Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21121 and SM_b21122 are separated by 27 nucleotides SM_b21122 and SM_b21123 are separated by 14 nucleotides SM_b21123 and SM_b21124 are separated by 16 nucleotides
SM_b21121: SM_b21121 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (from data) , at 759,569 to 760,732
_b21121
SM_b21122: SM_b21122 - 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4) (from data) , at 760,760 to 762,367
_b21122
SM_b21123: SM_b21123 - translation initiation inhibitor, at 762,382 to 762,765
_b21123
SM_b21124: SM_b21124 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 762,782 to 764,770
_b21124
Position (kb)
760
761
762
763 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 759.811 kb on - strand, within SM_b21121 at 759.811 kb on - strand, within SM_b21121 at 759.811 kb on - strand, within SM_b21121 at 759.843 kb on - strand, within SM_b21121 at 759.911 kb on + strand, within SM_b21121 at 759.911 kb on + strand, within SM_b21121 at 759.911 kb on + strand, within SM_b21121 at 759.912 kb on - strand, within SM_b21121 at 760.025 kb on + strand, within SM_b21121 at 760.026 kb on - strand, within SM_b21121 at 760.088 kb on + strand, within SM_b21121 at 760.088 kb on + strand, within SM_b21121 at 760.089 kb on - strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.289 kb on - strand, within SM_b21121 at 760.289 kb on - strand, within SM_b21121 at 760.374 kb on - strand, within SM_b21121 at 760.374 kb on - strand, within SM_b21121 at 760.374 kb on - strand, within SM_b21121 at 760.448 kb on + strand at 760.448 kb on + strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.475 kb on + strand, within SM_b21121 at 760.475 kb on + strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.481 kb on + strand, within SM_b21121 at 760.540 kb on + strand, within SM_b21121 at 760.588 kb on - strand, within SM_b21121 at 760.588 kb on - strand, within SM_b21121 at 760.611 kb on - strand, within SM_b21121 at 760.658 kb on + strand at 760.740 kb on - strand at 760.743 kb on + strand at 760.768 kb on - strand at 760.942 kb on + strand, within SM_b21122 at 760.994 kb on + strand, within SM_b21122 at 761.003 kb on + strand, within SM_b21122 at 761.003 kb on + strand, within SM_b21122 at 761.004 kb on - strand, within SM_b21122 at 761.004 kb on - strand, within SM_b21122 at 761.004 kb on - strand, within SM_b21122 at 761.004 kb on - strand, within SM_b21122 at 761.108 kb on + strand, within SM_b21122 at 761.108 kb on + strand, within SM_b21122 at 761.109 kb on - strand, within SM_b21122 at 761.112 kb on - strand, within SM_b21122 at 761.180 kb on + strand, within SM_b21122 at 761.180 kb on + strand, within SM_b21122 at 761.180 kb on + strand, within SM_b21122 at 761.181 kb on - strand, within SM_b21122 at 761.181 kb on - strand, within SM_b21122 at 761.262 kb on - strand, within SM_b21122 at 761.262 kb on - strand, within SM_b21122 at 761.263 kb on + strand, within SM_b21122 at 761.339 kb on + strand, within SM_b21122 at 761.339 kb on + strand, within SM_b21122 at 761.340 kb on - strand, within SM_b21122 at 761.340 kb on - strand, within SM_b21122 at 761.390 kb on - strand, within SM_b21122 at 761.609 kb on + strand, within SM_b21122 at 761.660 kb on + strand, within SM_b21122 at 761.661 kb on - strand, within SM_b21122 at 761.912 kb on + strand, within SM_b21122 at 762.023 kb on + strand, within SM_b21122 at 762.210 kb on + strand at 762.211 kb on - strand at 762.236 kb on + strand at 762.237 kb on - strand at 762.237 kb on - strand at 762.497 kb on + strand, within SM_b21123 at 762.670 kb on + strand, within SM_b21123 at 762.670 kb on + strand, within SM_b21123 at 762.671 kb on - strand, within SM_b21123 at 762.763 kb on + strand at 762.763 kb on + strand at 762.764 kb on - strand at 762.782 kb on - strand at 762.782 kb on - strand at 762.959 kb on + strand at 762.977 kb on - strand at 763.083 kb on + strand, within SM_b21124 at 763.083 kb on + strand, within SM_b21124 at 763.083 kb on + strand, within SM_b21124 at 763.084 kb on - strand, within SM_b21124 at 763.084 kb on - strand, within SM_b21124
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 759,811 - SM_b21121 0.21 +0.6 759,811 - SM_b21121 0.21 +1.1 759,811 - SM_b21121 0.21 -0.7 759,843 - SM_b21121 0.24 -0.2 759,911 + SM_b21121 0.29 -1.1 759,911 + SM_b21121 0.29 -0.7 759,911 + SM_b21121 0.29 +0.8 759,912 - SM_b21121 0.29 +0.0 760,025 + SM_b21121 0.39 -0.0 760,026 - SM_b21121 0.39 -1.0 760,088 + SM_b21121 0.45 -3.2 760,088 + SM_b21121 0.45 -2.7 760,089 - SM_b21121 0.45 +0.6 760,288 + SM_b21121 0.62 -2.6 760,288 + SM_b21121 0.62 -1.3 760,288 + SM_b21121 0.62 +0.7 760,288 + SM_b21121 0.62 +1.0 760,288 + SM_b21121 0.62 -0.4 760,289 - SM_b21121 0.62 +0.2 760,289 - SM_b21121 0.62 -0.4 760,374 - SM_b21121 0.69 -1.5 760,374 - SM_b21121 0.69 +1.0 760,374 - SM_b21121 0.69 +0.5 760,448 + -1.5 760,448 + SM_b21121 0.76 +2.7 760,449 - SM_b21121 0.76 -0.3 760,449 - SM_b21121 0.76 +0.2 760,449 - SM_b21121 0.76 +0.1 760,449 - SM_b21121 0.76 +1.2 760,449 - SM_b21121 0.76 -0.5 760,449 - SM_b21121 0.76 +0.0 760,475 + SM_b21121 0.78 -1.6 760,475 + SM_b21121 0.78 -1.5 760,476 - SM_b21121 0.78 +0.6 760,476 - SM_b21121 0.78 -1.1 760,476 - SM_b21121 0.78 -2.3 760,476 - SM_b21121 0.78 +1.1 760,481 + SM_b21121 0.78 +0.3 760,540 + SM_b21121 0.83 -1.8 760,588 - SM_b21121 0.88 +0.5 760,588 - SM_b21121 0.88 -0.7 760,611 - SM_b21121 0.90 -3.2 760,658 + -0.9 760,740 - -2.3 760,743 + -0.5 760,768 - +0.5 760,942 + SM_b21122 0.11 -2.0 760,994 + SM_b21122 0.15 -1.5 761,003 + SM_b21122 0.15 +1.1 761,003 + SM_b21122 0.15 -1.7 761,004 - SM_b21122 0.15 -3.5 761,004 - SM_b21122 0.15 -2.5 761,004 - SM_b21122 0.15 +0.4 761,004 - SM_b21122 0.15 -0.4 761,108 + SM_b21122 0.22 +0.4 761,108 + SM_b21122 0.22 +0.3 761,109 - SM_b21122 0.22 +1.3 761,112 - SM_b21122 0.22 +1.4 761,180 + SM_b21122 0.26 +0.7 761,180 + SM_b21122 0.26 +1.4 761,180 + SM_b21122 0.26 +0.7 761,181 - SM_b21122 0.26 +0.5 761,181 - SM_b21122 0.26 -1.3 761,262 - SM_b21122 0.31 -1.0 761,262 - SM_b21122 0.31 -1.2 761,263 + SM_b21122 0.31 -0.5 761,339 + SM_b21122 0.36 -3.8 761,339 + SM_b21122 0.36 -1.3 761,340 - SM_b21122 0.36 +3.2 761,340 - SM_b21122 0.36 -0.9 761,390 - SM_b21122 0.39 -0.0 761,609 + SM_b21122 0.53 +4.6 761,660 + SM_b21122 0.56 -0.4 761,661 - SM_b21122 0.56 -0.0 761,912 + SM_b21122 0.72 -3.3 762,023 + SM_b21122 0.79 -0.1 762,210 + -0.0 762,211 - +0.2 762,236 + -2.3 762,237 - -1.5 762,237 - -3.0 762,497 + SM_b21123 0.30 +2.5 762,670 + SM_b21123 0.75 -0.2 762,670 + SM_b21123 0.75 -0.9 762,671 - SM_b21123 0.75 -0.5 762,763 + +1.4 762,763 + +2.5 762,764 - +0.1 762,782 - +1.9 762,782 - -1.0 762,959 + +0.7 762,977 - +0.8 763,083 + SM_b21124 0.15 -1.5 763,083 + SM_b21124 0.15 -1.5 763,083 + SM_b21124 0.15 -1.0 763,084 - SM_b21124 0.15 +0.8 763,084 - SM_b21124 0.15 +0.8
Or see this region's nucleotide sequence