Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21225 and SM_b21226 are separated by 46 nucleotides SM_b21226 and SM_b21227 overlap by 4 nucleotides SM_b21227 and SM_b21228 are separated by 5 nucleotides
SM_b21225: SM_b21225 - inositol monophosphatase, at 789,434 to 790,273
_b21225
SM_b21226: SM_b21226 - hypothetical protein, at 790,320 to 791,060
_b21226
SM_b21227: SM_b21227 - carbamoyl phosphate synthase-like protein, at 791,057 to 792,049
_b21227
SM_b21228: SM_b21228 - nucleotide sugar epimerase dehydratase, at 792,055 to 792,990
_b21228
Position (kb) 791
792
793 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 790.259 kb on + strand at 790.260 kb on - strand at 790.339 kb on + strand at 790.339 kb on + strand at 790.340 kb on - strand at 790.340 kb on - strand at 790.507 kb on + strand, within SM_b21226 at 790.507 kb on + strand, within SM_b21226 at 790.508 kb on - strand, within SM_b21226 at 790.578 kb on - strand, within SM_b21226 at 790.578 kb on - strand, within SM_b21226 at 790.588 kb on - strand, within SM_b21226 at 790.857 kb on + strand, within SM_b21226 at 791.033 kb on - strand at 791.033 kb on - strand at 791.068 kb on + strand at 791.068 kb on + strand at 791.069 kb on - strand at 791.143 kb on - strand at 791.180 kb on + strand, within SM_b21227 at 791.198 kb on + strand, within SM_b21227 at 791.199 kb on - strand, within SM_b21227 at 791.229 kb on - strand, within SM_b21227 at 791.342 kb on + strand, within SM_b21227 at 791.343 kb on - strand at 791.439 kb on - strand, within SM_b21227 at 791.633 kb on + strand, within SM_b21227 at 791.633 kb on + strand, within SM_b21227 at 791.710 kb on + strand, within SM_b21227 at 791.710 kb on + strand, within SM_b21227 at 791.711 kb on - strand, within SM_b21227 at 791.714 kb on + strand, within SM_b21227 at 791.714 kb on + strand, within SM_b21227 at 791.715 kb on - strand, within SM_b21227 at 791.715 kb on - strand, within SM_b21227 at 791.786 kb on + strand, within SM_b21227 at 791.787 kb on - strand, within SM_b21227 at 791.787 kb on - strand, within SM_b21227 at 791.970 kb on + strand at 791.971 kb on - strand at 791.971 kb on - strand at 792.127 kb on + strand at 792.128 kb on - strand at 792.266 kb on - strand, within SM_b21228 at 792.404 kb on - strand, within SM_b21228 at 792.404 kb on - strand, within SM_b21228 at 792.404 kb on - strand, within SM_b21228 at 792.466 kb on + strand, within SM_b21228 at 792.467 kb on - strand, within SM_b21228 at 792.467 kb on - strand, within SM_b21228 at 792.467 kb on - strand, within SM_b21228 at 792.470 kb on - strand, within SM_b21228 at 792.562 kb on + strand, within SM_b21228 at 792.563 kb on - strand, within SM_b21228 at 792.563 kb on - strand, within SM_b21228 at 792.563 kb on - strand, within SM_b21228 at 792.563 kb on - strand, within SM_b21228 at 792.563 kb on - strand, within SM_b21228 at 792.655 kb on + strand, within SM_b21228 at 792.655 kb on + strand, within SM_b21228 at 792.668 kb on + strand, within SM_b21228 at 792.668 kb on + strand, within SM_b21228 at 792.673 kb on + strand, within SM_b21228 at 792.674 kb on - strand, within SM_b21228 at 792.731 kb on + strand, within SM_b21228 at 792.901 kb on + strand at 792.988 kb on + strand at 792.988 kb on + strand at 792.988 kb on + strand at 792.989 kb on - strand at 793.039 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 790,259 + +1.2 790,260 - +1.7 790,339 + -0.7 790,339 + -0.7 790,340 - -2.2 790,340 - -2.0 790,507 + SM_b21226 0.25 +0.3 790,507 + SM_b21226 0.25 +0.3 790,508 - SM_b21226 0.25 +1.4 790,578 - SM_b21226 0.35 -2.2 790,578 - SM_b21226 0.35 -0.2 790,588 - SM_b21226 0.36 -2.2 790,857 + SM_b21226 0.72 +0.5 791,033 - +0.8 791,033 - +0.8 791,068 + -0.3 791,068 + -0.2 791,069 - +1.1 791,143 - -1.1 791,180 + SM_b21227 0.12 -1.2 791,198 + SM_b21227 0.14 -2.0 791,199 - SM_b21227 0.14 +3.6 791,229 - SM_b21227 0.17 +0.1 791,342 + SM_b21227 0.29 -0.6 791,343 - -1.5 791,439 - SM_b21227 0.38 -1.5 791,633 + SM_b21227 0.58 +0.2 791,633 + SM_b21227 0.58 -1.1 791,710 + SM_b21227 0.66 -1.2 791,710 + SM_b21227 0.66 -1.7 791,711 - SM_b21227 0.66 -0.9 791,714 + SM_b21227 0.66 -0.0 791,714 + SM_b21227 0.66 -0.7 791,715 - SM_b21227 0.66 +0.6 791,715 - SM_b21227 0.66 -2.0 791,786 + SM_b21227 0.73 -2.2 791,787 - SM_b21227 0.74 -0.3 791,787 - SM_b21227 0.74 +0.8 791,970 + -0.2 791,971 - +0.3 791,971 - +0.8 792,127 + +0.2 792,128 - +0.0 792,266 - SM_b21228 0.23 -0.9 792,404 - SM_b21228 0.37 +0.8 792,404 - SM_b21228 0.37 -1.5 792,404 - SM_b21228 0.37 -0.2 792,466 + SM_b21228 0.44 -1.2 792,467 - SM_b21228 0.44 -0.5 792,467 - SM_b21228 0.44 +0.8 792,467 - SM_b21228 0.44 +0.7 792,470 - SM_b21228 0.44 +0.3 792,562 + SM_b21228 0.54 +0.1 792,563 - SM_b21228 0.54 -3.2 792,563 - SM_b21228 0.54 +0.4 792,563 - SM_b21228 0.54 +0.2 792,563 - SM_b21228 0.54 -0.7 792,563 - SM_b21228 0.54 -2.2 792,655 + SM_b21228 0.64 +0.2 792,655 + SM_b21228 0.64 -2.6 792,668 + SM_b21228 0.65 -0.4 792,668 + SM_b21228 0.65 +0.2 792,673 + SM_b21228 0.66 -0.0 792,674 - SM_b21228 0.66 +3.1 792,731 + SM_b21228 0.72 -0.9 792,901 + +2.2 792,988 + +0.2 792,988 + -0.8 792,988 + -4.9 792,989 - +1.7 793,039 + -0.1
Or see this region's nucleotide sequence