Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20825 and SM_b20826 are separated by 67 nucleotides SM_b20826 and SM_b20827 are separated by 223 nucleotides
SM_b20825: SM_b20825 - acetyltransferase, cysElacA/lpxA/nodL family protein, at 616,023 to 616,793
_b20825
SM_b20826: SM_b20826 - hypothetical protein, at 616,861 to 617,394
_b20826
SM_b20827: SM_b20827 - transposase, at 617,618 to 618,205
_b20827
Position (kb)
616
617
618 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 615.872 kb on + strand at 615.872 kb on + strand at 615.873 kb on - strand at 615.893 kb on + strand at 615.894 kb on - strand at 615.956 kb on + strand at 615.971 kb on - strand at 616.036 kb on + strand at 616.037 kb on - strand at 616.037 kb on - strand at 616.037 kb on - strand at 616.037 kb on - strand at 616.037 kb on - strand at 616.058 kb on + strand at 616.058 kb on + strand at 616.059 kb on - strand at 616.059 kb on - strand at 616.059 kb on - strand at 616.059 kb on - strand at 616.070 kb on + strand at 616.070 kb on + strand at 616.071 kb on - strand at 616.071 kb on - strand at 616.071 kb on - strand at 616.088 kb on + strand at 616.089 kb on - strand at 616.139 kb on + strand, within SM_b20825 at 616.140 kb on - strand at 616.191 kb on + strand, within SM_b20825 at 616.192 kb on - strand, within SM_b20825 at 616.290 kb on - strand, within SM_b20825 at 616.296 kb on - strand, within SM_b20825 at 616.296 kb on - strand, within SM_b20825 at 616.478 kb on + strand, within SM_b20825 at 616.479 kb on - strand, within SM_b20825 at 616.479 kb on - strand, within SM_b20825 at 616.535 kb on + strand, within SM_b20825 at 616.536 kb on - strand, within SM_b20825 at 616.554 kb on + strand, within SM_b20825 at 616.554 kb on + strand, within SM_b20825 at 616.555 kb on - strand, within SM_b20825 at 616.555 kb on - strand, within SM_b20825 at 616.697 kb on + strand, within SM_b20825 at 616.698 kb on - strand, within SM_b20825 at 616.740 kb on + strand at 616.763 kb on + strand at 616.858 kb on + strand at 616.859 kb on - strand at 616.859 kb on - strand at 616.990 kb on + strand, within SM_b20826 at 616.990 kb on + strand, within SM_b20826 at 616.990 kb on + strand, within SM_b20826 at 616.990 kb on + strand, within SM_b20826 at 617.035 kb on + strand, within SM_b20826 at 617.035 kb on + strand, within SM_b20826 at 617.054 kb on + strand, within SM_b20826 at 617.054 kb on + strand, within SM_b20826 at 617.115 kb on + strand, within SM_b20826 at 617.116 kb on - strand, within SM_b20826 at 617.302 kb on + strand, within SM_b20826 at 617.302 kb on + strand, within SM_b20826 at 617.303 kb on - strand, within SM_b20826 at 617.323 kb on + strand, within SM_b20826 at 617.323 kb on + strand, within SM_b20826 at 617.324 kb on - strand, within SM_b20826 at 617.328 kb on - strand, within SM_b20826 at 617.328 kb on - strand, within SM_b20826 at 617.403 kb on + strand at 617.403 kb on + strand at 617.454 kb on + strand at 617.454 kb on + strand at 617.454 kb on + strand at 617.454 kb on + strand at 617.454 kb on + strand at 617.454 kb on + strand at 617.457 kb on + strand at 617.549 kb on + strand at 617.549 kb on + strand at 617.660 kb on + strand at 617.660 kb on + strand at 617.661 kb on - strand at 617.669 kb on + strand at 617.670 kb on - strand at 617.670 kb on - strand at 617.949 kb on - strand, within SM_b20827 at 617.949 kb on - strand at 617.963 kb on + strand, within SM_b20827 at 617.964 kb on - strand, within SM_b20827 at 618.128 kb on + strand, within SM_b20827 at 618.129 kb on - strand, within SM_b20827 at 618.196 kb on - strand at 618.198 kb on + strand at 618.198 kb on + strand at 618.198 kb on + strand at 618.199 kb on - strand at 618.204 kb on - strand at 618.204 kb on - strand at 618.354 kb on + strand at 618.355 kb on - strand at 618.394 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 615,872 + +0.5 615,872 + +2.1 615,873 - -0.5 615,893 + +0.6 615,894 - +2.2 615,956 + +0.9 615,971 - +1.1 616,036 + -2.5 616,037 - +1.2 616,037 - -1.5 616,037 - -0.7 616,037 - -0.9 616,037 - -3.5 616,058 + -0.9 616,058 + +2.3 616,059 - -0.1 616,059 - +2.1 616,059 - +2.1 616,059 - -0.3 616,070 + +0.3 616,070 + +0.3 616,071 - +4.3 616,071 - +0.7 616,071 - +0.1 616,088 + -0.3 616,089 - -0.7 616,139 + SM_b20825 0.15 -1.3 616,140 - -0.7 616,191 + SM_b20825 0.22 +1.7 616,192 - SM_b20825 0.22 +0.4 616,290 - SM_b20825 0.35 +1.3 616,296 - SM_b20825 0.35 -0.2 616,296 - SM_b20825 0.35 -1.0 616,478 + SM_b20825 0.59 -1.0 616,479 - SM_b20825 0.59 -2.0 616,479 - SM_b20825 0.59 -1.7 616,535 + SM_b20825 0.66 -1.5 616,536 - SM_b20825 0.67 -0.5 616,554 + SM_b20825 0.69 -2.8 616,554 + SM_b20825 0.69 -2.8 616,555 - SM_b20825 0.69 +1.8 616,555 - SM_b20825 0.69 -2.4 616,697 + SM_b20825 0.87 +2.3 616,698 - SM_b20825 0.88 -1.1 616,740 + -0.7 616,763 + -0.6 616,858 + +1.1 616,859 - -1.5 616,859 - -2.1 616,990 + SM_b20826 0.24 +1.5 616,990 + SM_b20826 0.24 -1.3 616,990 + SM_b20826 0.24 -2.1 616,990 + SM_b20826 0.24 +0.7 617,035 + SM_b20826 0.33 -1.5 617,035 + SM_b20826 0.33 -0.5 617,054 + SM_b20826 0.36 -0.4 617,054 + SM_b20826 0.36 -2.6 617,115 + SM_b20826 0.48 -0.0 617,116 - SM_b20826 0.48 +0.2 617,302 + SM_b20826 0.83 +0.2 617,302 + SM_b20826 0.83 -0.8 617,303 - SM_b20826 0.83 +0.6 617,323 + SM_b20826 0.87 -2.5 617,323 + SM_b20826 0.87 +0.7 617,324 - SM_b20826 0.87 -2.6 617,328 - SM_b20826 0.87 -1.0 617,328 - SM_b20826 0.87 -4.3 617,403 + -0.8 617,403 + +1.3 617,454 + -0.4 617,454 + +0.9 617,454 + -0.9 617,454 + -0.8 617,454 + +0.0 617,454 + -0.1 617,457 + -0.2 617,549 + -2.1 617,549 + +0.4 617,660 + -0.8 617,660 + +0.6 617,661 - +0.5 617,669 + -0.1 617,670 - -3.6 617,670 - -0.4 617,949 - SM_b20827 0.56 -2.3 617,949 - -0.7 617,963 + SM_b20827 0.59 -0.7 617,964 - SM_b20827 0.59 +4.3 618,128 + SM_b20827 0.87 +0.9 618,129 - SM_b20827 0.87 +0.7 618,196 - -1.3 618,198 + -3.7 618,198 + -1.7 618,198 + +3.7 618,199 - -1.9 618,204 - -0.5 618,204 - +1.1 618,354 + -0.7 618,355 - -1.5 618,394 - -0.9
Or see this region's nucleotide sequence