Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21022 and SM_b21023 are separated by 3 nucleotides SM_b21023 and SM_b21024 are separated by 168 nucleotides SM_b21024 and SM_b21025 are separated by 11 nucleotides SM_b21025 and SM_b21026 are separated by 143 nucleotides
SM_b21022: SM_b21022 - autoinducer-2 (AI-2) kinase, at 645,127 to 646,725
_b21022
SM_b21023: SM_b21023 - hypothetical protein, at 646,729 to 647,043
_b21023
SM_b21024: SM_b21024 - hypothetical protein, at 647,212 to 647,379
_b21024
SM_b21025: SM_b21025 - hypothetical protein, at 647,391 to 647,810
_b21025
SM_b21026: SM_b21026 - exopeptide, at 647,954 to 648,091
_b21026
Position (kb) 647
648 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 646.341 kb on - strand, within SM_b21022 at 646.341 kb on - strand, within SM_b21022 at 646.359 kb on + strand, within SM_b21022 at 646.359 kb on + strand, within SM_b21022 at 646.587 kb on + strand at 646.587 kb on + strand at 646.587 kb on + strand at 646.588 kb on - strand at 646.620 kb on + strand at 646.621 kb on - strand at 646.693 kb on + strand at 646.696 kb on - strand at 646.713 kb on + strand at 646.714 kb on - strand at 646.714 kb on - strand at 646.723 kb on + strand at 646.723 kb on + strand at 646.724 kb on - strand at 646.746 kb on + strand at 646.746 kb on + strand at 646.747 kb on - strand at 646.747 kb on - strand at 646.804 kb on + strand, within SM_b21023 at 646.805 kb on - strand, within SM_b21023 at 646.805 kb on - strand, within SM_b21023 at 646.805 kb on - strand, within SM_b21023 at 646.885 kb on + strand, within SM_b21023 at 646.886 kb on - strand, within SM_b21023 at 646.886 kb on - strand, within SM_b21023 at 646.998 kb on - strand, within SM_b21023 at 646.999 kb on + strand, within SM_b21023 at 646.999 kb on + strand, within SM_b21023 at 646.999 kb on + strand, within SM_b21023 at 647.000 kb on - strand, within SM_b21023 at 647.000 kb on - strand, within SM_b21023 at 647.001 kb on + strand, within SM_b21023 at 647.002 kb on - strand, within SM_b21023 at 647.042 kb on - strand at 647.055 kb on + strand at 647.194 kb on - strand at 647.194 kb on - strand at 647.288 kb on + strand, within SM_b21024 at 647.289 kb on - strand, within SM_b21024 at 647.289 kb on - strand, within SM_b21024 at 647.443 kb on - strand, within SM_b21025 at 647.578 kb on + strand, within SM_b21025 at 647.579 kb on - strand, within SM_b21025 at 647.580 kb on + strand, within SM_b21025 at 647.580 kb on + strand, within SM_b21025 at 647.580 kb on + strand, within SM_b21025 at 647.580 kb on + strand, within SM_b21025 at 647.581 kb on - strand, within SM_b21025 at 647.581 kb on - strand, within SM_b21025 at 647.581 kb on - strand, within SM_b21025 at 647.706 kb on - strand, within SM_b21025 at 647.752 kb on - strand, within SM_b21025 at 647.820 kb on - strand at 647.934 kb on + strand at 647.935 kb on - strand at 647.935 kb on - strand at 647.935 kb on - strand at 647.960 kb on + strand at 647.961 kb on - strand at 648.062 kb on + strand, within SM_b21026 at 648.062 kb on + strand, within SM_b21026 at 648.062 kb on + strand, within SM_b21026 at 648.063 kb on - strand, within SM_b21026 at 648.096 kb on + strand at 648.097 kb on - strand at 648.097 kb on - strand at 648.097 kb on - strand at 648.099 kb on + strand at 648.100 kb on - strand at 648.135 kb on + strand at 648.135 kb on + strand at 648.136 kb on - strand at 648.214 kb on + strand at 648.223 kb on + strand at 648.223 kb on + strand at 648.224 kb on - strand at 648.285 kb on - strand at 648.296 kb on + strand at 648.297 kb on - strand at 648.297 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 646,341 - SM_b21022 0.76 +0.7 646,341 - SM_b21022 0.76 -4.2 646,359 + SM_b21022 0.77 +0.4 646,359 + SM_b21022 0.77 +0.4 646,587 + +0.3 646,587 + -1.1 646,587 + +2.9 646,588 - -0.9 646,620 + +0.3 646,621 - -0.4 646,693 + +0.9 646,696 - -0.1 646,713 + +0.9 646,714 - -1.7 646,714 - +0.4 646,723 + -1.2 646,723 + +0.8 646,724 - -2.5 646,746 + +0.3 646,746 + +1.6 646,747 - -0.9 646,747 - -0.3 646,804 + SM_b21023 0.24 -2.0 646,805 - SM_b21023 0.24 +3.9 646,805 - SM_b21023 0.24 -0.3 646,805 - SM_b21023 0.24 +0.6 646,885 + SM_b21023 0.50 -1.0 646,886 - SM_b21023 0.50 -3.4 646,886 - SM_b21023 0.50 -3.6 646,998 - SM_b21023 0.85 -0.9 646,999 + SM_b21023 0.86 -1.7 646,999 + SM_b21023 0.86 +0.3 646,999 + SM_b21023 0.86 -0.9 647,000 - SM_b21023 0.86 +1.7 647,000 - SM_b21023 0.86 -1.0 647,001 + SM_b21023 0.86 -1.0 647,002 - SM_b21023 0.87 -0.6 647,042 - -1.0 647,055 + +0.0 647,194 - +0.7 647,194 - -1.4 647,288 + SM_b21024 0.45 +3.9 647,289 - SM_b21024 0.46 +0.4 647,289 - SM_b21024 0.46 +0.9 647,443 - SM_b21025 0.12 +0.7 647,578 + SM_b21025 0.45 -2.6 647,579 - SM_b21025 0.45 -0.1 647,580 + SM_b21025 0.45 +0.3 647,580 + SM_b21025 0.45 -1.7 647,580 + SM_b21025 0.45 -3.3 647,580 + SM_b21025 0.45 -1.3 647,581 - SM_b21025 0.45 +0.9 647,581 - SM_b21025 0.45 +0.4 647,581 - SM_b21025 0.45 +1.1 647,706 - SM_b21025 0.75 +0.6 647,752 - SM_b21025 0.86 -0.7 647,820 - -0.3 647,934 + +0.2 647,935 - -2.7 647,935 - -1.0 647,935 - -0.1 647,960 + -1.4 647,961 - +3.0 648,062 + SM_b21026 0.78 +0.1 648,062 + SM_b21026 0.78 -0.3 648,062 + SM_b21026 0.78 -0.1 648,063 - SM_b21026 0.79 +0.9 648,096 + -2.7 648,097 - -2.1 648,097 - -1.7 648,097 - -1.2 648,099 + +0.9 648,100 - -0.3 648,135 + -0.5 648,135 + -1.0 648,136 - +1.6 648,214 + -0.0 648,223 + -0.0 648,223 + -0.3 648,224 - -1.6 648,285 - +0.7 648,296 + -0.0 648,297 - +0.1 648,297 - +0.6
Or see this region's nucleotide sequence