Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20617 and SM_b20618 overlap by 4 nucleotides SM_b20618 and SM_b20619 are separated by 69 nucleotides SM_b20619 and SM_b20620 are separated by 42 nucleotides
SM_b20617: SM_b20617 - thiazole synthase, at 1,636,955 to 1,637,728
_b20617
SM_b20618: SM_b20618 - thiamine-phosphate pyrophosphorylase, at 1,637,725 to 1,638,330
_b20618
SM_b20619: SM_b20619 - regulatory protein, at 1,638,400 to 1,639,389
_b20619
SM_b20620: SM_b20620 - sugar uptake ABC transporter substrate-binding protein precursor, at 1,639,432 to 1,640,475
_b20620
Position (kb)
1638
1639
1640 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1637.743 kb on + strand at 1637.852 kb on + strand, within SM_b20618 at 1637.853 kb on - strand, within SM_b20618 at 1637.924 kb on + strand, within SM_b20618 at 1638.092 kb on - strand, within SM_b20618 at 1638.202 kb on - strand, within SM_b20618 at 1638.328 kb on + strand at 1638.485 kb on - strand at 1638.485 kb on - strand at 1638.487 kb on + strand at 1638.488 kb on - strand at 1638.488 kb on - strand at 1638.488 kb on - strand at 1638.489 kb on + strand at 1638.489 kb on + strand at 1638.490 kb on - strand at 1638.490 kb on - strand at 1638.490 kb on - strand at 1638.557 kb on - strand, within SM_b20619 at 1638.557 kb on - strand, within SM_b20619 at 1638.557 kb on - strand, within SM_b20619 at 1638.557 kb on - strand, within SM_b20619 at 1638.557 kb on - strand, within SM_b20619 at 1638.557 kb on - strand, within SM_b20619 at 1638.584 kb on + strand, within SM_b20619 at 1638.699 kb on - strand, within SM_b20619 at 1638.794 kb on + strand, within SM_b20619 at 1638.794 kb on + strand, within SM_b20619 at 1638.794 kb on + strand, within SM_b20619 at 1638.795 kb on - strand, within SM_b20619 at 1638.795 kb on - strand, within SM_b20619 at 1638.795 kb on - strand, within SM_b20619 at 1638.797 kb on - strand, within SM_b20619 at 1638.804 kb on - strand, within SM_b20619 at 1638.877 kb on + strand, within SM_b20619 at 1638.878 kb on - strand, within SM_b20619 at 1638.883 kb on + strand, within SM_b20619 at 1639.051 kb on + strand, within SM_b20619 at 1639.051 kb on + strand, within SM_b20619 at 1639.052 kb on - strand, within SM_b20619 at 1639.392 kb on - strand at 1639.429 kb on + strand at 1639.430 kb on - strand at 1639.722 kb on - strand, within SM_b20620 at 1639.913 kb on + strand, within SM_b20620 at 1639.977 kb on - strand, within SM_b20620 at 1639.982 kb on + strand, within SM_b20620 at 1639.983 kb on - strand, within SM_b20620 at 1639.983 kb on - strand, within SM_b20620 at 1640.176 kb on - strand, within SM_b20620 at 1640.201 kb on - strand, within SM_b20620 at 1640.218 kb on - strand, within SM_b20620 at 1640.218 kb on - strand, within SM_b20620 at 1640.306 kb on + strand, within SM_b20620 at 1640.307 kb on - strand, within SM_b20620 at 1640.336 kb on + strand, within SM_b20620
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,637,743 + +0.7 1,637,852 + SM_b20618 0.21 +0.9 1,637,853 - SM_b20618 0.21 +1.4 1,637,924 + SM_b20618 0.33 +1.8 1,638,092 - SM_b20618 0.61 -1.9 1,638,202 - SM_b20618 0.79 +0.8 1,638,328 + +0.0 1,638,485 - -1.1 1,638,485 - +1.0 1,638,487 + -1.8 1,638,488 - +1.2 1,638,488 - +1.1 1,638,488 - +1.1 1,638,489 + +0.1 1,638,489 + +2.6 1,638,490 - -0.3 1,638,490 - +0.5 1,638,490 - -0.9 1,638,557 - SM_b20619 0.16 -2.0 1,638,557 - SM_b20619 0.16 +0.6 1,638,557 - SM_b20619 0.16 +0.9 1,638,557 - SM_b20619 0.16 +1.4 1,638,557 - SM_b20619 0.16 +1.9 1,638,557 - SM_b20619 0.16 -1.4 1,638,584 + SM_b20619 0.19 +0.8 1,638,699 - SM_b20619 0.30 +3.8 1,638,794 + SM_b20619 0.40 -0.8 1,638,794 + SM_b20619 0.40 -0.2 1,638,794 + SM_b20619 0.40 +0.9 1,638,795 - SM_b20619 0.40 -0.8 1,638,795 - SM_b20619 0.40 +2.2 1,638,795 - SM_b20619 0.40 +0.8 1,638,797 - SM_b20619 0.40 +4.8 1,638,804 - SM_b20619 0.41 +0.7 1,638,877 + SM_b20619 0.48 +0.4 1,638,878 - SM_b20619 0.48 -0.8 1,638,883 + SM_b20619 0.49 +0.8 1,639,051 + SM_b20619 0.66 -2.2 1,639,051 + SM_b20619 0.66 +0.9 1,639,052 - SM_b20619 0.66 +0.2 1,639,392 - +0.9 1,639,429 + -0.1 1,639,430 - -2.8 1,639,722 - SM_b20620 0.28 +0.3 1,639,913 + SM_b20620 0.46 -1.5 1,639,977 - SM_b20620 0.52 +0.6 1,639,982 + SM_b20620 0.53 -1.3 1,639,983 - SM_b20620 0.53 +4.2 1,639,983 - SM_b20620 0.53 -0.7 1,640,176 - SM_b20620 0.71 -1.8 1,640,201 - SM_b20620 0.74 -0.5 1,640,218 - SM_b20620 0.75 +2.2 1,640,218 - SM_b20620 0.75 -1.1 1,640,306 + SM_b20620 0.84 -1.1 1,640,307 - SM_b20620 0.84 +0.1 1,640,336 + SM_b20620 0.87 +1.6
Or see this region's nucleotide sequence