Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21109 and SM_b21110 are separated by 1 nucleotides SM_b21110 and SM_b21111 overlap by 4 nucleotides SM_b21111 and SM_b21112 are separated by 22 nucleotides SM_b21112 and SM_b21113 are separated by 123 nucleotides
SM_b21109: SM_b21109 - L-fucose dehydrogenase (EC 1.1.1.122) (from data) , at 750,677 to 751,759
_b21109
SM_b21110: SM_b21110 - dehydratase involved in L-fucose catabolism (from data) , at 751,761 to 752,222
_b21110
SM_b21111: SM_b21111 - oxidoreductase, RED superfamily protein, at 752,219 to 752,953
_b21111
SM_b21112: SM_b21112 - L-2,4-diketo-3-deoxyrhamnonate hydrolase; 2,4-dioxopentanoate hydrolase (from data) , at 752,976 to 753,821
_b21112
SM_b21113: SM_b21113 - hypothetical protein, at 753,945 to 755,222
_b21113
Position (kb)
752
753 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 751.256 kb on + strand, within SM_b21109 at 751.256 kb on + strand, within SM_b21109 at 751.256 kb on + strand, within SM_b21109 at 751.257 kb on - strand, within SM_b21109 at 751.472 kb on + strand, within SM_b21109 at 751.473 kb on - strand, within SM_b21109 at 751.473 kb on - strand, within SM_b21109 at 751.473 kb on - strand, within SM_b21109 at 751.779 kb on + strand at 751.783 kb on - strand at 751.783 kb on - strand at 751.783 kb on - strand at 751.783 kb on - strand at 751.791 kb on + strand at 751.791 kb on + strand at 752.008 kb on - strand, within SM_b21110 at 752.008 kb on - strand, within SM_b21110 at 752.054 kb on + strand, within SM_b21110 at 752.055 kb on - strand, within SM_b21110 at 752.213 kb on + strand at 752.213 kb on + strand at 752.214 kb on - strand at 752.254 kb on - strand at 752.274 kb on + strand at 752.274 kb on + strand at 752.275 kb on - strand at 752.275 kb on - strand at 752.714 kb on + strand, within SM_b21111 at 752.714 kb on + strand, within SM_b21111 at 752.715 kb on - strand at 752.718 kb on + strand, within SM_b21111 at 752.718 kb on + strand, within SM_b21111 at 752.805 kb on - strand, within SM_b21111 at 752.805 kb on - strand, within SM_b21111 at 752.889 kb on - strand at 752.889 kb on - strand at 752.907 kb on - strand at 752.907 kb on - strand at 752.925 kb on - strand at 752.992 kb on - strand at 753.064 kb on + strand, within SM_b21112 at 753.213 kb on + strand, within SM_b21112 at 753.213 kb on + strand, within SM_b21112 at 753.384 kb on + strand, within SM_b21112 at 753.385 kb on - strand, within SM_b21112 at 753.423 kb on + strand, within SM_b21112 at 753.423 kb on + strand, within SM_b21112 at 753.628 kb on - strand, within SM_b21112 at 753.651 kb on + strand, within SM_b21112 at 753.651 kb on + strand, within SM_b21112 at 753.687 kb on - strand, within SM_b21112 at 753.776 kb on - strand at 753.824 kb on + strand at 753.824 kb on + strand at 753.825 kb on - strand at 753.932 kb on + strand at 753.932 kb on + strand at 753.933 kb on - strand at 753.933 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 751,256 + SM_b21109 0.53 +0.8 751,256 + SM_b21109 0.53 -1.5 751,256 + SM_b21109 0.53 +0.4 751,257 - SM_b21109 0.54 +0.0 751,472 + SM_b21109 0.73 -0.3 751,473 - SM_b21109 0.73 -0.5 751,473 - SM_b21109 0.73 +0.7 751,473 - SM_b21109 0.73 +0.5 751,779 + +1.1 751,783 - -0.2 751,783 - -1.0 751,783 - +0.1 751,783 - +0.8 751,791 + -1.2 751,791 + -1.5 752,008 - SM_b21110 0.53 +0.1 752,008 - SM_b21110 0.53 -1.6 752,054 + SM_b21110 0.63 +1.8 752,055 - SM_b21110 0.64 +0.2 752,213 + +0.8 752,213 + -1.1 752,214 - +0.1 752,254 - +0.1 752,274 + +0.8 752,274 + -2.9 752,275 - +1.1 752,275 - +1.5 752,714 + SM_b21111 0.67 +0.8 752,714 + SM_b21111 0.67 +4.5 752,715 - -0.2 752,718 + SM_b21111 0.68 -1.2 752,718 + SM_b21111 0.68 +0.1 752,805 - SM_b21111 0.80 +0.7 752,805 - SM_b21111 0.80 +0.8 752,889 - +0.2 752,889 - -0.3 752,907 - -2.0 752,907 - +1.2 752,925 - +1.6 752,992 - +0.0 753,064 + SM_b21112 0.10 +0.1 753,213 + SM_b21112 0.28 -0.9 753,213 + SM_b21112 0.28 -3.2 753,384 + SM_b21112 0.48 -0.1 753,385 - SM_b21112 0.48 -4.2 753,423 + SM_b21112 0.53 +3.2 753,423 + SM_b21112 0.53 -0.0 753,628 - SM_b21112 0.77 -3.2 753,651 + SM_b21112 0.80 -2.2 753,651 + SM_b21112 0.80 -0.8 753,687 - SM_b21112 0.84 +0.2 753,776 - +1.0 753,824 + +0.8 753,824 + +0.3 753,825 - +2.0 753,932 + -2.0 753,932 + +0.8 753,933 - -0.5 753,933 - -0.1
Or see this region's nucleotide sequence