Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21124 and SM_b21125 overlap by 4 nucleotides SM_b21125 and SM_b21126 overlap by 4 nucleotides SM_b21126 and SM_b21127 are separated by 76 nucleotides SM_b21127 and SM_b21128 are separated by 6 nucleotides
SM_b21124: SM_b21124 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) <small>(from data)</small>, at 762,782 to 764,770
_b21124
SM_b21125: SM_b21125 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) <small>(from data)</small>, at 764,767 to 765,636
_b21125
SM_b21126: SM_b21126 - 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18) <small>(from data)</small>, at 765,633 to 766,418
_b21126
SM_b21127: SM_b21127 - hypothetical protein, at 766,495 to 766,866
_b21127
SM_b21128: SM_b21128 - hypothetical protein, at 766,873 to 767,427
_b21128
Position (kb) 765
766
767 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 765.021 kb on + strand, within SM_b21125 at 765.021 kb on + strand, within SM_b21125 at 765.193 kb on + strand, within SM_b21125 at 765.193 kb on + strand, within SM_b21125 at 765.193 kb on + strand, within SM_b21125 at 765.194 kb on - strand, within SM_b21125 at 765.221 kb on - strand, within SM_b21125 at 765.221 kb on - strand, within SM_b21125 at 765.287 kb on - strand, within SM_b21125 at 765.402 kb on + strand, within SM_b21125 at 765.402 kb on + strand, within SM_b21125 at 765.403 kb on - strand, within SM_b21125 at 765.496 kb on + strand, within SM_b21125 at 765.526 kb on - strand, within SM_b21125 at 766.080 kb on + strand, within SM_b21126 at 766.081 kb on - strand, within SM_b21126 at 766.233 kb on + strand, within SM_b21126 at 766.233 kb on + strand, within SM_b21126 at 766.234 kb on - strand, within SM_b21126 at 766.327 kb on + strand, within SM_b21126 at 766.864 kb on + strand at 766.864 kb on + strand at 766.865 kb on - strand at 766.865 kb on - strand at 766.974 kb on + strand, within SM_b21128 at 766.974 kb on + strand, within SM_b21128 at 766.974 kb on + strand, within SM_b21128 at 766.974 kb on + strand, within SM_b21128 at 766.974 kb on + strand, within SM_b21128 at 766.975 kb on - strand, within SM_b21128 at 766.975 kb on - strand, within SM_b21128 at 766.975 kb on - strand, within SM_b21128 at 766.975 kb on - strand, within SM_b21128 at 767.081 kb on - strand, within SM_b21128 at 767.082 kb on + strand, within SM_b21128 at 767.083 kb on - strand, within SM_b21128 at 767.083 kb on - strand, within SM_b21128 at 767.284 kb on + strand, within SM_b21128 at 767.285 kb on - strand, within SM_b21128 at 767.285 kb on - strand, within SM_b21128 at 767.285 kb on - strand, within SM_b21128 at 767.299 kb on + strand, within SM_b21128 at 767.299 kb on + strand, within SM_b21128 at 767.299 kb on + strand, within SM_b21128 at 767.300 kb on - strand, within SM_b21128
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 765,021 + SM_b21125 0.29 +1.9 765,021 + SM_b21125 0.29 +0.6 765,193 + SM_b21125 0.49 -1.2 765,193 + SM_b21125 0.49 -0.8 765,193 + SM_b21125 0.49 +0.2 765,194 - SM_b21125 0.49 -0.8 765,221 - SM_b21125 0.52 +2.2 765,221 - SM_b21125 0.52 +1.1 765,287 - SM_b21125 0.60 -1.1 765,402 + SM_b21125 0.73 +1.8 765,402 + SM_b21125 0.73 +1.4 765,403 - SM_b21125 0.73 +1.7 765,496 + SM_b21125 0.84 -0.5 765,526 - SM_b21125 0.87 +1.2 766,080 + SM_b21126 0.57 +1.3 766,081 - SM_b21126 0.57 -0.3 766,233 + SM_b21126 0.76 -2.1 766,233 + SM_b21126 0.76 -0.1 766,234 - SM_b21126 0.76 -2.0 766,327 + SM_b21126 0.88 +0.3 766,864 + +0.0 766,864 + -2.3 766,865 - -0.4 766,865 - -0.8 766,974 + SM_b21128 0.18 -0.8 766,974 + SM_b21128 0.18 +0.8 766,974 + SM_b21128 0.18 +1.3 766,974 + SM_b21128 0.18 -0.1 766,974 + SM_b21128 0.18 -0.2 766,975 - SM_b21128 0.18 +2.4 766,975 - SM_b21128 0.18 -2.8 766,975 - SM_b21128 0.18 -0.0 766,975 - SM_b21128 0.18 +0.1 767,081 - SM_b21128 0.37 -0.4 767,082 + SM_b21128 0.38 +1.0 767,083 - SM_b21128 0.38 +1.1 767,083 - SM_b21128 0.38 -1.2 767,284 + SM_b21128 0.74 -1.2 767,285 - SM_b21128 0.74 -4.3 767,285 - SM_b21128 0.74 -0.3 767,285 - SM_b21128 0.74 +0.9 767,299 + SM_b21128 0.77 +2.6 767,299 + SM_b21128 0.77 +0.9 767,299 + SM_b21128 0.77 -1.5 767,300 - SM_b21128 0.77 -0.2
Or see this region's nucleotide sequence