Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20827 and SM_b20828 are separated by 222 nucleotides SM_b20828 and SM_b21663 overlap by 4 nucleotides SM_b21663 and SM_b21664 are separated by 99 nucleotides SM_b21664 and SM_b21665 are separated by 57 nucleotides
SM_b20827: SM_b20827 - transposase, at 617,618 to 618,205
_b20827
SM_b20828: SM_b20828 - hypothetical protein, at 618,428 to 618,703
_b20828
SM_b21663: SM_b21663 - hypothetical protein, at 618,700 to 619,116
_b21663
SM_b21664: SM_b21664 - hypothetical protein, at 619,216 to 619,485
_b21664
SM_b21665: SM_b21665 - hypothetical protein, at 619,543 to 619,899
_b21665
Position (kb)
618
619 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 617.454 kb on + strand at 617.454 kb on + strand at 617.454 kb on + strand at 617.454 kb on + strand at 617.454 kb on + strand at 617.454 kb on + strand at 617.457 kb on + strand at 617.549 kb on + strand at 617.549 kb on + strand at 617.660 kb on + strand at 617.660 kb on + strand at 617.661 kb on - strand at 617.669 kb on + strand at 617.670 kb on - strand at 617.670 kb on - strand at 617.949 kb on - strand, within SM_b20827 at 617.949 kb on - strand at 617.963 kb on + strand, within SM_b20827 at 617.964 kb on - strand, within SM_b20827 at 618.128 kb on + strand, within SM_b20827 at 618.129 kb on - strand, within SM_b20827 at 618.196 kb on - strand at 618.198 kb on + strand at 618.198 kb on + strand at 618.198 kb on + strand at 618.199 kb on - strand at 618.204 kb on - strand at 618.204 kb on - strand at 618.354 kb on + strand at 618.355 kb on - strand at 618.394 kb on - strand at 618.479 kb on + strand, within SM_b20828 at 618.479 kb on + strand, within SM_b20828 at 618.479 kb on + strand, within SM_b20828 at 618.491 kb on + strand, within SM_b20828 at 618.492 kb on - strand, within SM_b20828 at 618.492 kb on - strand, within SM_b20828 at 618.492 kb on - strand, within SM_b20828 at 618.492 kb on - strand, within SM_b20828 at 618.537 kb on - strand, within SM_b20828 at 618.789 kb on + strand, within SM_b21663 at 618.941 kb on + strand, within SM_b21663 at 618.954 kb on - strand, within SM_b21663 at 619.084 kb on + strand at 619.085 kb on - strand at 619.231 kb on + strand at 619.293 kb on - strand, within SM_b21664 at 619.402 kb on + strand, within SM_b21664 at 619.402 kb on + strand, within SM_b21664 at 619.402 kb on + strand, within SM_b21664 at 619.402 kb on + strand, within SM_b21664 at 619.403 kb on - strand, within SM_b21664 at 619.403 kb on - strand, within SM_b21664 at 619.435 kb on + strand, within SM_b21664 at 619.435 kb on + strand, within SM_b21664 at 619.435 kb on + strand, within SM_b21664 at 619.436 kb on - strand, within SM_b21664 at 619.436 kb on - strand, within SM_b21664 at 619.436 kb on - strand, within SM_b21664 at 619.436 kb on - strand, within SM_b21664
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 617,454 + -0.4 617,454 + +0.9 617,454 + -0.9 617,454 + -0.8 617,454 + +0.0 617,454 + -0.1 617,457 + -0.2 617,549 + -2.1 617,549 + +0.4 617,660 + -0.8 617,660 + +0.6 617,661 - +0.5 617,669 + -0.1 617,670 - -3.6 617,670 - -0.4 617,949 - SM_b20827 0.56 -2.3 617,949 - -0.7 617,963 + SM_b20827 0.59 -0.7 617,964 - SM_b20827 0.59 +4.3 618,128 + SM_b20827 0.87 +0.9 618,129 - SM_b20827 0.87 +0.7 618,196 - -1.3 618,198 + -3.7 618,198 + -1.7 618,198 + +3.7 618,199 - -1.9 618,204 - -0.5 618,204 - +1.1 618,354 + -0.7 618,355 - -1.5 618,394 - -0.9 618,479 + SM_b20828 0.18 +1.1 618,479 + SM_b20828 0.18 +1.5 618,479 + SM_b20828 0.18 -0.2 618,491 + SM_b20828 0.23 -0.2 618,492 - SM_b20828 0.23 +2.1 618,492 - SM_b20828 0.23 +0.6 618,492 - SM_b20828 0.23 -1.5 618,492 - SM_b20828 0.23 +2.9 618,537 - SM_b20828 0.39 +0.2 618,789 + SM_b21663 0.21 -1.3 618,941 + SM_b21663 0.58 +0.4 618,954 - SM_b21663 0.61 -1.2 619,084 + +0.9 619,085 - -3.5 619,231 + +0.0 619,293 - SM_b21664 0.29 +0.9 619,402 + SM_b21664 0.69 +1.0 619,402 + SM_b21664 0.69 -2.9 619,402 + SM_b21664 0.69 +2.3 619,402 + SM_b21664 0.69 +1.3 619,403 - SM_b21664 0.69 +0.9 619,403 - SM_b21664 0.69 -0.7 619,435 + SM_b21664 0.81 -2.5 619,435 + SM_b21664 0.81 -0.9 619,435 + SM_b21664 0.81 -0.7 619,436 - SM_b21664 0.81 +0.5 619,436 - SM_b21664 0.81 +0.3 619,436 - SM_b21664 0.81 -0.1 619,436 - SM_b21664 0.81 -2.3
Or see this region's nucleotide sequence