Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21086 and SM_b21087 are separated by 144 nucleotides SM_b21087 and SM_b21088 are separated by 323 nucleotides SM_b21088 and SM_b21089 are separated by 162 nucleotides
SM_b21086: SM_b21086 - hypothetical protein, at 725,358 to 725,834
_b21086
SM_b21087: SM_b21087 - conjugal transfer protein, at 725,979 to 730,598
_b21087
SM_b21088: SM_b21088 - hypothetical protein, at 730,922 to 731,137
_b21088
SM_b21089: SM_b21089 - membrane-anchored racemase, at 731,300 to 732,370
_b21089
Position (kb) 725
726
727
728
729
730
731 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 725.064 kb on + strand at 725.122 kb on + strand at 725.122 kb on + strand at 725.123 kb on - strand at 725.123 kb on - strand at 725.125 kb on + strand at 725.125 kb on + strand at 725.125 kb on + strand at 725.126 kb on - strand at 725.126 kb on - strand at 725.167 kb on - strand at 725.230 kb on + strand at 725.231 kb on - strand at 725.231 kb on - strand at 725.354 kb on + strand at 725.354 kb on + strand at 725.354 kb on + strand at 725.355 kb on - strand at 725.375 kb on - strand at 725.375 kb on - strand at 725.412 kb on + strand, within SM_b21086 at 725.412 kb on + strand, within SM_b21086 at 725.412 kb on + strand, within SM_b21086 at 725.413 kb on - strand, within SM_b21086 at 725.413 kb on - strand, within SM_b21086 at 725.413 kb on - strand, within SM_b21086 at 725.451 kb on + strand, within SM_b21086 at 725.452 kb on - strand, within SM_b21086 at 725.452 kb on - strand, within SM_b21086 at 725.452 kb on - strand, within SM_b21086 at 725.492 kb on - strand, within SM_b21086 at 725.496 kb on - strand, within SM_b21086 at 725.496 kb on - strand, within SM_b21086 at 725.506 kb on - strand, within SM_b21086 at 725.543 kb on - strand, within SM_b21086 at 725.543 kb on - strand, within SM_b21086 at 725.550 kb on + strand, within SM_b21086 at 725.550 kb on + strand, within SM_b21086 at 725.802 kb on + strand at 725.803 kb on - strand at 725.803 kb on - strand at 725.803 kb on - strand at 725.803 kb on - strand at 725.803 kb on - strand at 725.823 kb on + strand at 725.827 kb on + strand at 725.854 kb on - strand at 725.877 kb on - strand at 725.937 kb on - strand at 725.937 kb on - strand at 726.102 kb on + strand at 726.381 kb on + strand at 726.381 kb on + strand at 726.877 kb on - strand, within SM_b21087 at 728.333 kb on + strand, within SM_b21087 at 728.433 kb on + strand, within SM_b21087 at 728.502 kb on - strand, within SM_b21087 at 730.571 kb on + strand at 730.589 kb on + strand at 730.590 kb on - strand at 730.590 kb on - strand at 730.596 kb on + strand at 730.597 kb on - strand at 730.597 kb on - strand at 730.597 kb on - strand at 730.677 kb on - strand at 730.784 kb on + strand at 730.784 kb on + strand at 730.785 kb on - strand at 730.912 kb on + strand at 730.912 kb on + strand at 730.913 kb on - strand at 730.938 kb on - strand at 730.998 kb on + strand, within SM_b21088 at 730.999 kb on - strand, within SM_b21088 at 730.999 kb on - strand, within SM_b21088 at 730.999 kb on - strand, within SM_b21088 at 730.999 kb on - strand, within SM_b21088 at 731.015 kb on + strand, within SM_b21088 at 731.015 kb on + strand, within SM_b21088 at 731.015 kb on + strand, within SM_b21088 at 731.106 kb on + strand, within SM_b21088 at 731.141 kb on + strand at 731.141 kb on + strand at 731.142 kb on - strand at 731.142 kb on - strand at 731.155 kb on - strand at 731.291 kb on + strand at 731.291 kb on + strand at 731.291 kb on + strand at 731.292 kb on - strand at 731.295 kb on + strand at 731.296 kb on - strand at 731.296 kb on - strand at 731.296 kb on - strand at 731.296 kb on - strand at 731.372 kb on + strand at 731.373 kb on - strand at 731.532 kb on + strand, within SM_b21089 at 731.594 kb on - strand, within SM_b21089 at 731.594 kb on - strand, within SM_b21089
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 725,064 + +0.7 725,122 + -0.3 725,122 + +0.4 725,123 - -0.2 725,123 - -1.2 725,125 + -0.2 725,125 + +0.4 725,125 + -0.7 725,126 - +0.3 725,126 - -1.7 725,167 - -0.0 725,230 + +2.5 725,231 - -3.7 725,231 - -2.9 725,354 + +0.8 725,354 + +0.0 725,354 + -1.8 725,355 - +1.0 725,375 - -0.1 725,375 - -0.1 725,412 + SM_b21086 0.11 +0.9 725,412 + SM_b21086 0.11 +1.4 725,412 + SM_b21086 0.11 -0.1 725,413 - SM_b21086 0.12 +0.6 725,413 - SM_b21086 0.12 -0.7 725,413 - SM_b21086 0.12 -0.0 725,451 + SM_b21086 0.19 -0.3 725,452 - SM_b21086 0.20 +1.0 725,452 - SM_b21086 0.20 -0.8 725,452 - SM_b21086 0.20 -1.5 725,492 - SM_b21086 0.28 +0.3 725,496 - SM_b21086 0.29 -0.5 725,496 - SM_b21086 0.29 -1.2 725,506 - SM_b21086 0.31 -2.8 725,543 - SM_b21086 0.39 -0.3 725,543 - SM_b21086 0.39 +0.4 725,550 + SM_b21086 0.40 +1.1 725,550 + SM_b21086 0.40 +1.3 725,802 + +0.7 725,803 - -0.3 725,803 - +0.1 725,803 - +1.3 725,803 - -1.5 725,803 - -1.4 725,823 + +0.8 725,827 + +0.0 725,854 - -2.0 725,877 - -0.1 725,937 - -1.0 725,937 - -0.3 726,102 + +0.7 726,381 + -2.8 726,381 + -0.1 726,877 - SM_b21087 0.19 -1.5 728,333 + SM_b21087 0.51 -0.3 728,433 + SM_b21087 0.53 -0.4 728,502 - SM_b21087 0.55 -0.3 730,571 + +0.9 730,589 + +0.3 730,590 - +1.8 730,590 - +0.2 730,596 + -2.7 730,597 - +0.7 730,597 - +0.2 730,597 - +0.8 730,677 - -3.0 730,784 + +0.8 730,784 + +1.7 730,785 - +1.0 730,912 + -2.6 730,912 + -4.1 730,913 - -0.2 730,938 - -1.0 730,998 + SM_b21088 0.35 +0.5 730,999 - SM_b21088 0.36 -0.7 730,999 - SM_b21088 0.36 +1.0 730,999 - SM_b21088 0.36 +0.4 730,999 - SM_b21088 0.36 +0.8 731,015 + SM_b21088 0.43 +2.4 731,015 + SM_b21088 0.43 +1.8 731,015 + SM_b21088 0.43 +0.2 731,106 + SM_b21088 0.85 -0.7 731,141 + +1.1 731,141 + -0.0 731,142 - -1.3 731,142 - +0.8 731,155 - -1.0 731,291 + -2.1 731,291 + -0.2 731,291 + +0.4 731,292 - -0.8 731,295 + -0.6 731,296 - +0.8 731,296 - +1.8 731,296 - -0.1 731,296 - -0.5 731,372 + +0.5 731,373 - -0.6 731,532 + SM_b21089 0.22 -1.0 731,594 - SM_b21089 0.27 +1.3 731,594 - SM_b21089 0.27 -0.4
Or see this region's nucleotide sequence