SM_b21420 0.17 -0.2 1,350,418 - SM_b21420 0.17 -0.2 1,350,418 - SM_b21420 0.17 -0.9 1,350,418 - SM_b21420 0.17 -2.3 1,350,418 - SM_b21420 0.17 +0.6 1,350,723 - SM_b21420 0.38 +3.2 1,350,750 + SM_b21420 0.40 +1.9 1,350,751 - SM_b21420 0.40 -0.8 1,350,751 - SM_b21420 0.40 -0.7 1,350,751 - SM_b21420 0.40 +0.7 1,350,751 - SM_b21420 0.40 -0.8 1,350,751 - SM_b21420 0.40 -0.7 1,350,751 - SM_b21420 0.40 -0.5 1,350,752 + SM_b21420 0.40 -1.2 1,350,771 + SM_b21420 0.42 +0.1 1,350,771 + SM_b21420 0.42 +3.1 1,350,771 + SM_b21420 0.42 -0.2 1,350,772 - SM_b21420 0.42 -0.8
Or see this region's nucleotide sequence