Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20926 and SM_b20927 are separated by 8 nucleotides SM_b20927 and SM_b20928 are separated by 174 nucleotides SM_b20928 and SM_b20929 are separated by 54 nucleotides
SM_b20926: SM_b20926 - hypothetical protein, at 1,150,422 to 1,150,922
_b20926
SM_b20927: SM_b20927 - hypothetical protein, at 1,150,931 to 1,151,413
_b20927
SM_b20928: SM_b20928 - carboxyl-terminal protease, at 1,151,588 to 1,152,838
_b20928
SM_b20929: SM_b20929 - sugar uptake ABC transporter permease, at 1,152,893 to 1,153,891
_b20929
Position (kb)
1151
1152
1153 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1150.886 kb on - strand at 1150.886 kb on - strand at 1150.895 kb on - strand at 1150.912 kb on + strand at 1150.912 kb on + strand at 1150.912 kb on + strand at 1150.912 kb on + strand at 1150.913 kb on - strand at 1150.932 kb on + strand at 1150.932 kb on + strand at 1151.032 kb on + strand, within SM_b20927 at 1151.032 kb on + strand, within SM_b20927 at 1151.033 kb on - strand, within SM_b20927 at 1151.197 kb on - strand, within SM_b20927 at 1151.220 kb on - strand, within SM_b20927 at 1151.220 kb on - strand, within SM_b20927 at 1151.339 kb on + strand, within SM_b20927 at 1151.340 kb on - strand, within SM_b20927 at 1151.340 kb on - strand, within SM_b20927 at 1151.403 kb on + strand at 1151.403 kb on + strand at 1151.424 kb on + strand at 1151.424 kb on + strand at 1151.424 kb on + strand at 1151.425 kb on - strand at 1151.425 kb on - strand at 1151.425 kb on - strand at 1151.425 kb on - strand at 1151.425 kb on - strand at 1151.425 kb on - strand at 1151.425 kb on - strand at 1151.450 kb on - strand at 1151.487 kb on - strand at 1151.491 kb on + strand at 1151.491 kb on + strand at 1151.491 kb on + strand at 1151.491 kb on + strand at 1151.491 kb on + strand at 1151.492 kb on - strand at 1151.492 kb on - strand at 1151.550 kb on - strand at 1151.550 kb on - strand at 1151.559 kb on + strand at 1151.559 kb on + strand at 1151.897 kb on + strand, within SM_b20928 at 1151.897 kb on + strand, within SM_b20928 at 1151.900 kb on + strand, within SM_b20928 at 1151.900 kb on + strand, within SM_b20928 at 1151.901 kb on - strand, within SM_b20928 at 1151.901 kb on - strand, within SM_b20928 at 1151.979 kb on - strand, within SM_b20928 at 1151.979 kb on - strand, within SM_b20928 at 1151.979 kb on - strand, within SM_b20928 at 1152.038 kb on + strand, within SM_b20928 at 1152.039 kb on - strand, within SM_b20928 at 1152.194 kb on - strand, within SM_b20928 at 1152.194 kb on - strand, within SM_b20928 at 1152.493 kb on + strand, within SM_b20928 at 1152.494 kb on - strand, within SM_b20928 at 1152.494 kb on - strand, within SM_b20928 at 1152.494 kb on - strand, within SM_b20928 at 1152.494 kb on - strand, within SM_b20928 at 1152.494 kb on - strand, within SM_b20928 at 1152.537 kb on + strand, within SM_b20928 at 1152.538 kb on - strand, within SM_b20928 at 1152.538 kb on - strand, within SM_b20928 at 1152.538 kb on - strand, within SM_b20928 at 1152.647 kb on + strand, within SM_b20928 at 1152.648 kb on - strand, within SM_b20928 at 1152.697 kb on - strand, within SM_b20928 at 1152.744 kb on + strand at 1152.744 kb on + strand at 1152.858 kb on + strand at 1152.859 kb on - strand at 1152.859 kb on - strand at 1153.203 kb on + strand, within SM_b20929 at 1153.203 kb on + strand, within SM_b20929 at 1153.203 kb on + strand, within SM_b20929 at 1153.203 kb on + strand, within SM_b20929 at 1153.203 kb on + strand, within SM_b20929 at 1153.204 kb on - strand, within SM_b20929 at 1153.204 kb on - strand, within SM_b20929 at 1153.205 kb on + strand, within SM_b20929 at 1153.205 kb on + strand, within SM_b20929 at 1153.341 kb on + strand, within SM_b20929 at 1153.342 kb on - strand, within SM_b20929 at 1153.342 kb on - strand, within SM_b20929 at 1153.425 kb on - strand, within SM_b20929 at 1153.425 kb on - strand, within SM_b20929 at 1153.425 kb on - strand, within SM_b20929 at 1153.432 kb on + strand, within SM_b20929 at 1153.433 kb on - strand, within SM_b20929 at 1153.509 kb on - strand, within SM_b20929 at 1153.512 kb on + strand, within SM_b20929 at 1153.512 kb on + strand, within SM_b20929 at 1153.512 kb on + strand, within SM_b20929 at 1153.513 kb on - strand, within SM_b20929 at 1153.573 kb on + strand, within SM_b20929 at 1153.669 kb on + strand, within SM_b20929 at 1153.734 kb on + strand, within SM_b20929 at 1153.735 kb on - strand, within SM_b20929
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,150,886 - -2.5 1,150,886 - -0.7 1,150,895 - +0.8 1,150,912 + -0.5 1,150,912 + -0.3 1,150,912 + -0.7 1,150,912 + -2.0 1,150,913 - -0.0 1,150,932 + +0.1 1,150,932 + +0.7 1,151,032 + SM_b20927 0.21 +0.5 1,151,032 + SM_b20927 0.21 -1.0 1,151,033 - SM_b20927 0.21 +0.2 1,151,197 - SM_b20927 0.55 -0.7 1,151,220 - SM_b20927 0.60 +0.4 1,151,220 - SM_b20927 0.60 -3.0 1,151,339 + SM_b20927 0.84 -1.4 1,151,340 - SM_b20927 0.85 -0.0 1,151,340 - SM_b20927 0.85 -0.9 1,151,403 + -3.3 1,151,403 + -0.4 1,151,424 + -0.8 1,151,424 + -0.1 1,151,424 + -1.1 1,151,425 - -1.2 1,151,425 - -0.7 1,151,425 - -1.1 1,151,425 - -2.5 1,151,425 - -1.7 1,151,425 - +0.9 1,151,425 - +1.4 1,151,450 - -1.1 1,151,487 - +1.6 1,151,491 + -2.1 1,151,491 + +1.3 1,151,491 + -1.4 1,151,491 + -0.2 1,151,491 + +0.2 1,151,492 - -0.3 1,151,492 - -2.8 1,151,550 - -0.7 1,151,550 - -1.1 1,151,559 + -0.8 1,151,559 + -2.2 1,151,897 + SM_b20928 0.25 +1.7 1,151,897 + SM_b20928 0.25 +0.6 1,151,900 + SM_b20928 0.25 +1.4 1,151,900 + SM_b20928 0.25 +0.2 1,151,901 - SM_b20928 0.25 -0.3 1,151,901 - SM_b20928 0.25 -1.6 1,151,979 - SM_b20928 0.31 +1.2 1,151,979 - SM_b20928 0.31 -0.7 1,151,979 - SM_b20928 0.31 -1.0 1,152,038 + SM_b20928 0.36 -3.3 1,152,039 - SM_b20928 0.36 +1.1 1,152,194 - SM_b20928 0.48 +1.7 1,152,194 - SM_b20928 0.48 +0.3 1,152,493 + SM_b20928 0.72 +0.8 1,152,494 - SM_b20928 0.72 +0.8 1,152,494 - SM_b20928 0.72 +0.5 1,152,494 - SM_b20928 0.72 -0.4 1,152,494 - SM_b20928 0.72 -0.3 1,152,494 - SM_b20928 0.72 -0.3 1,152,537 + SM_b20928 0.76 +1.9 1,152,538 - SM_b20928 0.76 -0.0 1,152,538 - SM_b20928 0.76 -3.6 1,152,538 - SM_b20928 0.76 -2.0 1,152,647 + SM_b20928 0.85 -1.2 1,152,648 - SM_b20928 0.85 +0.5 1,152,697 - SM_b20928 0.89 -1.9 1,152,744 + -2.7 1,152,744 + +2.8 1,152,858 + -0.4 1,152,859 - +0.1 1,152,859 - +1.3 1,153,203 + SM_b20929 0.31 +0.3 1,153,203 + SM_b20929 0.31 -0.9 1,153,203 + SM_b20929 0.31 -3.3 1,153,203 + SM_b20929 0.31 +0.4 1,153,203 + SM_b20929 0.31 -0.4 1,153,204 - SM_b20929 0.31 -2.9 1,153,204 - SM_b20929 0.31 -1.6 1,153,205 + SM_b20929 0.31 -0.1 1,153,205 + SM_b20929 0.31 -0.0 1,153,341 + SM_b20929 0.45 -0.4 1,153,342 - SM_b20929 0.45 +1.8 1,153,342 - SM_b20929 0.45 -0.3 1,153,425 - SM_b20929 0.53 -0.4 1,153,425 - SM_b20929 0.53 -1.9 1,153,425 - SM_b20929 0.53 -0.4 1,153,432 + SM_b20929 0.54 +0.5 1,153,433 - SM_b20929 0.54 +0.4 1,153,509 - SM_b20929 0.62 -2.6 1,153,512 + SM_b20929 0.62 +1.4 1,153,512 + SM_b20929 0.62 +2.1 1,153,512 + SM_b20929 0.62 -1.1 1,153,513 - SM_b20929 0.62 -0.7 1,153,573 + SM_b20929 0.68 +1.7 1,153,669 + SM_b20929 0.78 -1.1 1,153,734 + SM_b20929 0.84 +0.1 1,153,735 - SM_b20929 0.84 -0.3
Or see this region's nucleotide sequence