Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21346 and SM_b21347 are separated by 72 nucleotides SM_b21347 and SM_b21348 are separated by 9 nucleotides SM_b21348 and SM_b21349 overlap by 4 nucleotides SM_b21349 and SM_b21350 are separated by 132 nucleotides
SM_b21346: SM_b21346 - LacI family transcriptional regulator, at 1,012,574 to 1,013,626
_b21346
SM_b21347: SM_b21347 - pectin degradation protein, at 1,013,699 to 1,014,037
_b21347
SM_b21348: SM_b21348 - 2-deoxy-D-gluconate 3-dehydrogenase, at 1,014,047 to 1,014,811
_b21348
SM_b21349: SM_b21349 - 5-keto-4-deoxyuronate isomerase, at 1,014,808 to 1,015,656
_b21349
SM_b21350: SM_b21350 - GntR family transcriptional regulator, at 1,015,789 to 1,016,538
_b21350
Position (kb)
1014
1015 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1013.255 kb on + strand, within SM_b21346 at 1013.396 kb on + strand, within SM_b21346 at 1013.397 kb on - strand, within SM_b21346 at 1013.397 kb on - strand, within SM_b21346 at 1013.421 kb on - strand, within SM_b21346 at 1013.459 kb on + strand, within SM_b21346 at 1013.557 kb on - strand at 1013.676 kb on + strand at 1013.700 kb on + strand at 1013.766 kb on - strand, within SM_b21347 at 1013.865 kb on + strand, within SM_b21347 at 1013.866 kb on - strand, within SM_b21347 at 1014.096 kb on + strand at 1014.097 kb on - strand at 1014.097 kb on - strand at 1014.097 kb on - strand at 1014.172 kb on + strand, within SM_b21348 at 1014.173 kb on - strand, within SM_b21348 at 1014.336 kb on + strand, within SM_b21348 at 1014.337 kb on - strand, within SM_b21348 at 1014.337 kb on - strand, within SM_b21348 at 1014.689 kb on + strand, within SM_b21348 at 1014.733 kb on + strand, within SM_b21348 at 1014.847 kb on - strand at 1014.847 kb on - strand at 1014.848 kb on + strand at 1014.849 kb on - strand at 1014.855 kb on + strand at 1014.856 kb on - strand at 1014.887 kb on + strand at 1014.887 kb on + strand at 1014.887 kb on + strand at 1014.888 kb on - strand at 1014.955 kb on + strand, within SM_b21349 at 1014.955 kb on + strand, within SM_b21349 at 1014.956 kb on - strand, within SM_b21349 at 1014.956 kb on - strand, within SM_b21349 at 1015.025 kb on + strand, within SM_b21349 at 1015.031 kb on + strand, within SM_b21349 at 1015.031 kb on + strand, within SM_b21349 at 1015.032 kb on - strand, within SM_b21349 at 1015.032 kb on - strand, within SM_b21349 at 1015.222 kb on - strand, within SM_b21349 at 1015.265 kb on + strand, within SM_b21349 at 1015.328 kb on + strand, within SM_b21349 at 1015.329 kb on - strand, within SM_b21349 at 1015.437 kb on + strand, within SM_b21349 at 1015.518 kb on + strand, within SM_b21349 at 1015.520 kb on + strand, within SM_b21349 at 1015.520 kb on + strand, within SM_b21349 at 1015.520 kb on + strand, within SM_b21349 at 1015.521 kb on - strand, within SM_b21349 at 1015.634 kb on + strand at 1015.696 kb on + strand at 1015.696 kb on + strand at 1015.697 kb on - strand at 1015.697 kb on - strand at 1015.700 kb on + strand at 1015.701 kb on - strand at 1015.701 kb on - strand at 1015.724 kb on + strand at 1015.725 kb on - strand at 1015.759 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,013,255 + SM_b21346 0.65 +0.9 1,013,396 + SM_b21346 0.78 -0.0 1,013,397 - SM_b21346 0.78 +0.6 1,013,397 - SM_b21346 0.78 +3.0 1,013,421 - SM_b21346 0.80 -2.6 1,013,459 + SM_b21346 0.84 +0.7 1,013,557 - -0.7 1,013,676 + -0.2 1,013,700 + -0.0 1,013,766 - SM_b21347 0.20 -1.1 1,013,865 + SM_b21347 0.49 +0.9 1,013,866 - SM_b21347 0.49 +2.2 1,014,096 + +2.1 1,014,097 - +2.5 1,014,097 - +0.1 1,014,097 - -2.5 1,014,172 + SM_b21348 0.16 -1.4 1,014,173 - SM_b21348 0.16 -4.0 1,014,336 + SM_b21348 0.38 -1.0 1,014,337 - SM_b21348 0.38 +0.7 1,014,337 - SM_b21348 0.38 -0.5 1,014,689 + SM_b21348 0.84 -0.9 1,014,733 + SM_b21348 0.90 -0.8 1,014,847 - -2.3 1,014,847 - -2.3 1,014,848 + +0.4 1,014,849 - -1.0 1,014,855 + -2.4 1,014,856 - -0.7 1,014,887 + -0.5 1,014,887 + +0.2 1,014,887 + +0.2 1,014,888 - +1.3 1,014,955 + SM_b21349 0.17 +0.2 1,014,955 + SM_b21349 0.17 +0.1 1,014,956 - SM_b21349 0.17 -1.4 1,014,956 - SM_b21349 0.17 +0.8 1,015,025 + SM_b21349 0.26 +0.6 1,015,031 + SM_b21349 0.26 +1.3 1,015,031 + SM_b21349 0.26 +0.3 1,015,032 - SM_b21349 0.26 +0.4 1,015,032 - SM_b21349 0.26 +1.4 1,015,222 - SM_b21349 0.49 -0.3 1,015,265 + SM_b21349 0.54 +0.9 1,015,328 + SM_b21349 0.61 -0.4 1,015,329 - SM_b21349 0.61 -0.8 1,015,437 + SM_b21349 0.74 -0.1 1,015,518 + SM_b21349 0.84 -1.2 1,015,520 + SM_b21349 0.84 +1.5 1,015,520 + SM_b21349 0.84 +0.3 1,015,520 + SM_b21349 0.84 -0.1 1,015,521 - SM_b21349 0.84 -2.2 1,015,634 + -0.9 1,015,696 + +0.7 1,015,696 + -3.5 1,015,697 - -0.1 1,015,697 - -2.4 1,015,700 + -2.1 1,015,701 - -1.4 1,015,701 - +0.8 1,015,724 + -0.3 1,015,725 - -2.6 1,015,759 - +1.2
Or see this region's nucleotide sequence