Experiment: Polymyxin B sulfate 0.00005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20522 and SM_b20523 are separated by 473 nucleotides SM_b20523 and SM_b20525 are separated by 517 nucleotides
SM_b20522: SM_b20522 - hypothetical protein, at 548,378 to 548,746
_b20522
SM_b20523: SM_b20523 - hypothetical protein, at 549,220 to 550,428
_b20523
SM_b20525: SM_b20525 - transcriptional regulator, at 550,946 to 551,554
_b20525
Position (kb) 549
550
551 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 548.353 kb on - strand at 548.363 kb on + strand at 548.414 kb on - strand at 548.433 kb on + strand, within SM_b20522 at 548.434 kb on - strand, within SM_b20522 at 548.476 kb on + strand, within SM_b20522 at 548.519 kb on + strand, within SM_b20522 at 548.520 kb on - strand, within SM_b20522 at 548.520 kb on - strand, within SM_b20522 at 548.520 kb on - strand, within SM_b20522 at 548.544 kb on + strand, within SM_b20522 at 548.594 kb on + strand, within SM_b20522 at 548.595 kb on - strand, within SM_b20522 at 548.595 kb on - strand, within SM_b20522 at 548.595 kb on - strand, within SM_b20522 at 548.678 kb on + strand, within SM_b20522 at 548.679 kb on - strand, within SM_b20522 at 548.712 kb on - strand at 548.717 kb on + strand at 548.718 kb on - strand at 548.718 kb on - strand at 548.720 kb on + strand at 548.720 kb on + strand at 548.721 kb on - strand at 548.721 kb on - strand at 548.727 kb on + strand at 548.727 kb on + strand at 548.728 kb on - strand at 548.736 kb on - strand at 548.736 kb on - strand at 548.743 kb on - strand at 548.743 kb on - strand at 548.928 kb on + strand at 548.948 kb on + strand at 548.949 kb on - strand at 548.973 kb on - strand at 549.063 kb on + strand at 549.064 kb on - strand at 549.110 kb on - strand at 549.161 kb on - strand at 549.186 kb on + strand at 549.222 kb on - strand at 549.234 kb on - strand at 549.246 kb on + strand at 549.246 kb on + strand at 549.246 kb on + strand at 549.246 kb on + strand at 549.246 kb on + strand at 549.246 kb on + strand at 549.247 kb on - strand at 549.247 kb on - strand at 549.247 kb on - strand at 549.251 kb on + strand at 549.295 kb on + strand at 549.326 kb on + strand at 549.337 kb on - strand at 549.337 kb on - strand at 549.473 kb on - strand, within SM_b20523 at 549.476 kb on - strand, within SM_b20523 at 549.476 kb on - strand, within SM_b20523 at 549.562 kb on + strand, within SM_b20523 at 549.562 kb on + strand, within SM_b20523 at 549.562 kb on + strand, within SM_b20523 at 549.562 kb on + strand, within SM_b20523 at 549.562 kb on + strand, within SM_b20523 at 549.563 kb on - strand, within SM_b20523 at 549.563 kb on - strand, within SM_b20523 at 549.563 kb on - strand, within SM_b20523 at 549.577 kb on - strand, within SM_b20523 at 549.927 kb on - strand, within SM_b20523 at 549.944 kb on + strand, within SM_b20523 at 549.976 kb on + strand, within SM_b20523 at 549.977 kb on - strand, within SM_b20523 at 550.097 kb on + strand, within SM_b20523 at 550.097 kb on + strand, within SM_b20523 at 550.097 kb on + strand, within SM_b20523 at 550.097 kb on + strand, within SM_b20523 at 550.098 kb on - strand, within SM_b20523 at 550.098 kb on - strand, within SM_b20523 at 550.257 kb on + strand, within SM_b20523 at 550.298 kb on - strand, within SM_b20523 at 550.442 kb on + strand at 550.443 kb on - strand at 550.444 kb on + strand at 550.445 kb on - strand at 550.445 kb on - strand at 550.445 kb on - strand at 550.445 kb on - strand at 550.499 kb on + strand at 550.499 kb on + strand at 550.499 kb on + strand at 550.500 kb on - strand at 550.500 kb on - strand at 550.500 kb on - strand at 550.524 kb on + strand at 550.524 kb on + strand at 550.525 kb on - strand at 550.805 kb on - strand at 550.805 kb on - strand at 550.831 kb on + strand at 550.832 kb on - strand at 550.832 kb on - strand at 550.898 kb on + strand at 550.898 kb on + strand at 550.898 kb on + strand at 550.899 kb on - strand at 551.049 kb on + strand, within SM_b20525 at 551.073 kb on - strand, within SM_b20525 at 551.100 kb on + strand, within SM_b20525 at 551.101 kb on - strand, within SM_b20525 at 551.101 kb on - strand, within SM_b20525 at 551.206 kb on + strand, within SM_b20525 at 551.206 kb on + strand, within SM_b20525 at 551.206 kb on + strand, within SM_b20525 at 551.206 kb on + strand, within SM_b20525 at 551.394 kb on + strand, within SM_b20525 at 551.394 kb on + strand, within SM_b20525 at 551.394 kb on + strand, within SM_b20525 at 551.395 kb on - strand, within SM_b20525 at 551.395 kb on - strand, within SM_b20525 at 551.395 kb on - strand, within SM_b20525 at 551.404 kb on - strand, within SM_b20525
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.00005 mg/ml remove 548,353 - -0.6 548,363 + -1.0 548,414 - +2.0 548,433 + SM_b20522 0.15 -0.6 548,434 - SM_b20522 0.15 +0.9 548,476 + SM_b20522 0.27 +1.9 548,519 + SM_b20522 0.38 -2.9 548,520 - SM_b20522 0.38 -2.9 548,520 - SM_b20522 0.38 +0.5 548,520 - SM_b20522 0.38 +0.5 548,544 + SM_b20522 0.45 -0.5 548,594 + SM_b20522 0.59 -1.4 548,595 - SM_b20522 0.59 -0.3 548,595 - SM_b20522 0.59 -0.2 548,595 - SM_b20522 0.59 +1.2 548,678 + SM_b20522 0.81 +1.2 548,679 - SM_b20522 0.82 +1.1 548,712 - +0.2 548,717 + +0.9 548,718 - +1.5 548,718 - -3.3 548,720 + +1.8 548,720 + +0.3 548,721 - -3.1 548,721 - +1.0 548,727 + +0.0 548,727 + +0.1 548,728 - +0.1 548,736 - +0.5 548,736 - -0.5 548,743 - +0.9 548,743 - -0.1 548,928 + +0.3 548,948 + +1.8 548,949 - -1.6 548,973 - +0.5 549,063 + -0.3 549,064 - -0.3 549,110 - +0.7 549,161 - +1.6 549,186 + +2.5 549,222 - +0.4 549,234 - -0.9 549,246 + +0.9 549,246 + -0.9 549,246 + +2.7 549,246 + +1.4 549,246 + -2.8 549,246 + +1.9 549,247 - -3.9 549,247 - +0.5 549,247 - -2.3 549,251 + -0.4 549,295 + +0.6 549,326 + +1.0 549,337 - +0.3 549,337 - +0.1 549,473 - SM_b20523 0.21 -0.6 549,476 - SM_b20523 0.21 +1.5 549,476 - SM_b20523 0.21 +0.1 549,562 + SM_b20523 0.28 -1.7 549,562 + SM_b20523 0.28 +0.5 549,562 + SM_b20523 0.28 -1.5 549,562 + SM_b20523 0.28 +0.0 549,562 + SM_b20523 0.28 +1.3 549,563 - SM_b20523 0.28 +0.6 549,563 - SM_b20523 0.28 -2.1 549,563 - SM_b20523 0.28 +0.3 549,577 - SM_b20523 0.30 +0.4 549,927 - SM_b20523 0.58 -0.1 549,944 + SM_b20523 0.60 -0.2 549,976 + SM_b20523 0.63 +1.6 549,977 - SM_b20523 0.63 +1.1 550,097 + SM_b20523 0.73 +0.7 550,097 + SM_b20523 0.73 +0.7 550,097 + SM_b20523 0.73 +0.2 550,097 + SM_b20523 0.73 -1.9 550,098 - SM_b20523 0.73 +0.6 550,098 - SM_b20523 0.73 +0.9 550,257 + SM_b20523 0.86 -0.5 550,298 - SM_b20523 0.89 -0.3 550,442 + +0.9 550,443 - +1.1 550,444 + +0.5 550,445 - -3.4 550,445 - +0.6 550,445 - -0.7 550,445 - -1.1 550,499 + +0.9 550,499 + +0.7 550,499 + +1.4 550,500 - -0.9 550,500 - -1.7 550,500 - -1.8 550,524 + +0.6 550,524 + -0.6 550,525 - -1.2 550,805 - -2.3 550,805 - -0.7 550,831 + +0.7 550,832 - +0.6 550,832 - +0.8 550,898 + -2.3 550,898 + +0.1 550,898 + -0.6 550,899 - +0.8 551,049 + SM_b20525 0.17 -0.7 551,073 - SM_b20525 0.21 +0.9 551,100 + SM_b20525 0.25 +1.2 551,101 - SM_b20525 0.25 +0.9 551,101 - SM_b20525 0.25 -1.3 551,206 + SM_b20525 0.43 +1.1 551,206 + SM_b20525 0.43 +1.2 551,206 + SM_b20525 0.43 +4.1 551,206 + SM_b20525 0.43 -2.1 551,394 + SM_b20525 0.74 -0.2 551,394 + SM_b20525 0.74 -0.1 551,394 + SM_b20525 0.74 -1.6 551,395 - SM_b20525 0.74 +0.4 551,395 - SM_b20525 0.74 -2.0 551,395 - SM_b20525 0.74 -0.0 551,404 - SM_b20525 0.75 -2.9
Or see this region's nucleotide sequence