Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20897 and SM_b20898 are separated by 3 nucleotides SM_b20898 and SM_b21678 are separated by 52 nucleotides SM_b21678 and SM_b20899 are separated by 250 nucleotides SM_b20899 and SM_b20900 are separated by 63 nucleotides
SM_b20897: SM_b20897 - hypothetical protein, at 1,305,414 to 1,305,866
_b20897
SM_b20898: SM_b20898 - hypothetical protein, at 1,305,870 to 1,306,280
_b20898
SM_b21678: SM_b21678 - hypothetical protein, at 1,306,333 to 1,306,524
_b21678
SM_b20899: SM_b20899 - Inositol 2-dehydrogenase (EC 1.1.1.18) (from data) , at 1,306,775 to 1,307,767
_b20899
SM_b20900: SM_b20900 - hypothetical protein, at 1,307,831 to 1,309,765
_b20900
Position (kb)
1306
1307
1308 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1305.865 kb on - strand at 1305.865 kb on - strand at 1305.964 kb on + strand, within SM_b20898 at 1306.032 kb on + strand, within SM_b20898 at 1306.069 kb on - strand, within SM_b20898 at 1306.078 kb on + strand, within SM_b20898 at 1306.079 kb on - strand, within SM_b20898 at 1306.079 kb on - strand, within SM_b20898 at 1306.079 kb on - strand, within SM_b20898 at 1306.134 kb on + strand, within SM_b20898 at 1306.135 kb on - strand, within SM_b20898 at 1306.135 kb on - strand, within SM_b20898 at 1306.149 kb on + strand, within SM_b20898 at 1306.150 kb on - strand, within SM_b20898 at 1306.150 kb on - strand, within SM_b20898 at 1306.150 kb on - strand, within SM_b20898 at 1306.157 kb on - strand, within SM_b20898 at 1306.168 kb on + strand, within SM_b20898 at 1306.168 kb on + strand, within SM_b20898 at 1306.172 kb on + strand, within SM_b20898 at 1306.172 kb on + strand, within SM_b20898 at 1306.180 kb on - strand, within SM_b20898 at 1306.180 kb on - strand, within SM_b20898 at 1306.180 kb on - strand, within SM_b20898 at 1306.180 kb on - strand, within SM_b20898 at 1306.243 kb on + strand at 1306.243 kb on + strand at 1306.243 kb on + strand at 1306.243 kb on + strand at 1306.247 kb on + strand at 1306.247 kb on + strand at 1306.247 kb on + strand at 1306.248 kb on - strand at 1306.363 kb on + strand, within SM_b21678 at 1306.364 kb on - strand, within SM_b21678 at 1306.586 kb on + strand at 1306.586 kb on + strand at 1306.594 kb on - strand at 1306.993 kb on + strand, within SM_b20899 at 1307.253 kb on - strand, within SM_b20899 at 1307.397 kb on - strand, within SM_b20899 at 1307.471 kb on + strand, within SM_b20899 at 1307.471 kb on + strand, within SM_b20899 at 1307.471 kb on + strand, within SM_b20899 at 1307.471 kb on + strand, within SM_b20899 at 1307.472 kb on - strand, within SM_b20899 at 1307.570 kb on + strand, within SM_b20899 at 1307.570 kb on + strand, within SM_b20899 at 1307.570 kb on + strand, within SM_b20899 at 1307.571 kb on - strand, within SM_b20899 at 1307.571 kb on - strand, within SM_b20899 at 1307.609 kb on + strand, within SM_b20899 at 1307.611 kb on + strand, within SM_b20899 at 1307.622 kb on - strand, within SM_b20899 at 1307.647 kb on - strand, within SM_b20899 at 1307.832 kb on + strand at 1307.832 kb on + strand at 1307.832 kb on + strand at 1307.832 kb on + strand at 1307.832 kb on + strand at 1307.833 kb on - strand at 1307.833 kb on - strand at 1308.033 kb on - strand, within SM_b20900 at 1308.033 kb on - strand, within SM_b20900 at 1308.280 kb on - strand, within SM_b20900 at 1308.321 kb on + strand, within SM_b20900 at 1308.453 kb on + strand, within SM_b20900 at 1308.560 kb on - strand, within SM_b20900
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,305,865 - -0.5 1,305,865 - +0.6 1,305,964 + SM_b20898 0.23 -2.8 1,306,032 + SM_b20898 0.39 +1.4 1,306,069 - SM_b20898 0.48 -1.2 1,306,078 + SM_b20898 0.51 +0.6 1,306,079 - SM_b20898 0.51 -0.0 1,306,079 - SM_b20898 0.51 +0.1 1,306,079 - SM_b20898 0.51 +0.5 1,306,134 + SM_b20898 0.64 -1.4 1,306,135 - SM_b20898 0.64 -2.1 1,306,135 - SM_b20898 0.64 -0.2 1,306,149 + SM_b20898 0.68 +0.9 1,306,150 - SM_b20898 0.68 +0.1 1,306,150 - SM_b20898 0.68 +0.8 1,306,150 - SM_b20898 0.68 -0.5 1,306,157 - SM_b20898 0.70 -3.1 1,306,168 + SM_b20898 0.73 +0.8 1,306,168 + SM_b20898 0.73 -1.5 1,306,172 + SM_b20898 0.73 +1.8 1,306,172 + SM_b20898 0.73 -1.6 1,306,180 - SM_b20898 0.75 +1.9 1,306,180 - SM_b20898 0.75 -0.7 1,306,180 - SM_b20898 0.75 -4.2 1,306,180 - SM_b20898 0.75 +1.3 1,306,243 + +3.9 1,306,243 + -0.2 1,306,243 + -0.5 1,306,243 + +0.0 1,306,247 + +2.4 1,306,247 + -0.5 1,306,247 + +0.7 1,306,248 - +0.4 1,306,363 + SM_b21678 0.16 +1.0 1,306,364 - SM_b21678 0.16 +1.7 1,306,586 + +0.5 1,306,586 + -0.5 1,306,594 - -0.0 1,306,993 + SM_b20899 0.22 -2.4 1,307,253 - SM_b20899 0.48 -1.0 1,307,397 - SM_b20899 0.63 -0.6 1,307,471 + SM_b20899 0.70 -1.1 1,307,471 + SM_b20899 0.70 -0.8 1,307,471 + SM_b20899 0.70 +1.0 1,307,471 + SM_b20899 0.70 -1.7 1,307,472 - SM_b20899 0.70 -2.5 1,307,570 + SM_b20899 0.80 +2.3 1,307,570 + SM_b20899 0.80 -2.8 1,307,570 + SM_b20899 0.80 +0.9 1,307,571 - SM_b20899 0.80 -1.7 1,307,571 - SM_b20899 0.80 +0.3 1,307,609 + SM_b20899 0.84 -0.4 1,307,611 + SM_b20899 0.84 -1.1 1,307,622 - SM_b20899 0.85 -0.7 1,307,647 - SM_b20899 0.88 -1.0 1,307,832 + -0.9 1,307,832 + +1.0 1,307,832 + +1.4 1,307,832 + +0.2 1,307,832 + +0.8 1,307,833 - -0.1 1,307,833 - -0.3 1,308,033 - SM_b20900 0.10 -0.5 1,308,033 - SM_b20900 0.10 +0.3 1,308,280 - SM_b20900 0.23 -0.6 1,308,321 + SM_b20900 0.25 -0.0 1,308,453 + SM_b20900 0.32 +0.5 1,308,560 - SM_b20900 0.38 +1.1
Or see this region's nucleotide sequence