Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20749 and SM_b20750 are separated by 19 nucleotides SM_b20750 and SM_b20751 are separated by 30 nucleotides SM_b20751 and SM_b20752 are separated by 46 nucleotides
SM_b20749: SM_b20749 - D-mannonate oxidoreductase, at 1,538,076 to 1,539,539
_b20749
SM_b20750: SM_b20750 - dehydrogenase, at 1,539,559 to 1,540,308
_b20750
SM_b20751: SM_b20751 - 3-hydroxyisobutyrate dehydrogenase, at 1,540,339 to 1,541,235
_b20751
SM_b20752: SM_b20752 - 3-hydroxyisobutyryl-CoA hydrolase, at 1,541,282 to 1,542,352
_b20752
Position (kb) 1540
1541
1542 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1539.537 kb on + strand at 1540.015 kb on + strand, within SM_b20750 at 1540.018 kb on - strand, within SM_b20750 at 1540.235 kb on + strand at 1540.430 kb on + strand, within SM_b20751 at 1540.430 kb on + strand, within SM_b20751 at 1540.589 kb on + strand, within SM_b20751 at 1540.589 kb on + strand, within SM_b20751 at 1540.589 kb on + strand, within SM_b20751 at 1540.590 kb on - strand, within SM_b20751 at 1540.623 kb on + strand, within SM_b20751 at 1540.623 kb on + strand, within SM_b20751 at 1540.852 kb on - strand, within SM_b20751 at 1541.018 kb on + strand, within SM_b20751 at 1541.284 kb on - strand at 1541.284 kb on - strand at 1541.347 kb on + strand at 1541.856 kb on + strand, within SM_b20752 at 1541.856 kb on + strand, within SM_b20752 at 1541.856 kb on + strand, within SM_b20752 at 1541.857 kb on - strand, within SM_b20752 at 1541.860 kb on + strand, within SM_b20752 at 1542.032 kb on - strand, within SM_b20752 at 1542.033 kb on + strand, within SM_b20752 at 1542.033 kb on + strand, within SM_b20752 at 1542.034 kb on - strand, within SM_b20752 at 1542.046 kb on - strand, within SM_b20752 at 1542.127 kb on - strand, within SM_b20752 at 1542.127 kb on - strand, within SM_b20752 at 1542.128 kb on + strand, within SM_b20752 at 1542.129 kb on - strand, within SM_b20752
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,539,537 + +0.4 1,540,015 + SM_b20750 0.61 -0.4 1,540,018 - SM_b20750 0.61 +0.4 1,540,235 + -2.4 1,540,430 + SM_b20751 0.10 +0.3 1,540,430 + SM_b20751 0.10 +1.6 1,540,589 + SM_b20751 0.28 -0.1 1,540,589 + SM_b20751 0.28 +1.3 1,540,589 + SM_b20751 0.28 +2.3 1,540,590 - SM_b20751 0.28 -1.1 1,540,623 + SM_b20751 0.32 +0.2 1,540,623 + SM_b20751 0.32 +2.1 1,540,852 - SM_b20751 0.57 +0.0 1,541,018 + SM_b20751 0.76 +0.2 1,541,284 - -0.5 1,541,284 - -1.3 1,541,347 + -0.3 1,541,856 + SM_b20752 0.54 -0.9 1,541,856 + SM_b20752 0.54 +3.0 1,541,856 + SM_b20752 0.54 +0.3 1,541,857 - SM_b20752 0.54 +0.3 1,541,860 + SM_b20752 0.54 +0.3 1,542,032 - SM_b20752 0.70 +1.2 1,542,033 + SM_b20752 0.70 +1.3 1,542,033 + SM_b20752 0.70 -2.4 1,542,034 - SM_b20752 0.70 +1.0 1,542,046 - SM_b20752 0.71 +1.3 1,542,127 - SM_b20752 0.79 -1.6 1,542,127 - SM_b20752 0.79 -1.2 1,542,128 + SM_b20752 0.79 +0.3 1,542,129 - SM_b20752 0.79 +2.4
Or see this region's nucleotide sequence