Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21087 and SM_b21088 are separated by 323 nucleotides SM_b21088 and SM_b21089 are separated by 162 nucleotides SM_b21089 and SM_b21090 are separated by 85 nucleotides
SM_b21087: SM_b21087 - conjugal transfer protein, at 725,979 to 730,598
_b21087
SM_b21088: SM_b21088 - hypothetical protein, at 730,922 to 731,137
_b21088
SM_b21089: SM_b21089 - membrane-anchored racemase, at 731,300 to 732,370
_b21089
SM_b21090: SM_b21090 - hypothetical protein, at 732,456 to 733,592
_b21090
Position (kb) 731
732
733 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 730.571 kb on + strand at 730.589 kb on + strand at 730.590 kb on - strand at 730.590 kb on - strand at 730.596 kb on + strand at 730.597 kb on - strand at 730.597 kb on - strand at 730.597 kb on - strand at 730.677 kb on - strand at 730.784 kb on + strand at 730.784 kb on + strand at 730.785 kb on - strand at 730.912 kb on + strand at 730.912 kb on + strand at 730.913 kb on - strand at 730.938 kb on - strand at 730.998 kb on + strand, within SM_b21088 at 730.999 kb on - strand, within SM_b21088 at 730.999 kb on - strand, within SM_b21088 at 730.999 kb on - strand, within SM_b21088 at 730.999 kb on - strand, within SM_b21088 at 731.015 kb on + strand, within SM_b21088 at 731.015 kb on + strand, within SM_b21088 at 731.015 kb on + strand, within SM_b21088 at 731.106 kb on + strand, within SM_b21088 at 731.141 kb on + strand at 731.141 kb on + strand at 731.142 kb on - strand at 731.142 kb on - strand at 731.155 kb on - strand at 731.291 kb on + strand at 731.291 kb on + strand at 731.291 kb on + strand at 731.292 kb on - strand at 731.295 kb on + strand at 731.296 kb on - strand at 731.296 kb on - strand at 731.296 kb on - strand at 731.296 kb on - strand at 731.372 kb on + strand at 731.373 kb on - strand at 731.532 kb on + strand, within SM_b21089 at 731.594 kb on - strand, within SM_b21089 at 731.594 kb on - strand, within SM_b21089 at 731.631 kb on + strand, within SM_b21089 at 731.632 kb on - strand, within SM_b21089 at 731.642 kb on + strand, within SM_b21089 at 731.678 kb on + strand, within SM_b21089 at 731.679 kb on - strand, within SM_b21089 at 731.679 kb on - strand, within SM_b21089 at 731.679 kb on - strand, within SM_b21089 at 731.696 kb on + strand, within SM_b21089 at 731.884 kb on - strand, within SM_b21089 at 731.949 kb on + strand, within SM_b21089 at 731.949 kb on + strand, within SM_b21089 at 731.950 kb on - strand, within SM_b21089 at 731.950 kb on - strand, within SM_b21089 at 732.018 kb on - strand, within SM_b21089 at 732.033 kb on - strand, within SM_b21089 at 732.041 kb on + strand, within SM_b21089 at 732.042 kb on - strand, within SM_b21089 at 732.042 kb on - strand, within SM_b21089 at 732.129 kb on - strand, within SM_b21089 at 732.621 kb on + strand, within SM_b21090 at 732.621 kb on + strand, within SM_b21090 at 732.621 kb on + strand, within SM_b21090 at 732.621 kb on + strand, within SM_b21090 at 732.622 kb on - strand, within SM_b21090 at 732.759 kb on + strand, within SM_b21090 at 732.930 kb on + strand, within SM_b21090 at 732.930 kb on + strand, within SM_b21090 at 733.134 kb on + strand, within SM_b21090 at 733.134 kb on + strand, within SM_b21090 at 733.134 kb on + strand, within SM_b21090 at 733.134 kb on + strand, within SM_b21090 at 733.135 kb on - strand, within SM_b21090 at 733.141 kb on - strand, within SM_b21090 at 733.150 kb on - strand, within SM_b21090 at 733.250 kb on - strand, within SM_b21090
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 730,571 + +0.9 730,589 + +0.3 730,590 - +1.8 730,590 - +0.2 730,596 + -2.7 730,597 - +0.7 730,597 - +0.2 730,597 - +0.8 730,677 - -3.0 730,784 + +0.8 730,784 + +1.7 730,785 - +1.0 730,912 + -2.6 730,912 + -4.1 730,913 - -0.2 730,938 - -1.0 730,998 + SM_b21088 0.35 +0.5 730,999 - SM_b21088 0.36 -0.7 730,999 - SM_b21088 0.36 +1.0 730,999 - SM_b21088 0.36 +0.4 730,999 - SM_b21088 0.36 +0.8 731,015 + SM_b21088 0.43 +2.4 731,015 + SM_b21088 0.43 +1.8 731,015 + SM_b21088 0.43 +0.2 731,106 + SM_b21088 0.85 -0.7 731,141 + +1.1 731,141 + -0.0 731,142 - -1.3 731,142 - +0.8 731,155 - -1.0 731,291 + -2.1 731,291 + -0.2 731,291 + +0.4 731,292 - -0.8 731,295 + -0.6 731,296 - +0.8 731,296 - +1.8 731,296 - -0.1 731,296 - -0.5 731,372 + +0.5 731,373 - -0.6 731,532 + SM_b21089 0.22 -1.0 731,594 - SM_b21089 0.27 +1.3 731,594 - SM_b21089 0.27 -0.4 731,631 + SM_b21089 0.31 -0.1 731,632 - SM_b21089 0.31 -1.2 731,642 + SM_b21089 0.32 +0.6 731,678 + SM_b21089 0.35 +1.3 731,679 - SM_b21089 0.35 -0.5 731,679 - SM_b21089 0.35 +2.1 731,679 - SM_b21089 0.35 -0.2 731,696 + SM_b21089 0.37 -0.8 731,884 - SM_b21089 0.55 +0.2 731,949 + SM_b21089 0.61 +3.2 731,949 + SM_b21089 0.61 -1.6 731,950 - SM_b21089 0.61 -0.3 731,950 - SM_b21089 0.61 +0.7 732,018 - SM_b21089 0.67 +1.2 732,033 - SM_b21089 0.68 -0.2 732,041 + SM_b21089 0.69 -3.1 732,042 - SM_b21089 0.69 +0.7 732,042 - SM_b21089 0.69 -1.7 732,129 - SM_b21089 0.77 -1.2 732,621 + SM_b21090 0.15 +0.1 732,621 + SM_b21090 0.15 +1.2 732,621 + SM_b21090 0.15 +0.4 732,621 + SM_b21090 0.15 +1.8 732,622 - SM_b21090 0.15 -0.5 732,759 + SM_b21090 0.27 +0.3 732,930 + SM_b21090 0.42 +1.1 732,930 + SM_b21090 0.42 -0.4 733,134 + SM_b21090 0.60 +0.3 733,134 + SM_b21090 0.60 -0.5 733,134 + SM_b21090 0.60 +0.2 733,134 + SM_b21090 0.60 +0.1 733,135 - SM_b21090 0.60 +0.4 733,141 - SM_b21090 0.60 -0.5 733,150 - SM_b21090 0.61 -0.3 733,250 - SM_b21090 0.70 -1.7
Or see this region's nucleotide sequence