Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20575 and SM_b20576 are separated by 82 nucleotides SM_b20576 and SM_b20577 are separated by 10 nucleotides SM_b20577 and SM_b20578 are separated by 16 nucleotides
SM_b20575: SM_b20575 - 3-carboxy-cis,cis-muconate cycloisomerase, at 1,589,460 to 1,590,515
_b20575
SM_b20576: SM_b20576 - protocatechuate 3,4-dioxygenase subunit alpha, at 1,590,598 to 1,591,212
_b20576
SM_b20577: SM_b20577 - protocatechuate 3,4-dioxygenase subunit beta, at 1,591,223 to 1,591,984
_b20577
SM_b20578: SM_b20578 - gamma carboxymuconolactone decarboxylase, at 1,592,001 to 1,592,408
_b20578
Position (kb)
1590
1591
1592 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1590.177 kb on - strand, within SM_b20575 at 1590.487 kb on + strand at 1590.653 kb on + strand at 1590.654 kb on - strand at 1590.654 kb on - strand at 1590.654 kb on - strand at 1590.657 kb on + strand at 1590.658 kb on - strand at 1590.758 kb on + strand, within SM_b20576 at 1590.758 kb on + strand, within SM_b20576 at 1590.759 kb on - strand, within SM_b20576 at 1590.759 kb on - strand, within SM_b20576 at 1590.759 kb on - strand, within SM_b20576 at 1590.759 kb on - strand, within SM_b20576 at 1590.780 kb on + strand, within SM_b20576 at 1590.965 kb on + strand, within SM_b20576 at 1590.965 kb on + strand, within SM_b20576 at 1591.109 kb on + strand, within SM_b20576 at 1591.112 kb on - strand, within SM_b20576 at 1591.157 kb on + strand at 1591.157 kb on + strand at 1591.158 kb on - strand at 1591.158 kb on - strand at 1591.212 kb on + strand at 1591.416 kb on + strand, within SM_b20577 at 1591.416 kb on + strand, within SM_b20577 at 1591.416 kb on + strand, within SM_b20577 at 1591.416 kb on + strand, within SM_b20577 at 1591.472 kb on - strand, within SM_b20577 at 1591.513 kb on - strand, within SM_b20577 at 1591.759 kb on + strand, within SM_b20577 at 1591.759 kb on + strand, within SM_b20577 at 1592.086 kb on + strand, within SM_b20578 at 1592.086 kb on + strand, within SM_b20578 at 1592.087 kb on - strand, within SM_b20578 at 1592.087 kb on - strand, within SM_b20578 at 1592.087 kb on - strand, within SM_b20578 at 1592.117 kb on + strand, within SM_b20578 at 1592.118 kb on - strand, within SM_b20578
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 1,590,177 - SM_b20575 0.68 +0.7 1,590,487 + -1.8 1,590,653 + +0.1 1,590,654 - +0.5 1,590,654 - +2.1 1,590,654 - +0.2 1,590,657 + +0.4 1,590,658 - -1.4 1,590,758 + SM_b20576 0.26 +0.3 1,590,758 + SM_b20576 0.26 +4.4 1,590,759 - SM_b20576 0.26 +0.4 1,590,759 - SM_b20576 0.26 -1.2 1,590,759 - SM_b20576 0.26 +0.2 1,590,759 - SM_b20576 0.26 +0.2 1,590,780 + SM_b20576 0.30 +1.4 1,590,965 + SM_b20576 0.60 -0.1 1,590,965 + SM_b20576 0.60 +1.5 1,591,109 + SM_b20576 0.83 +0.5 1,591,112 - SM_b20576 0.84 +0.7 1,591,157 + -0.2 1,591,157 + +0.8 1,591,158 - -2.1 1,591,158 - -0.8 1,591,212 + +0.3 1,591,416 + SM_b20577 0.25 -0.3 1,591,416 + SM_b20577 0.25 -2.3 1,591,416 + SM_b20577 0.25 -1.8 1,591,416 + SM_b20577 0.25 -1.1 1,591,472 - SM_b20577 0.33 -1.9 1,591,513 - SM_b20577 0.38 +0.6 1,591,759 + SM_b20577 0.70 -0.5 1,591,759 + SM_b20577 0.70 +0.6 1,592,086 + SM_b20578 0.21 -0.2 1,592,086 + SM_b20578 0.21 +1.5 1,592,087 - SM_b20578 0.21 -1.9 1,592,087 - SM_b20578 0.21 -0.6 1,592,087 - SM_b20578 0.21 +0.9 1,592,117 + SM_b20578 0.28 +1.0 1,592,118 - SM_b20578 0.29 -2.8
Or see this region's nucleotide sequence