Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21667 and SM_b21213 overlap by 8 nucleotides SM_b21213 and SM_b21214 are separated by 128 nucleotides SM_b21214 and SM_b21215 are separated by 67 nucleotides
SM_b21667: SM_b21667 - hypothetical protein, at 966,345 to 966,524
_b21667
SM_b21213: SM_b21213 - serinethreonine protein phosphatase, at 966,517 to 967,176
_b21213
SM_b21214: SM_b21214 - 2-deoxyglucose-6-phosphate phosphatase, at 967,305 to 968,027
_b21214
SM_b21215: SM_b21215 - dipeptide uptake ABC transporter substrate-binding protein precursor, at 968,095 to 969,702
_b21215
Position (kb) 967
968
969 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 966.364 kb on - strand, within SM_b21667 at 966.372 kb on + strand, within SM_b21667 at 966.421 kb on + strand, within SM_b21667 at 966.422 kb on - strand, within SM_b21667 at 966.464 kb on + strand at 966.998 kb on - strand, within SM_b21213 at 966.998 kb on - strand, within SM_b21213 at 966.998 kb on - strand, within SM_b21213 at 966.998 kb on - strand, within SM_b21213 at 966.998 kb on - strand, within SM_b21213 at 967.026 kb on + strand, within SM_b21213 at 967.169 kb on - strand at 967.169 kb on - strand at 967.174 kb on + strand at 967.175 kb on - strand at 967.175 kb on - strand at 967.194 kb on + strand at 967.194 kb on + strand at 967.195 kb on - strand at 967.195 kb on - strand at 967.275 kb on - strand at 967.303 kb on - strand at 967.303 kb on - strand at 967.303 kb on - strand at 967.423 kb on + strand, within SM_b21214 at 967.424 kb on - strand, within SM_b21214 at 967.483 kb on + strand, within SM_b21214 at 967.484 kb on - strand, within SM_b21214 at 967.484 kb on - strand, within SM_b21214 at 967.484 kb on - strand, within SM_b21214 at 967.731 kb on - strand, within SM_b21214 at 967.731 kb on - strand, within SM_b21214 at 967.797 kb on + strand, within SM_b21214 at 967.946 kb on + strand, within SM_b21214 at 967.946 kb on + strand, within SM_b21214 at 968.048 kb on + strand at 968.048 kb on + strand at 968.049 kb on - strand at 968.193 kb on + strand at 968.194 kb on - strand at 968.194 kb on - strand at 968.195 kb on + strand at 968.195 kb on + strand at 968.195 kb on + strand at 968.195 kb on + strand at 968.196 kb on - strand at 968.196 kb on - strand at 968.196 kb on - strand at 968.196 kb on - strand at 968.196 kb on - strand at 968.196 kb on - strand at 968.196 kb on - strand at 968.367 kb on - strand, within SM_b21215 at 968.378 kb on + strand, within SM_b21215 at 968.411 kb on + strand, within SM_b21215 at 968.411 kb on + strand, within SM_b21215 at 968.454 kb on - strand, within SM_b21215 at 968.683 kb on - strand, within SM_b21215 at 968.813 kb on + strand, within SM_b21215 at 968.813 kb on + strand, within SM_b21215 at 968.813 kb on + strand, within SM_b21215 at 968.813 kb on + strand, within SM_b21215 at 968.814 kb on - strand, within SM_b21215 at 968.814 kb on - strand, within SM_b21215 at 968.814 kb on - strand, within SM_b21215 at 968.814 kb on - strand, within SM_b21215 at 968.814 kb on - strand, within SM_b21215 at 969.002 kb on + strand, within SM_b21215
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 966,364 - SM_b21667 0.11 -2.7 966,372 + SM_b21667 0.15 +2.5 966,421 + SM_b21667 0.42 +0.5 966,422 - SM_b21667 0.43 -0.0 966,464 + +0.9 966,998 - SM_b21213 0.73 -1.4 966,998 - SM_b21213 0.73 -2.6 966,998 - SM_b21213 0.73 -3.7 966,998 - SM_b21213 0.73 -0.4 966,998 - SM_b21213 0.73 -1.2 967,026 + SM_b21213 0.77 -0.7 967,169 - -0.1 967,169 - -1.0 967,174 + -2.1 967,175 - -0.4 967,175 - -2.1 967,194 + +1.4 967,194 + -1.1 967,195 - +0.5 967,195 - -0.2 967,275 - -1.9 967,303 - +1.7 967,303 - +0.3 967,303 - +0.0 967,423 + SM_b21214 0.16 -3.1 967,424 - SM_b21214 0.16 +1.4 967,483 + SM_b21214 0.25 -0.6 967,484 - SM_b21214 0.25 -1.8 967,484 - SM_b21214 0.25 -1.4 967,484 - SM_b21214 0.25 +0.0 967,731 - SM_b21214 0.59 -0.3 967,731 - SM_b21214 0.59 -1.9 967,797 + SM_b21214 0.68 +0.2 967,946 + SM_b21214 0.89 -2.8 967,946 + SM_b21214 0.89 +0.4 968,048 + -1.8 968,048 + +0.9 968,049 - +0.1 968,193 + +0.9 968,194 - +0.1 968,194 - -0.7 968,195 + +0.7 968,195 + +0.7 968,195 + -0.9 968,195 + -0.1 968,196 - -0.6 968,196 - +0.5 968,196 - -1.0 968,196 - +0.7 968,196 - +0.0 968,196 - -0.5 968,196 - -0.1 968,367 - SM_b21215 0.17 +0.3 968,378 + SM_b21215 0.18 +1.2 968,411 + SM_b21215 0.20 -0.5 968,411 + SM_b21215 0.20 -3.1 968,454 - SM_b21215 0.22 -0.0 968,683 - SM_b21215 0.37 +0.1 968,813 + SM_b21215 0.45 -0.1 968,813 + SM_b21215 0.45 +0.5 968,813 + SM_b21215 0.45 +0.6 968,813 + SM_b21215 0.45 -0.3 968,814 - SM_b21215 0.45 -2.2 968,814 - SM_b21215 0.45 +0.9 968,814 - SM_b21215 0.45 -1.3 968,814 - SM_b21215 0.45 -1.7 968,814 - SM_b21215 0.45 -4.1 969,002 + SM_b21215 0.56 -0.5
Or see this region's nucleotide sequence