Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21051 and SM_b21052 are separated by 303 nucleotides SM_b21052 and SM_b21053 are separated by 19 nucleotides
SM_b21051: SM_b21051 - udpglucosegdpmannose dehydrogenase, at 683,928 to 685,277
_b21051
SM_b21052: SM_b21052 - epimerase dehydratase, at 685,581 to 686,546
_b21052
SM_b21053: SM_b21053 - membrane-anchored glycosyltransferase, at 686,566 to 687,816
_b21053
Position (kb)
685
686
687 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 684.588 kb on + strand, within SM_b21051 at 684.589 kb on - strand, within SM_b21051 at 684.589 kb on - strand, within SM_b21051 at 684.600 kb on + strand, within SM_b21051 at 684.600 kb on + strand, within SM_b21051 at 684.664 kb on + strand, within SM_b21051 at 684.664 kb on + strand, within SM_b21051 at 684.665 kb on - strand, within SM_b21051 at 684.665 kb on - strand, within SM_b21051 at 684.773 kb on - strand, within SM_b21051 at 684.867 kb on + strand, within SM_b21051 at 684.867 kb on + strand, within SM_b21051 at 684.868 kb on - strand, within SM_b21051 at 685.077 kb on + strand, within SM_b21051 at 685.077 kb on + strand, within SM_b21051 at 685.077 kb on + strand, within SM_b21051 at 685.077 kb on + strand, within SM_b21051 at 685.078 kb on - strand, within SM_b21051 at 685.166 kb on + strand at 685.167 kb on - strand at 685.187 kb on - strand at 685.200 kb on - strand at 685.277 kb on + strand at 685.278 kb on - strand at 685.278 kb on - strand at 685.427 kb on - strand at 685.572 kb on - strand at 685.709 kb on + strand, within SM_b21052 at 685.710 kb on - strand, within SM_b21052 at 685.745 kb on - strand, within SM_b21052 at 685.788 kb on + strand, within SM_b21052 at 685.789 kb on - strand, within SM_b21052 at 685.878 kb on - strand, within SM_b21052 at 685.935 kb on + strand, within SM_b21052 at 686.022 kb on + strand, within SM_b21052 at 686.023 kb on - strand, within SM_b21052 at 686.053 kb on - strand, within SM_b21052 at 686.056 kb on + strand, within SM_b21052 at 686.056 kb on + strand, within SM_b21052 at 686.057 kb on - strand, within SM_b21052 at 686.057 kb on - strand, within SM_b21052 at 686.057 kb on - strand, within SM_b21052 at 686.064 kb on + strand, within SM_b21052 at 686.064 kb on + strand, within SM_b21052 at 686.065 kb on - strand, within SM_b21052 at 686.065 kb on - strand, within SM_b21052 at 686.115 kb on + strand at 686.115 kb on + strand, within SM_b21052 at 686.115 kb on + strand, within SM_b21052 at 686.116 kb on - strand, within SM_b21052 at 686.116 kb on - strand, within SM_b21052 at 686.116 kb on - strand, within SM_b21052 at 686.118 kb on - strand, within SM_b21052 at 686.227 kb on - strand, within SM_b21052 at 686.393 kb on + strand, within SM_b21052 at 686.394 kb on - strand, within SM_b21052 at 686.394 kb on - strand, within SM_b21052 at 686.482 kb on + strand at 686.483 kb on - strand at 686.483 kb on - strand at 686.483 kb on - strand at 686.686 kb on + strand at 686.687 kb on - strand at 686.698 kb on + strand, within SM_b21053 at 686.848 kb on + strand, within SM_b21053 at 686.849 kb on - strand, within SM_b21053 at 686.850 kb on + strand, within SM_b21053 at 686.850 kb on + strand, within SM_b21053 at 686.850 kb on + strand, within SM_b21053 at 686.851 kb on - strand, within SM_b21053 at 686.851 kb on - strand, within SM_b21053 at 686.851 kb on - strand, within SM_b21053 at 686.851 kb on - strand, within SM_b21053 at 686.870 kb on - strand, within SM_b21053 at 686.912 kb on - strand, within SM_b21053 at 686.912 kb on - strand, within SM_b21053 at 686.934 kb on - strand, within SM_b21053 at 686.956 kb on - strand, within SM_b21053 at 687.062 kb on + strand, within SM_b21053 at 687.062 kb on + strand, within SM_b21053 at 687.062 kb on + strand, within SM_b21053 at 687.191 kb on + strand, within SM_b21053 at 687.212 kb on + strand, within SM_b21053 at 687.242 kb on + strand, within SM_b21053 at 687.242 kb on + strand, within SM_b21053 at 687.304 kb on + strand, within SM_b21053 at 687.377 kb on - strand, within SM_b21053 at 687.401 kb on - strand, within SM_b21053 at 687.496 kb on + strand, within SM_b21053 at 687.497 kb on - strand, within SM_b21053 at 687.497 kb on - strand, within SM_b21053 at 687.514 kb on - strand, within SM_b21053
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 684,588 + SM_b21051 0.49 -0.2 684,589 - SM_b21051 0.49 -3.6 684,589 - SM_b21051 0.49 -1.2 684,600 + SM_b21051 0.50 +2.0 684,600 + SM_b21051 0.50 -0.8 684,664 + SM_b21051 0.55 +1.0 684,664 + SM_b21051 0.55 -2.3 684,665 - SM_b21051 0.55 -1.2 684,665 - SM_b21051 0.55 -1.2 684,773 - SM_b21051 0.63 -2.7 684,867 + SM_b21051 0.70 -0.1 684,867 + SM_b21051 0.70 -0.7 684,868 - SM_b21051 0.70 +2.6 685,077 + SM_b21051 0.85 +4.3 685,077 + SM_b21051 0.85 +4.6 685,077 + SM_b21051 0.85 -2.1 685,077 + SM_b21051 0.85 -0.2 685,078 - SM_b21051 0.85 -2.1 685,166 + +1.9 685,167 - +1.4 685,187 - -0.9 685,200 - -0.1 685,277 + +2.0 685,278 - +0.1 685,278 - +1.0 685,427 - +3.7 685,572 - -0.2 685,709 + SM_b21052 0.13 -3.0 685,710 - SM_b21052 0.13 -2.0 685,745 - SM_b21052 0.17 -0.7 685,788 + SM_b21052 0.21 -1.4 685,789 - SM_b21052 0.22 +0.2 685,878 - SM_b21052 0.31 -0.3 685,935 + SM_b21052 0.37 -0.4 686,022 + SM_b21052 0.46 -1.7 686,023 - SM_b21052 0.46 +1.1 686,053 - SM_b21052 0.49 -0.1 686,056 + SM_b21052 0.49 -1.1 686,056 + SM_b21052 0.49 -0.1 686,057 - SM_b21052 0.49 -3.8 686,057 - SM_b21052 0.49 -0.3 686,057 - SM_b21052 0.49 -0.3 686,064 + SM_b21052 0.50 +0.1 686,064 + SM_b21052 0.50 +0.4 686,065 - SM_b21052 0.50 -1.1 686,065 - SM_b21052 0.50 -2.8 686,115 + -1.1 686,115 + SM_b21052 0.55 -1.7 686,115 + SM_b21052 0.55 +1.3 686,116 - SM_b21052 0.55 +0.6 686,116 - SM_b21052 0.55 +0.9 686,116 - SM_b21052 0.55 +0.9 686,118 - SM_b21052 0.56 -0.0 686,227 - SM_b21052 0.67 -0.7 686,393 + SM_b21052 0.84 +3.8 686,394 - SM_b21052 0.84 -0.6 686,394 - SM_b21052 0.84 +0.9 686,482 + -1.9 686,483 - -0.4 686,483 - +0.4 686,483 - -1.2 686,686 + +0.1 686,687 - -2.8 686,698 + SM_b21053 0.11 -0.5 686,848 + SM_b21053 0.23 -1.1 686,849 - SM_b21053 0.23 +0.5 686,850 + SM_b21053 0.23 +0.2 686,850 + SM_b21053 0.23 -1.5 686,850 + SM_b21053 0.23 +0.9 686,851 - SM_b21053 0.23 -0.8 686,851 - SM_b21053 0.23 -0.0 686,851 - SM_b21053 0.23 -1.0 686,851 - SM_b21053 0.23 +1.9 686,870 - SM_b21053 0.24 +1.1 686,912 - SM_b21053 0.28 -0.1 686,912 - SM_b21053 0.28 -1.6 686,934 - SM_b21053 0.29 +0.9 686,956 - SM_b21053 0.31 +4.4 687,062 + SM_b21053 0.40 -1.9 687,062 + SM_b21053 0.40 -0.9 687,062 + SM_b21053 0.40 -0.3 687,191 + SM_b21053 0.50 -0.6 687,212 + SM_b21053 0.52 +1.0 687,242 + SM_b21053 0.54 +1.4 687,242 + SM_b21053 0.54 +0.7 687,304 + SM_b21053 0.59 +2.6 687,377 - SM_b21053 0.65 +0.3 687,401 - SM_b21053 0.67 +0.1 687,496 + SM_b21053 0.74 -0.6 687,497 - SM_b21053 0.74 +0.2 687,497 - SM_b21053 0.74 +1.9 687,514 - SM_b21053 0.76 -1.1
Or see this region's nucleotide sequence