Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21324 and SM_b21325 overlap by 8 nucleotides SM_b21325 and SM_b21326 are separated by 11 nucleotides SM_b21326 and SM_b21327 overlap by 4 nucleotides SM_b21327 and SM_b21328 are separated by 45 nucleotides
SM_b21324: SM_b21324 - glucose-1-phosphate thymidylyltransferase, at 993,810 to 994,691
_b21324
SM_b21325: SM_b21325 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 994,684 to 995,247
_b21325
SM_b21326: SM_b21326 - dTDP-D-glucose 4,6-dehydratase, at 995,259 to 996,329
_b21326
SM_b21327: SM_b21327 - dTDP-4-dehydrorhamnose reductase, at 996,326 to 997,228
_b21327
SM_b21328: SM_b21328 - Sir2-like transcriptional silencer protein, at 997,274 to 998,092
_b21328
Position (kb)
995
996
997 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 994.422 kb on + strand, within SM_b21324 at 994.423 kb on - strand, within SM_b21324 at 994.423 kb on - strand, within SM_b21324 at 994.467 kb on + strand, within SM_b21324 at 994.482 kb on - strand, within SM_b21324 at 994.579 kb on + strand, within SM_b21324 at 994.580 kb on - strand, within SM_b21324 at 994.644 kb on + strand at 994.645 kb on - strand at 994.653 kb on + strand at 994.774 kb on + strand, within SM_b21325 at 994.775 kb on - strand, within SM_b21325 at 994.837 kb on + strand, within SM_b21325 at 994.873 kb on + strand, within SM_b21325 at 994.874 kb on - strand, within SM_b21325 at 994.874 kb on - strand, within SM_b21325 at 995.080 kb on + strand, within SM_b21325 at 995.088 kb on - strand, within SM_b21325 at 995.098 kb on + strand, within SM_b21325 at 995.150 kb on + strand, within SM_b21325 at 995.150 kb on + strand, within SM_b21325 at 995.151 kb on - strand, within SM_b21325 at 995.364 kb on + strand at 995.365 kb on - strand at 995.365 kb on - strand at 995.365 kb on - strand at 995.412 kb on + strand, within SM_b21326 at 995.413 kb on - strand, within SM_b21326 at 995.413 kb on - strand, within SM_b21326 at 995.413 kb on - strand, within SM_b21326 at 995.546 kb on + strand, within SM_b21326 at 995.716 kb on - strand, within SM_b21326 at 995.744 kb on + strand, within SM_b21326 at 995.779 kb on - strand, within SM_b21326 at 995.779 kb on - strand, within SM_b21326 at 995.787 kb on + strand, within SM_b21326 at 995.787 kb on + strand, within SM_b21326 at 995.787 kb on + strand, within SM_b21326 at 995.787 kb on + strand, within SM_b21326 at 995.787 kb on + strand, within SM_b21326 at 995.788 kb on + strand, within SM_b21326 at 995.788 kb on - strand, within SM_b21326 at 995.788 kb on - strand, within SM_b21326 at 995.788 kb on - strand, within SM_b21326 at 995.788 kb on - strand, within SM_b21326 at 995.788 kb on - strand, within SM_b21326 at 995.788 kb on - strand, within SM_b21326 at 995.827 kb on - strand, within SM_b21326 at 995.881 kb on + strand, within SM_b21326 at 995.882 kb on - strand, within SM_b21326 at 995.905 kb on - strand, within SM_b21326 at 995.905 kb on - strand, within SM_b21326 at 996.001 kb on - strand, within SM_b21326 at 996.001 kb on - strand, within SM_b21326 at 996.001 kb on - strand, within SM_b21326 at 996.184 kb on + strand, within SM_b21326 at 996.286 kb on - strand at 996.388 kb on + strand at 996.412 kb on + strand at 996.510 kb on - strand, within SM_b21327 at 996.510 kb on - strand, within SM_b21327 at 996.623 kb on - strand, within SM_b21327 at 996.635 kb on + strand, within SM_b21327 at 996.636 kb on - strand, within SM_b21327 at 996.636 kb on - strand, within SM_b21327 at 996.665 kb on + strand, within SM_b21327 at 996.666 kb on - strand, within SM_b21327 at 996.666 kb on - strand, within SM_b21327 at 996.666 kb on - strand, within SM_b21327 at 996.666 kb on - strand, within SM_b21327 at 996.694 kb on + strand, within SM_b21327 at 996.694 kb on + strand, within SM_b21327 at 996.783 kb on - strand, within SM_b21327 at 996.845 kb on - strand, within SM_b21327 at 996.913 kb on + strand, within SM_b21327 at 996.914 kb on - strand, within SM_b21327 at 996.988 kb on - strand, within SM_b21327 at 996.988 kb on - strand, within SM_b21327 at 997.227 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 994,422 + SM_b21324 0.69 -0.1 994,423 - SM_b21324 0.70 +0.0 994,423 - SM_b21324 0.70 +1.1 994,467 + SM_b21324 0.74 +0.4 994,482 - SM_b21324 0.76 -0.1 994,579 + SM_b21324 0.87 -1.5 994,580 - SM_b21324 0.87 -0.3 994,644 + +0.2 994,645 - +0.1 994,653 + +0.1 994,774 + SM_b21325 0.16 -0.4 994,775 - SM_b21325 0.16 -0.3 994,837 + SM_b21325 0.27 +0.7 994,873 + SM_b21325 0.34 -0.5 994,874 - SM_b21325 0.34 -0.3 994,874 - SM_b21325 0.34 +0.1 995,080 + SM_b21325 0.70 +0.7 995,088 - SM_b21325 0.72 -0.9 995,098 + SM_b21325 0.73 +1.0 995,150 + SM_b21325 0.83 -0.0 995,150 + SM_b21325 0.83 -0.2 995,151 - SM_b21325 0.83 +0.8 995,364 + -1.3 995,365 - -0.3 995,365 - +2.5 995,365 - -0.2 995,412 + SM_b21326 0.14 +0.9 995,413 - SM_b21326 0.14 +0.1 995,413 - SM_b21326 0.14 -0.1 995,413 - SM_b21326 0.14 -1.5 995,546 + SM_b21326 0.27 -1.7 995,716 - SM_b21326 0.43 -1.5 995,744 + SM_b21326 0.45 -1.4 995,779 - SM_b21326 0.49 +2.6 995,779 - SM_b21326 0.49 -1.1 995,787 + SM_b21326 0.49 +0.9 995,787 + SM_b21326 0.49 -0.6 995,787 + SM_b21326 0.49 -1.4 995,787 + SM_b21326 0.49 -0.7 995,787 + SM_b21326 0.49 -0.8 995,788 + SM_b21326 0.49 +0.3 995,788 - SM_b21326 0.49 +0.2 995,788 - SM_b21326 0.49 -0.4 995,788 - SM_b21326 0.49 -0.5 995,788 - SM_b21326 0.49 +4.3 995,788 - SM_b21326 0.49 -0.2 995,788 - SM_b21326 0.49 -1.5 995,827 - SM_b21326 0.53 -0.5 995,881 + SM_b21326 0.58 -2.5 995,882 - SM_b21326 0.58 +0.0 995,905 - SM_b21326 0.60 -0.7 995,905 - SM_b21326 0.60 +1.0 996,001 - SM_b21326 0.69 +0.7 996,001 - SM_b21326 0.69 -1.5 996,001 - SM_b21326 0.69 -3.0 996,184 + SM_b21326 0.86 -0.5 996,286 - +0.5 996,388 + +0.0 996,412 + -1.4 996,510 - SM_b21327 0.20 +0.6 996,510 - SM_b21327 0.20 +0.2 996,623 - SM_b21327 0.33 +0.1 996,635 + SM_b21327 0.34 +1.3 996,636 - SM_b21327 0.34 +0.3 996,636 - SM_b21327 0.34 +0.4 996,665 + SM_b21327 0.38 -2.1 996,666 - SM_b21327 0.38 -1.0 996,666 - SM_b21327 0.38 -1.9 996,666 - SM_b21327 0.38 -0.3 996,666 - SM_b21327 0.38 +0.6 996,694 + SM_b21327 0.41 +0.1 996,694 + SM_b21327 0.41 +1.0 996,783 - SM_b21327 0.51 -0.1 996,845 - SM_b21327 0.57 -3.3 996,913 + SM_b21327 0.65 +3.2 996,914 - SM_b21327 0.65 +0.3 996,988 - SM_b21327 0.73 -2.3 996,988 - SM_b21327 0.73 -2.7 997,227 - -1.7
Or see this region's nucleotide sequence