Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21311 and SM_b21312 overlap by 4 nucleotides SM_b21312 and SM_b21313 overlap by 4 nucleotides SM_b21313 and SM_b21314 are separated by 130 nucleotides
SM_b21311: SM_b21311 - glycosyltransferase, at 975,102 to 976,364
_b21311
SM_b21312: SM_b21312 - methyltransferase, at 976,361 to 977,206
_b21312
SM_b21313: SM_b21313 - bifunctional glycosyltransferase, at 977,203 to 979,452
_b21313
SM_b21314: SM_b21314 - secreted calcium-binding protein, at 979,583 to 980,242
_b21314
Position (kb) 977
978
979
980 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 976.273 kb on + strand at 976.274 kb on - strand at 976.334 kb on + strand at 976.335 kb on - strand at 976.404 kb on + strand at 976.404 kb on + strand at 976.405 kb on - strand at 976.536 kb on + strand, within SM_b21312 at 976.536 kb on + strand, within SM_b21312 at 976.536 kb on + strand, within SM_b21312 at 976.683 kb on + strand, within SM_b21312 at 976.684 kb on - strand, within SM_b21312 at 976.804 kb on - strand, within SM_b21312 at 976.804 kb on - strand, within SM_b21312 at 976.804 kb on - strand, within SM_b21312 at 976.911 kb on + strand, within SM_b21312 at 976.912 kb on - strand, within SM_b21312 at 977.089 kb on + strand, within SM_b21312 at 977.331 kb on - strand at 977.354 kb on + strand at 977.354 kb on + strand at 977.355 kb on - strand at 977.355 kb on - strand at 977.355 kb on - strand at 977.393 kb on + strand at 977.414 kb on + strand at 977.421 kb on + strand at 977.483 kb on + strand, within SM_b21313 at 977.484 kb on - strand, within SM_b21313 at 977.514 kb on - strand, within SM_b21313 at 977.514 kb on - strand, within SM_b21313 at 977.514 kb on - strand, within SM_b21313 at 977.585 kb on + strand, within SM_b21313 at 977.626 kb on + strand, within SM_b21313 at 977.627 kb on - strand, within SM_b21313 at 977.627 kb on - strand, within SM_b21313 at 977.954 kb on + strand, within SM_b21313 at 977.955 kb on - strand, within SM_b21313 at 977.955 kb on - strand, within SM_b21313 at 978.035 kb on + strand, within SM_b21313 at 978.036 kb on - strand, within SM_b21313 at 978.036 kb on - strand, within SM_b21313 at 978.095 kb on + strand, within SM_b21313 at 978.095 kb on + strand, within SM_b21313 at 978.096 kb on - strand, within SM_b21313 at 978.206 kb on - strand, within SM_b21313 at 978.404 kb on + strand at 978.450 kb on - strand, within SM_b21313 at 978.450 kb on - strand, within SM_b21313 at 978.548 kb on + strand, within SM_b21313 at 978.893 kb on + strand, within SM_b21313 at 978.893 kb on + strand, within SM_b21313 at 978.893 kb on + strand, within SM_b21313 at 978.893 kb on + strand, within SM_b21313 at 978.894 kb on - strand, within SM_b21313 at 978.894 kb on - strand, within SM_b21313 at 978.894 kb on - strand, within SM_b21313 at 978.894 kb on - strand, within SM_b21313 at 978.945 kb on - strand, within SM_b21313 at 979.097 kb on - strand, within SM_b21313 at 979.370 kb on - strand at 979.581 kb on + strand at 979.582 kb on - strand at 979.600 kb on - strand at 979.703 kb on - strand, within SM_b21314 at 979.747 kb on + strand, within SM_b21314 at 979.747 kb on + strand, within SM_b21314 at 979.757 kb on + strand, within SM_b21314 at 979.758 kb on - strand, within SM_b21314 at 979.832 kb on - strand, within SM_b21314 at 979.832 kb on - strand, within SM_b21314 at 979.987 kb on - strand, within SM_b21314 at 980.020 kb on + strand, within SM_b21314 at 980.020 kb on + strand, within SM_b21314 at 980.020 kb on + strand, within SM_b21314 at 980.040 kb on + strand, within SM_b21314 at 980.040 kb on + strand, within SM_b21314 at 980.041 kb on - strand, within SM_b21314 at 980.041 kb on - strand, within SM_b21314
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 976,273 + +0.6 976,274 - +0.9 976,334 + -0.1 976,335 - -0.4 976,404 + +1.3 976,404 + +0.1 976,405 - +0.9 976,536 + SM_b21312 0.21 +0.7 976,536 + SM_b21312 0.21 -0.6 976,536 + SM_b21312 0.21 +0.6 976,683 + SM_b21312 0.38 +0.1 976,684 - SM_b21312 0.38 +1.1 976,804 - SM_b21312 0.52 +0.0 976,804 - SM_b21312 0.52 +2.9 976,804 - SM_b21312 0.52 -0.3 976,911 + SM_b21312 0.65 +0.5 976,912 - SM_b21312 0.65 -0.7 977,089 + SM_b21312 0.86 -1.9 977,331 - -2.1 977,354 + -3.1 977,354 + +1.9 977,355 - +1.8 977,355 - -0.6 977,355 - +0.9 977,393 + -0.2 977,414 + -1.0 977,421 + +0.8 977,483 + SM_b21313 0.12 +1.0 977,484 - SM_b21313 0.12 -1.7 977,514 - SM_b21313 0.14 -0.2 977,514 - SM_b21313 0.14 -0.9 977,514 - SM_b21313 0.14 +0.8 977,585 + SM_b21313 0.17 +5.3 977,626 + SM_b21313 0.19 +0.6 977,627 - SM_b21313 0.19 -0.7 977,627 - SM_b21313 0.19 -0.2 977,954 + SM_b21313 0.33 +1.5 977,955 - SM_b21313 0.33 -0.2 977,955 - SM_b21313 0.33 -0.5 978,035 + SM_b21313 0.37 -1.7 978,036 - SM_b21313 0.37 -1.6 978,036 - SM_b21313 0.37 -0.9 978,095 + SM_b21313 0.40 -0.8 978,095 + SM_b21313 0.40 +1.5 978,096 - SM_b21313 0.40 -1.2 978,206 - SM_b21313 0.45 -1.1 978,404 + -0.7 978,450 - SM_b21313 0.55 -0.2 978,450 - SM_b21313 0.55 -1.1 978,548 + SM_b21313 0.60 +1.6 978,893 + SM_b21313 0.75 -0.7 978,893 + SM_b21313 0.75 +0.6 978,893 + SM_b21313 0.75 -0.0 978,893 + SM_b21313 0.75 -1.5 978,894 - SM_b21313 0.75 -1.4 978,894 - SM_b21313 0.75 +0.0 978,894 - SM_b21313 0.75 -0.8 978,894 - SM_b21313 0.75 -1.0 978,945 - SM_b21313 0.77 -0.5 979,097 - SM_b21313 0.84 +1.2 979,370 - -1.8 979,581 + -1.9 979,582 - +1.1 979,600 - +0.9 979,703 - SM_b21314 0.18 +0.6 979,747 + SM_b21314 0.25 +0.0 979,747 + SM_b21314 0.25 +0.2 979,757 + SM_b21314 0.26 -0.9 979,758 - SM_b21314 0.27 -0.5 979,832 - SM_b21314 0.38 +1.6 979,832 - SM_b21314 0.38 +0.1 979,987 - SM_b21314 0.61 -0.4 980,020 + SM_b21314 0.66 -2.0 980,020 + SM_b21314 0.66 +1.5 980,020 + SM_b21314 0.66 +0.6 980,040 + SM_b21314 0.69 +2.4 980,040 + SM_b21314 0.69 -0.7 980,041 - SM_b21314 0.69 -1.9 980,041 - SM_b21314 0.69 -0.9
Or see this region's nucleotide sequence