Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20999 and SM_b21000 are separated by 152 nucleotides SM_b21000 and SM_b21001 overlap by 14 nucleotides SM_b21001 and SM_b21002 are separated by 77 nucleotides
SM_b20999: SM_b20999 - transporter, at 1,235,141 to 1,236,403
_b20999
SM_b21000: SM_b21000 - transporter, at 1,236,556 to 1,237,689
_b21000
SM_b21001: SM_b21001 - hypothetical protein, at 1,237,676 to 1,238,050
_b21001
SM_b21002: SM_b21002 - methionine aminopeptidase, at 1,238,128 to 1,238,877
_b21002
Position (kb)
1236
1237
1238 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1236.509 kb on - strand at 1236.543 kb on + strand at 1236.653 kb on + strand at 1236.653 kb on + strand at 1236.788 kb on + strand, within SM_b21000 at 1236.789 kb on - strand, within SM_b21000 at 1236.908 kb on + strand, within SM_b21000 at 1236.908 kb on + strand, within SM_b21000 at 1236.953 kb on + strand, within SM_b21000 at 1236.953 kb on + strand, within SM_b21000 at 1236.954 kb on - strand, within SM_b21000 at 1236.954 kb on - strand, within SM_b21000 at 1237.064 kb on + strand, within SM_b21000 at 1237.065 kb on - strand, within SM_b21000 at 1237.085 kb on + strand, within SM_b21000 at 1237.086 kb on - strand, within SM_b21000 at 1237.086 kb on - strand, within SM_b21000 at 1237.288 kb on - strand, within SM_b21000 at 1237.458 kb on + strand at 1237.458 kb on + strand, within SM_b21000 at 1237.458 kb on + strand, within SM_b21000 at 1237.458 kb on + strand, within SM_b21000 at 1237.458 kb on + strand, within SM_b21000 at 1237.458 kb on + strand, within SM_b21000 at 1237.458 kb on + strand, within SM_b21000 at 1237.458 kb on + strand, within SM_b21000 at 1237.459 kb on - strand, within SM_b21000 at 1237.459 kb on - strand, within SM_b21000 at 1237.459 kb on - strand, within SM_b21000 at 1237.459 kb on - strand, within SM_b21000 at 1237.569 kb on - strand, within SM_b21000 at 1237.654 kb on - strand at 1237.655 kb on + strand at 1237.655 kb on + strand at 1237.656 kb on - strand at 1237.866 kb on + strand, within SM_b21001 at 1237.866 kb on + strand, within SM_b21001 at 1237.866 kb on + strand, within SM_b21001 at 1237.867 kb on - strand, within SM_b21001 at 1238.187 kb on - strand at 1238.187 kb on - strand at 1238.216 kb on + strand, within SM_b21002 at 1238.216 kb on + strand, within SM_b21002 at 1238.604 kb on + strand, within SM_b21002 at 1238.604 kb on + strand, within SM_b21002 at 1238.605 kb on - strand, within SM_b21002
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,236,509 - -2.7 1,236,543 + -1.1 1,236,653 + -0.1 1,236,653 + -0.8 1,236,788 + SM_b21000 0.20 -0.4 1,236,789 - SM_b21000 0.21 -0.1 1,236,908 + SM_b21000 0.31 +1.6 1,236,908 + SM_b21000 0.31 -0.5 1,236,953 + SM_b21000 0.35 -0.7 1,236,953 + SM_b21000 0.35 -0.8 1,236,954 - SM_b21000 0.35 +1.5 1,236,954 - SM_b21000 0.35 +0.5 1,237,064 + SM_b21000 0.45 -0.0 1,237,065 - SM_b21000 0.45 -1.1 1,237,085 + SM_b21000 0.47 +0.2 1,237,086 - SM_b21000 0.47 -1.1 1,237,086 - SM_b21000 0.47 +0.9 1,237,288 - SM_b21000 0.65 +0.0 1,237,458 + +0.9 1,237,458 + SM_b21000 0.80 -2.6 1,237,458 + SM_b21000 0.80 -0.1 1,237,458 + SM_b21000 0.80 -3.3 1,237,458 + SM_b21000 0.80 -0.1 1,237,458 + SM_b21000 0.80 -0.3 1,237,458 + SM_b21000 0.80 -0.2 1,237,458 + SM_b21000 0.80 -0.0 1,237,459 - SM_b21000 0.80 -3.0 1,237,459 - SM_b21000 0.80 -0.1 1,237,459 - SM_b21000 0.80 -1.4 1,237,459 - SM_b21000 0.80 -0.9 1,237,569 - SM_b21000 0.89 -0.7 1,237,654 - -0.1 1,237,655 + -0.1 1,237,655 + -0.5 1,237,656 - +0.1 1,237,866 + SM_b21001 0.51 +0.3 1,237,866 + SM_b21001 0.51 -1.3 1,237,866 + SM_b21001 0.51 -1.1 1,237,867 - SM_b21001 0.51 +0.3 1,238,187 - -2.2 1,238,187 - +0.9 1,238,216 + SM_b21002 0.12 -1.0 1,238,216 + SM_b21002 0.12 +2.5 1,238,604 + SM_b21002 0.63 -2.1 1,238,604 + SM_b21002 0.63 +0.9 1,238,605 - SM_b21002 0.64 +0.5
Or see this region's nucleotide sequence