Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21661 and SM_b21711 overlap by 28 nucleotides SM_b21711 and SM_b21045 are separated by 511 nucleotides SM_b21045 and SM_b21046 are separated by 95 nucleotides
SM_b21661: SM_b21661 - transposase of insertion sequence ISRm10-1, orfA protein, at 674,574 to 674,957
_b21661
SM_b21711: SM_b21711 - transposase of insertion sequence ISRm10-1, orfB N-terminus protein, at 674,930 to 675,061
_b21711
SM_b21045: SM_b21045 - reverse transcriptasematurase, at 675,573 to 676,832
_b21045
SM_b21046: SM_b21046 - transposase of insertion sequence ISRm10-1, orfB C-terminus protein, at 676,928 to 677,401
_b21046
Position (kb) 675
676
677 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 677.090 kb on + strand, within SM_b21046 at 677.137 kb on - strand, within SM_b21046 at 677.209 kb on + strand, within SM_b21046 at 677.209 kb on + strand, within SM_b21046 at 677.209 kb on + strand, within SM_b21046 at 677.209 kb on + strand, within SM_b21046 at 677.210 kb on - strand, within SM_b21046 at 677.228 kb on - strand, within SM_b21046 at 677.369 kb on + strand at 677.369 kb on + strand at 677.369 kb on + strand at 677.369 kb on + strand at 677.387 kb on + strand at 677.415 kb on + strand at 677.416 kb on - strand at 677.416 kb on - strand at 677.416 kb on - strand at 677.516 kb on + strand at 677.516 kb on + strand at 677.526 kb on + strand at 677.566 kb on + strand at 677.573 kb on + strand at 677.596 kb on + strand at 677.596 kb on + strand at 677.597 kb on - strand at 677.597 kb on - strand at 677.597 kb on - strand at 677.721 kb on + strand at 677.722 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 677,090 + SM_b21046 0.34 +2.7 677,137 - SM_b21046 0.44 +0.4 677,209 + SM_b21046 0.59 +1.2 677,209 + SM_b21046 0.59 -3.0 677,209 + SM_b21046 0.59 +0.2 677,209 + SM_b21046 0.59 +1.6 677,210 - SM_b21046 0.59 -0.5 677,228 - SM_b21046 0.63 +0.1 677,369 + +0.2 677,369 + -3.1 677,369 + +2.4 677,369 + +0.7 677,387 + +0.1 677,415 + -0.3 677,416 - +0.1 677,416 - +1.1 677,416 - -0.3 677,516 + -0.8 677,516 + +1.2 677,526 + -1.5 677,566 + +0.9 677,573 + -0.4 677,596 + -0.3 677,596 + -1.8 677,597 - +0.9 677,597 - +0.4 677,597 - +0.3 677,721 + -2.3 677,722 - +0.2
Or see this region's nucleotide sequence